Q0ZME8 (HEMA_CVHN5) Reviewed, UniProtKB/Swiss-Prot
Last modified April 16, 2014. Version 36. History...
Names and origin
|Protein names||Recommended name:|
Short name=HE protein
|Organism||Human coronavirus HKU1 (isolate N5) (HCoV-HKU1) [Complete proteome]|
|Taxonomic identifier||443241 [NCBI]|
|Taxonomic lineage||Viruses › ssRNA positive-strand viruses, no DNA stage › Nidovirales › Coronaviridae › Coronavirinae › Betacoronavirus ›|
|Virus host||Homo sapiens (Human) [TaxID: 9606]|
|Sequence length||385 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Inferred from homology|
General annotation (Comments)
Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.
N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.
Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE By similarity.
Virion membrane; Single-pass type I membrane protein Potential. Host cell membrane; Single-pass type I membrane protein Potential. Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.
N-glycosylated in the RER By similarity.
Isolate N5 belongs to genotype C. Genotype C probably arose from recombination between genotypes A and B.
Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.
|Cellular component||Host cell membrane|
Viral envelope protein
|Technical term||Complete proteome|
|Gene Ontology (GO)|
|Biological_process||fusion of virus membrane with host plasma membrane|
Inferred from electronic annotation. Source: InterPro
|Cellular_component||host cell plasma membrane|
Inferred from electronic annotation. Source: UniProtKB-SubCellintegral component of membrane
Inferred from electronic annotation. Source: UniProtKB-KWviral envelope
Inferred from electronic annotation. Source: UniProtKB-KWvirion membrane
Inferred from electronic annotation. Source: UniProtKB-SubCell
|Molecular_function||sialate O-acetylesterase activity|
Inferred from electronic annotation. Source: UniProtKB-EC
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Signal peptide||1 – 13||13||Potential|
|Chain||14 – 385||372||Hemagglutinin-esterase||PRO_0000297763|
|Topological domain||14 – 360||347||Virion surface Potential|
|Transmembrane||361 – 381||21||Helical; Potential|
|Topological domain||382 – 385||4||Intravirion Potential|
|Region||1 – 121||121||Esterase domain first part By similarity|
|Region||122 – 239||118||Receptor binding By similarity|
|Region||240 – 352||113||Esterase domain second part By similarity|
|Active site||34||1||Nucleophile By similarity|
|Active site||205||1||Charge relay system By similarity|
|Active site||302||1||Charge relay system By similarity|
Amino acid modifications
|Glycosylation||83||1||N-linked (GlcNAc...); by host Potential|
|Glycosylation||110||1||N-linked (GlcNAc...); by host Potential|
|Glycosylation||145||1||N-linked (GlcNAc...); by host Potential|
|Glycosylation||171||1||N-linked (GlcNAc...); by host Potential|
|Glycosylation||196||1||N-linked (GlcNAc...); by host Potential|
|Glycosylation||251||1||N-linked (GlcNAc...); by host Potential|
|Glycosylation||289||1||N-linked (GlcNAc...); by host Potential|
|Glycosylation||317||1||N-linked (GlcNAc...); by host Potential|
|Glycosylation||331||1||N-linked (GlcNAc...); by host Potential|
|Disulfide bond||38 ↔ 59||By similarity|
|Disulfide bond||107 ↔ 155||By similarity|
|Disulfide bond||183 ↔ 249||By similarity|
|Disulfide bond||191 ↔ 222||By similarity|
|Disulfide bond||280 ↔ 285||By similarity|
|Disulfide bond||320 ↔ 344||By similarity|
|||"Comparative analysis of 22 coronavirus HKU1 genomes reveals a novel genotype and evidence of natural recombination in coronavirus HKU1."|
Woo P.C.Y., Lau S.K.P., Yip C.C.Y., Huang Y., Tsoi H.-W., Chan K.-H., Yuen K.-Y.
J. Virol. 80:7136-7145(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
|DQ339101 Genomic RNA. Translation: ABC70718.1.|
3D structure databases
|SMR||Q0ZME8. Positions 15-348. |
Protocols and materials databases
Family and domain databases
|InterPro||IPR008980. Capsid_hemagglutn. |
|Pfam||PF03996. Hema_esterase. 1 hit. |
PF02710. Hema_HEFG. 1 hit.
|SUPFAM||SSF49818. SSF49818. 1 hit. |
|Accession||Primary (citable) accession number: Q0ZME8|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families