Reviewed, UniProtKB/Swiss-Prot Q0ZME8 (HEMA_CVHN5)

Names and origin

Protein names

Recommended name:
    Hemagglutinin-esterase
      Short name=HE protein
    EC=3.1.1.53
Alternative name(s):
    E3 glycoprotein

Gene names

Name:	HE
ORF Names:	2

Organism

Human coronavirus HKU1 (isolate N5) (HCoV-HKU1) [Complete proteome]

Taxonomic identifier

443241 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Nidovirales › Coronaviridae › Coronavirus › Coronavirus group 2 › Coronavirus group 2a

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.
Catalytic activity	N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.
Subunit structure	Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE By similarity.
Subcellular location	Virion membrane; Single-pass type I membrane protein Potential. Host cell membrane; Single-pass type I membrane protein Potential. Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.
Post-translational modification	N-glycosylated in the RER By similarity.
Miscellaneous	Isolate N5 belongs to genotype C. Genotype C probably arose from recombination between genotypes A and B.
Sequence similarities	Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.

Ontologies

Keywords
Cellular component	Cell membrane Envelope protein Membrane Virion
Domain	Signal Transmembrane
Molecular function	Hemagglutinin Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	viral envelope fusion with host membrane Inferred from electronic annotation. Source: InterPro
Cellular component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	host cell surface receptor binding Inferred from electronic annotation. Source: InterPro sialate O-acetylesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 13	13	Potential
	Chain	14 – 385	372	Hemagglutinin-esterase		PRO_0000297763
Regions
	Topological domain	14 – 360	347	Extracellular Potential
	Transmembrane	361 – 381	21	Potential
	Topological domain	382 – 385	4	Cytoplasmic Potential
	Region	1 – 121	121	Esterase domain first part By similarity
	Region	122 – 239	118	Receptor binding By similarity
	Region	240 – 352	113	Esterase domain second part By similarity
Sites
	Active site	34	1	Nucleophile By similarity
	Active site	205	1	Charge relay system By similarity
	Active site	302	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	83	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	110	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	145	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	171	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	196	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	251	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	289	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	317	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	331	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0ZME8-1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 06FEAAB6C6F3B2B4	FASTA	385	44,429
	10 20 30 40 50 60 MLIIFLFFNF CYGFNEPLNV VSHLNHDWFL FGDSRSDCNH INNLKIKNYG YLDIHPSLCN 70 80 90 100 110 120 NGKISSSAGD SIFKSYHFTR FYNYTGEGDQ IIFYEGVNFS PHHRFKCFFN GSNDVWIFNK 130 140 150 160 170 180 VRFYRALYSN MALFRYLTFV DILYNFSFSI KANICNSNIL SLNNPIFIST NYSKDVYFTL 190 200 210 220 230 240 SGCSLYLVPL CLFKSNFSQY YYNMDTGFAY GYSNFVSSDL DCTYISLKSG SYKIFSTGFV 250 260 270 280 290 300 LSIPTKALCF NKSKQFVPVQ VVDSRWNNLR ASDTSLSDAC QLPYCYFRNS SGNYVGKYDI 310 320 330 340 350 360 NHGDNGFTSI LSGLLYNVSC ISYYGSFLYD NFTSIWPRFS FGNCPTSAYI KLNCFYDPLP 370 380 IILQGILLFL ALLFIVFLLF LVYHG

References

[1]

"Comparative analysis of 22 coronavirus HKU1 genomes reveals a novel genotype and evidence of natural recombination in coronavirus HKU1." Woo P.C.Y., Lau S.K.P., Yip C.C.Y., Huang Y., Tsoi H.-W., Chan K.-H., Yuen K.-Y. J. Virol. 80:7136-7145(2006) [PubMed: 16809319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ339101 Genomic RNA. Translation: ABC70718.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR007142. Hemagglutn-estrase_core. IPR003860. Hemagglutn-estrase_hemagglutn. [Graphical view]
Pfam	PF03996. Hema_esterase. 1 hit. PF02710. Hema_HEFG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMA_CVHN5

Accession

Primary (citable) accession number: Q0ZME8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	July 24, 2007
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)