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Q10002

- APN1_CAEEL

UniProt

Q10002 - APN1_CAEEL

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Protein

DNA-(apurinic or apyrimidinic site) lyase

Gene

apn-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Cofactori

Binds 3 zinc ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi185 – 1851Zinc 1By similarity
Metal bindingi225 – 2251Zinc 1By similarity
Metal bindingi261 – 2611Zinc 1By similarity
Metal bindingi261 – 2611Zinc 2By similarity
Metal bindingi295 – 2951Zinc 2By similarity
Metal bindingi298 – 2981Zinc 3By similarity
Metal bindingi332 – 3321Zinc 2By similarity
Metal bindingi345 – 3451Zinc 3By similarity
Metal bindingi347 – 3471Zinc 3By similarity
Metal bindingi377 – 3771Zinc 2By similarity

GO - Molecular functioni

  1. DNA-(apurinic or apyrimidinic site) lyase activity Source: WormBase
  2. DNA binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: WormBase
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. base-excision repair Source: WormBase
  2. DNA catabolic process, endonucleolytic Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
Alternative name(s):
Apurinic-apyrimidinic endonuclease
Short name:
AP endonuclease
Gene namesi
Name:apn-1
ORF Names:T05H10.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome II

Organism-specific databases

WormBaseiT05H10.2; CE28953; WBGene00000151; apn-1.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. mitochondrion Source: RefGenome
  2. nucleus Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396DNA-(apurinic or apyrimidinic site) lyasePRO_0000190899Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi6239.T05H10.2.

Structurei

3D structure databases

ProteinModelPortaliQ10002.
SMRiQ10002. Positions 118-378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.Curated

Phylogenomic databases

eggNOGiCOG0648.
GeneTreeiENSGT00390000013698.
HOGENOMiHOG000224893.
InParanoidiQ10002.
KOiK10771.
OMAiNTERKAD.
PhylomeDBiQ10002.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10002-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANKKVTFRE DVKSPAIRKL KQKLTPLKIK KGRGKIQKHI QKTLQKMKEE
60 70 80 90 100
EESENQSPGT TVEETLTEEN ISTDKEETSK LENKPKKTRK TSGETIAQKK
110 120 130 140 150
SRETVGVEVL KTSEGSSKML GFHVSAAGGL EQAIYNARAE GCRSFAMFVR
160 170 180 190 200
NQRTWNHKPM SEEVVENWWK AVRETNFPLD QIVPHGSYLM NAGSPEAEKL
210 220 230 240 250
EKSRLAMLDE CQRAEKLGIT MYNFHPGSTV GKCEKEECMT TIAETIDFVV
260 270 280 290 300
EKTENIILVL ETMAGQGNSI GGTFEELKFI IDKVKVKSRV GVCIDTCHIF
310 320 330 340 350
AGGYDIRTQK AYEEVMKNFG EVVGWNYLKA IHINDSKGDV GSKLDRHEHI
360 370 380 390
GQGKIGKAAF ELLMNDNRLD GIPMILETPE GKYPEEMMIM YNMDKR
Length:396
Mass (Da):44,747
Last modified:December 19, 2001 - v2
Checksum:i0C1D1B26AB35BA15
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z47812 Genomic DNA. Translation: CAA87789.2.
U40707 mRNA. Translation: AAB39924.1.
PIRiJC5235.
RefSeqiNP_495687.1. NM_063286.5.
UniGeneiCel.32771.

Genome annotation databases

EnsemblMetazoaiT05H10.2; T05H10.2; WBGene00000151.
GeneIDi174291.
KEGGicel:CELE_T05H10.2.
UCSCiT05H10.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z47812 Genomic DNA. Translation: CAA87789.2 .
U40707 mRNA. Translation: AAB39924.1 .
PIRi JC5235.
RefSeqi NP_495687.1. NM_063286.5.
UniGenei Cel.32771.

3D structure databases

ProteinModelPortali Q10002.
SMRi Q10002. Positions 118-378.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6239.T05H10.2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai T05H10.2 ; T05H10.2 ; WBGene00000151 .
GeneIDi 174291.
KEGGi cel:CELE_T05H10.2.
UCSCi T05H10.2. c. elegans.

Organism-specific databases

CTDi 174291.
WormBasei T05H10.2 ; CE28953 ; WBGene00000151 ; apn-1.

Phylogenomic databases

eggNOGi COG0648.
GeneTreei ENSGT00390000013698.
HOGENOMi HOG000224893.
InParanoidi Q10002.
KOi K10771.
OMAi NTERKAD.
PhylomeDBi Q10002.

Miscellaneous databases

NextBioi 883392.
PROi Q10002.

Family and domain databases

Gene3Di 3.20.20.150. 1 hit.
HAMAPi MF_00152. Nfo.
InterProi IPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view ]
PANTHERi PTHR21445. PTHR21445. 1 hit.
Pfami PF01261. AP_endonuc_2. 1 hit.
[Graphical view ]
SMARTi SM00518. AP2Ec. 1 hit.
[Graphical view ]
SUPFAMi SSF51658. SSF51658. 1 hit.
TIGRFAMsi TIGR00587. nfo. 1 hit.
PROSITEi PS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "The Caenorhabditis elegans gene CeAPN1 encodes a homolog of Escherichia coli and yeast apurinic/apyrimidinic endonuclease."
    Masson J.Y., Tremblay S., Ramotar D.
    Gene 179:291-293(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 104-396.

Entry informationi

Entry nameiAPN1_CAEEL
AccessioniPrimary (citable) accession number: Q10002
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 19, 2001
Last modified: October 1, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3