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Q10002 (APN1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-(apurinic or apyrimidinic site) lyase

EC=4.2.99.18
Alternative name(s):
Apurinic-apyrimidinic endonuclease
Short name=AP endonuclease
Gene names
Name:apn-1
ORF Names:T05H10.2
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00152

Cofactor

Binds 3 zinc ions By similarity. HAMAP-Rule MF_00152

Subcellular location

Nucleus Potential HAMAP-Rule MF_00152.

Sequence similarities

Belongs to the AP endonuclease 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396DNA-(apurinic or apyrimidinic site) lyase HAMAP-Rule MF_00152
PRO_0000190899

Sites

Metal binding1851Zinc 1 By similarity
Metal binding2251Zinc 1 By similarity
Metal binding2611Zinc 1 By similarity
Metal binding2611Zinc 2 By similarity
Metal binding2951Zinc 2 By similarity
Metal binding2981Zinc 3 By similarity
Metal binding3321Zinc 2 By similarity
Metal binding3451Zinc 3 By similarity
Metal binding3471Zinc 3 By similarity
Metal binding3771Zinc 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q10002 [UniParc].

Last modified December 19, 2001. Version 2.
Checksum: 0C1D1B26AB35BA15

FASTA39644,747
        10         20         30         40         50         60 
MANKKVTFRE DVKSPAIRKL KQKLTPLKIK KGRGKIQKHI QKTLQKMKEE EESENQSPGT 

        70         80         90        100        110        120 
TVEETLTEEN ISTDKEETSK LENKPKKTRK TSGETIAQKK SRETVGVEVL KTSEGSSKML 

       130        140        150        160        170        180 
GFHVSAAGGL EQAIYNARAE GCRSFAMFVR NQRTWNHKPM SEEVVENWWK AVRETNFPLD 

       190        200        210        220        230        240 
QIVPHGSYLM NAGSPEAEKL EKSRLAMLDE CQRAEKLGIT MYNFHPGSTV GKCEKEECMT 

       250        260        270        280        290        300 
TIAETIDFVV EKTENIILVL ETMAGQGNSI GGTFEELKFI IDKVKVKSRV GVCIDTCHIF 

       310        320        330        340        350        360 
AGGYDIRTQK AYEEVMKNFG EVVGWNYLKA IHINDSKGDV GSKLDRHEHI GQGKIGKAAF 

       370        380        390 
ELLMNDNRLD GIPMILETPE GKYPEEMMIM YNMDKR 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[2]"The Caenorhabditis elegans gene CeAPN1 encodes a homolog of Escherichia coli and yeast apurinic/apyrimidinic endonuclease."
Masson J.Y., Tremblay S., Ramotar D.
Gene 179:291-293(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 104-396.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z47812 Genomic DNA. Translation: CAA87789.2.
U40707 mRNA. Translation: AAB39924.1.
PIRJC5235.
RefSeqNP_495687.1. NM_063286.5.
UniGeneCel.32771.

3D structure databases

ProteinModelPortalQ10002.
SMRQ10002. Positions 118-378.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6239.T05H10.2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaT05H10.2.1; T05H10.2.1; T05H10.2.
T05H10.2.2; T05H10.2.2; T05H10.2.
GeneID174291.
KEGGcel:CELE_T05H10.2.
UCSCT05H10.2. c. elegans.

Organism-specific databases

CTD174291.
WormBaseT05H10.2; CE28953; WBGene00000151; apn-1.

Phylogenomic databases

eggNOGCOG0648.
GeneTreeENSGT00390000013698.
HOGENOMHOG000224893.
InParanoidQ10002.
KOK10771.
OMAENWWKAV.

Family and domain databases

Gene3D3.20.20.150. 1 hit.
HAMAPMF_00152. Nfo.
InterProIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERPTHR21445. PTHR21445. 1 hit.
PfamPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMSSF51658. SSF51658. 1 hit.
TIGRFAMsTIGR00587. nfo. 1 hit.
PROSITEPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio883392.
PROQ10002.

Entry information

Entry nameAPN1_CAEEL
AccessionPrimary (citable) accession number: Q10002
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 19, 2001
Last modified: February 19, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase