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Q0ZME8

- HEMA_CVHN5

UniProt

Q0ZME8 - HEMA_CVHN5

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Protein
Hemagglutinin-esterase
Gene
HE, 2
Organism
Human coronavirus HKU1 (isolate N5) (HCoV-HKU1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341Nucleophile By similarity
Active sitei205 – 2051Charge relay system By similarity
Active sitei302 – 3021Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2
OrganismiHuman coronavirus HKU1 (isolate N5) (HCoV-HKU1)
Taxonomic identifieri443241 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000001985: Genome

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host cell membrane; Single-pass type I membrane protein Reviewed prediction
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini14 – 360347Virion surface Reviewed prediction
Add
BLAST
Transmembranei361 – 38121Helical; Reviewed prediction
Add
BLAST
Topological domaini382 – 3854Intravirion Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1313 Reviewed prediction
Add
BLAST
Chaini14 – 385372Hemagglutinin-esterase
PRO_0000297763Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi38 ↔ 59 By similarity
Glycosylationi83 – 831N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi107 ↔ 155 By similarity
Glycosylationi110 – 1101N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi145 – 1451N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi171 – 1711N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi183 ↔ 249 By similarity
Disulfide bondi191 ↔ 222 By similarity
Glycosylationi196 – 1961N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi251 – 2511N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi280 ↔ 285 By similarity
Glycosylationi289 – 2891N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi317 – 3171N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi320 ↔ 344 By similarity
Glycosylationi331 – 3311N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

N-glycosylated in the RER By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ0ZME8.
SMRiQ0ZME8. Positions 15-348.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 121121Esterase domain first part By similarity
Add
BLAST
Regioni122 – 239118Receptor binding By similarity
Add
BLAST
Regioni240 – 352113Esterase domain second part By similarity
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0ZME8-1 [UniParc]FASTAAdd to Basket

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MLIIFLFFNF CYGFNEPLNV VSHLNHDWFL FGDSRSDCNH INNLKIKNYG    50
YLDIHPSLCN NGKISSSAGD SIFKSYHFTR FYNYTGEGDQ IIFYEGVNFS 100
PHHRFKCFFN GSNDVWIFNK VRFYRALYSN MALFRYLTFV DILYNFSFSI 150
KANICNSNIL SLNNPIFIST NYSKDVYFTL SGCSLYLVPL CLFKSNFSQY 200
YYNMDTGFAY GYSNFVSSDL DCTYISLKSG SYKIFSTGFV LSIPTKALCF 250
NKSKQFVPVQ VVDSRWNNLR ASDTSLSDAC QLPYCYFRNS SGNYVGKYDI 300
NHGDNGFTSI LSGLLYNVSC ISYYGSFLYD NFTSIWPRFS FGNCPTSAYI 350
KLNCFYDPLP IILQGILLFL ALLFIVFLLF LVYHG 385
Length:385
Mass (Da):44,429
Last modified:July 24, 2007 - v1
Checksum:i06FEAAB6C6F3B2B4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ339101 Genomic RNA. Translation: ABC70718.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ339101 Genomic RNA. Translation: ABC70718.1 .

3D structure databases

ProteinModelPortali Q0ZME8.
SMRi Q0ZME8. Positions 15-348.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative analysis of 22 coronavirus HKU1 genomes reveals a novel genotype and evidence of natural recombination in coronavirus HKU1."
    Woo P.C.Y., Lau S.K.P., Yip C.C.Y., Huang Y., Tsoi H.-W., Chan K.-H., Yuen K.-Y.
    J. Virol. 80:7136-7145(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_CVHN5
AccessioniPrimary (citable) accession number: Q0ZME8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: July 24, 2007
Last modified: April 16, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Isolate N5 belongs to genotype C. Genotype C probably arose from recombination between genotypes A and B.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi