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Protein

DNA-(apurinic or apyrimidinic site) lyase

Gene

apn-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Cofactori

Zn2+By similarityNote: Binds 3 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi185 – 1851Zinc 1By similarity
Metal bindingi225 – 2251Zinc 1By similarity
Metal bindingi261 – 2611Zinc 1By similarity
Metal bindingi261 – 2611Zinc 2By similarity
Metal bindingi295 – 2951Zinc 2By similarity
Metal bindingi298 – 2981Zinc 3By similarity
Metal bindingi332 – 3321Zinc 2By similarity
Metal bindingi345 – 3451Zinc 3By similarity
Metal bindingi347 – 3471Zinc 3By similarity
Metal bindingi377 – 3771Zinc 2By similarity

GO - Molecular functioni

  • 3'-tyrosyl-DNA phosphodiesterase activity Source: CACAO
  • DNA-(apurinic or apyrimidinic site) lyase activity Source: WormBase
  • DNA binding Source: InterPro
  • double-stranded DNA 3'-5' exodeoxyribonuclease activity Source: CACAO
  • phosphoric diester hydrolase activity Source: WormBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • base-excision repair Source: WormBase
  • nucleic acid phosphodiester bond hydrolysis Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
Alternative name(s):
Apurinic-apyrimidinic endonuclease
Short name:
AP endonuclease
Gene namesi
Name:apn-1
ORF Names:T05H10.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiT05H10.2; CE28953; WBGene00000151; apn-1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396DNA-(apurinic or apyrimidinic site) lyasePRO_0000190899Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi6239.T05H10.2.1.

Structurei

3D structure databases

ProteinModelPortaliQ10002.
SMRiQ10002. Positions 118-378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.Curated

Phylogenomic databases

eggNOGiCOG0648.
GeneTreeiENSGT00390000013698.
HOGENOMiHOG000224893.
InParanoidiQ10002.
KOiK10771.
OMAiENWWKAV.
PhylomeDBiQ10002.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q10002-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANKKVTFRE DVKSPAIRKL KQKLTPLKIK KGRGKIQKHI QKTLQKMKEE
60 70 80 90 100
EESENQSPGT TVEETLTEEN ISTDKEETSK LENKPKKTRK TSGETIAQKK
110 120 130 140 150
SRETVGVEVL KTSEGSSKML GFHVSAAGGL EQAIYNARAE GCRSFAMFVR
160 170 180 190 200
NQRTWNHKPM SEEVVENWWK AVRETNFPLD QIVPHGSYLM NAGSPEAEKL
210 220 230 240 250
EKSRLAMLDE CQRAEKLGIT MYNFHPGSTV GKCEKEECMT TIAETIDFVV
260 270 280 290 300
EKTENIILVL ETMAGQGNSI GGTFEELKFI IDKVKVKSRV GVCIDTCHIF
310 320 330 340 350
AGGYDIRTQK AYEEVMKNFG EVVGWNYLKA IHINDSKGDV GSKLDRHEHI
360 370 380 390
GQGKIGKAAF ELLMNDNRLD GIPMILETPE GKYPEEMMIM YNMDKR
Length:396
Mass (Da):44,747
Last modified:December 19, 2001 - v2
Checksum:i0C1D1B26AB35BA15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47812 Genomic DNA. Translation: CAA87789.2.
U40707 mRNA. Translation: AAB39924.1.
PIRiJC5235.
RefSeqiNP_495687.1. NM_063286.5.
UniGeneiCel.32771.

Genome annotation databases

EnsemblMetazoaiT05H10.2; T05H10.2; WBGene00000151.
GeneIDi174291.
KEGGicel:CELE_T05H10.2.
UCSCiT05H10.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47812 Genomic DNA. Translation: CAA87789.2.
U40707 mRNA. Translation: AAB39924.1.
PIRiJC5235.
RefSeqiNP_495687.1. NM_063286.5.
UniGeneiCel.32771.

3D structure databases

ProteinModelPortaliQ10002.
SMRiQ10002. Positions 118-378.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.T05H10.2.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT05H10.2; T05H10.2; WBGene00000151.
GeneIDi174291.
KEGGicel:CELE_T05H10.2.
UCSCiT05H10.2. c. elegans.

Organism-specific databases

CTDi174291.
WormBaseiT05H10.2; CE28953; WBGene00000151; apn-1.

Phylogenomic databases

eggNOGiCOG0648.
GeneTreeiENSGT00390000013698.
HOGENOMiHOG000224893.
InParanoidiQ10002.
KOiK10771.
OMAiENWWKAV.
PhylomeDBiQ10002.

Miscellaneous databases

NextBioi883392.
PROiQ10002.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "The Caenorhabditis elegans gene CeAPN1 encodes a homolog of Escherichia coli and yeast apurinic/apyrimidinic endonuclease."
    Masson J.Y., Tremblay S., Ramotar D.
    Gene 179:291-293(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 104-396.

Entry informationi

Entry nameiAPN1_CAEEL
AccessioniPrimary (citable) accession number: Q10002
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 19, 2001
Last modified: June 24, 2015
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.