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Q10002

- APN1_CAEEL

UniProt

Q10002 - APN1_CAEEL

Protein

DNA-(apurinic or apyrimidinic site) lyase

Gene

apn-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (19 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

    Cofactori

    Binds 3 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi185 – 1851Zinc 1By similarity
    Metal bindingi225 – 2251Zinc 1By similarity
    Metal bindingi261 – 2611Zinc 1By similarity
    Metal bindingi261 – 2611Zinc 2By similarity
    Metal bindingi295 – 2951Zinc 2By similarity
    Metal bindingi298 – 2981Zinc 3By similarity
    Metal bindingi332 – 3321Zinc 2By similarity
    Metal bindingi345 – 3451Zinc 3By similarity
    Metal bindingi347 – 3471Zinc 3By similarity
    Metal bindingi377 – 3771Zinc 2By similarity

    GO - Molecular functioni

    1. DNA-(apurinic or apyrimidinic site) lyase activity Source: WormBase
    2. DNA binding Source: InterPro
    3. phosphoric diester hydrolase activity Source: WormBase
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. base-excision repair Source: WormBase
    2. DNA catabolic process, endonucleolytic Source: GOC

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
    Alternative name(s):
    Apurinic-apyrimidinic endonuclease
    Short name:
    AP endonuclease
    Gene namesi
    Name:apn-1
    ORF Names:T05H10.2
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome II

    Organism-specific databases

    WormBaseiT05H10.2; CE28953; WBGene00000151; apn-1.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mitochondrion Source: RefGenome
    2. nucleus Source: RefGenome

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 396396DNA-(apurinic or apyrimidinic site) lyasePRO_0000190899Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi6239.T05H10.2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ10002.
    SMRiQ10002. Positions 118-378.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AP endonuclease 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG0648.
    GeneTreeiENSGT00390000013698.
    HOGENOMiHOG000224893.
    InParanoidiQ10002.
    KOiK10771.
    OMAiNTERKAD.
    PhylomeDBiQ10002.

    Family and domain databases

    Gene3Di3.20.20.150. 1 hit.
    HAMAPiMF_00152. Nfo.
    InterProiIPR001719. AP_endonuc_2.
    IPR018246. AP_endonuc_F2_Zn_BS.
    IPR013022. Xyl_isomerase-like_TIM-brl.
    [Graphical view]
    PANTHERiPTHR21445. PTHR21445. 1 hit.
    PfamiPF01261. AP_endonuc_2. 1 hit.
    [Graphical view]
    SMARTiSM00518. AP2Ec. 1 hit.
    [Graphical view]
    SUPFAMiSSF51658. SSF51658. 1 hit.
    TIGRFAMsiTIGR00587. nfo. 1 hit.
    PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
    PS00730. AP_NUCLEASE_F2_2. 1 hit.
    PS00731. AP_NUCLEASE_F2_3. 1 hit.
    PS51432. AP_NUCLEASE_F2_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q10002-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANKKVTFRE DVKSPAIRKL KQKLTPLKIK KGRGKIQKHI QKTLQKMKEE    50
    EESENQSPGT TVEETLTEEN ISTDKEETSK LENKPKKTRK TSGETIAQKK 100
    SRETVGVEVL KTSEGSSKML GFHVSAAGGL EQAIYNARAE GCRSFAMFVR 150
    NQRTWNHKPM SEEVVENWWK AVRETNFPLD QIVPHGSYLM NAGSPEAEKL 200
    EKSRLAMLDE CQRAEKLGIT MYNFHPGSTV GKCEKEECMT TIAETIDFVV 250
    EKTENIILVL ETMAGQGNSI GGTFEELKFI IDKVKVKSRV GVCIDTCHIF 300
    AGGYDIRTQK AYEEVMKNFG EVVGWNYLKA IHINDSKGDV GSKLDRHEHI 350
    GQGKIGKAAF ELLMNDNRLD GIPMILETPE GKYPEEMMIM YNMDKR 396
    Length:396
    Mass (Da):44,747
    Last modified:December 19, 2001 - v2
    Checksum:i0C1D1B26AB35BA15
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z47812 Genomic DNA. Translation: CAA87789.2.
    U40707 mRNA. Translation: AAB39924.1.
    PIRiJC5235.
    RefSeqiNP_495687.1. NM_063286.5.
    UniGeneiCel.32771.

    Genome annotation databases

    EnsemblMetazoaiT05H10.2; T05H10.2; WBGene00000151.
    GeneIDi174291.
    KEGGicel:CELE_T05H10.2.
    UCSCiT05H10.2. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z47812 Genomic DNA. Translation: CAA87789.2 .
    U40707 mRNA. Translation: AAB39924.1 .
    PIRi JC5235.
    RefSeqi NP_495687.1. NM_063286.5.
    UniGenei Cel.32771.

    3D structure databases

    ProteinModelPortali Q10002.
    SMRi Q10002. Positions 118-378.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6239.T05H10.2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai T05H10.2 ; T05H10.2 ; WBGene00000151 .
    GeneIDi 174291.
    KEGGi cel:CELE_T05H10.2.
    UCSCi T05H10.2. c. elegans.

    Organism-specific databases

    CTDi 174291.
    WormBasei T05H10.2 ; CE28953 ; WBGene00000151 ; apn-1.

    Phylogenomic databases

    eggNOGi COG0648.
    GeneTreei ENSGT00390000013698.
    HOGENOMi HOG000224893.
    InParanoidi Q10002.
    KOi K10771.
    OMAi NTERKAD.
    PhylomeDBi Q10002.

    Miscellaneous databases

    NextBioi 883392.
    PROi Q10002.

    Family and domain databases

    Gene3Di 3.20.20.150. 1 hit.
    HAMAPi MF_00152. Nfo.
    InterProi IPR001719. AP_endonuc_2.
    IPR018246. AP_endonuc_F2_Zn_BS.
    IPR013022. Xyl_isomerase-like_TIM-brl.
    [Graphical view ]
    PANTHERi PTHR21445. PTHR21445. 1 hit.
    Pfami PF01261. AP_endonuc_2. 1 hit.
    [Graphical view ]
    SMARTi SM00518. AP2Ec. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51658. SSF51658. 1 hit.
    TIGRFAMsi TIGR00587. nfo. 1 hit.
    PROSITEi PS00729. AP_NUCLEASE_F2_1. 1 hit.
    PS00730. AP_NUCLEASE_F2_2. 1 hit.
    PS00731. AP_NUCLEASE_F2_3. 1 hit.
    PS51432. AP_NUCLEASE_F2_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    2. "The Caenorhabditis elegans gene CeAPN1 encodes a homolog of Escherichia coli and yeast apurinic/apyrimidinic endonuclease."
      Masson J.Y., Tremblay S., Ramotar D.
      Gene 179:291-293(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 104-396.

    Entry informationi

    Entry nameiAPN1_CAEEL
    AccessioniPrimary (citable) accession number: Q10002
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: December 19, 2001
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3