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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Human coronavirus HKU1 (isolate N5) (HCoV-HKU1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system (By similarity).By similarity

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei34NucleophileBy similarity1
Active sitei299Charge relay systemBy similarity1
Active sitei302Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2
OrganismiHuman coronavirus HKU1 (isolate N5) (HCoV-HKU1)
Taxonomic identifieri443241 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000001985 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini14 – 360Virion surfaceSequence analysisAdd BLAST347
Transmembranei361 – 381HelicalSequence analysisAdd BLAST21
Topological domaini382 – 385IntravirionSequence analysis4

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 13Sequence analysisAdd BLAST13
ChainiPRO_000029776314 – 385Hemagglutinin-esteraseAdd BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi38 ↔ 59By similarity
Glycosylationi83N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi107 ↔ 155By similarity
Glycosylationi110N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi145N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi171N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi183 ↔ 249By similarity
Disulfide bondi191 ↔ 222By similarity
Glycosylationi196N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi251N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi280 ↔ 285By similarity
Glycosylationi289N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi317N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi320 ↔ 344By similarity
Glycosylationi331N-linked (GlcNAc...); by hostSequence analysis1

Post-translational modificationi

N-glycosylated in the RER.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ0ZME8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 121Esterase domain first partBy similarityAdd BLAST121
Regioni122 – 239Receptor bindingBy similarityAdd BLAST118
Regioni240 – 352Esterase domain second partBy similarityAdd BLAST113

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0ZME8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIIFLFFNF CYGFNEPLNV VSHLNHDWFL FGDSRSDCNH INNLKIKNYG
60 70 80 90 100
YLDIHPSLCN NGKISSSAGD SIFKSYHFTR FYNYTGEGDQ IIFYEGVNFS
110 120 130 140 150
PHHRFKCFFN GSNDVWIFNK VRFYRALYSN MALFRYLTFV DILYNFSFSI
160 170 180 190 200
KANICNSNIL SLNNPIFIST NYSKDVYFTL SGCSLYLVPL CLFKSNFSQY
210 220 230 240 250
YYNMDTGFAY GYSNFVSSDL DCTYISLKSG SYKIFSTGFV LSIPTKALCF
260 270 280 290 300
NKSKQFVPVQ VVDSRWNNLR ASDTSLSDAC QLPYCYFRNS SGNYVGKYDI
310 320 330 340 350
NHGDNGFTSI LSGLLYNVSC ISYYGSFLYD NFTSIWPRFS FGNCPTSAYI
360 370 380
KLNCFYDPLP IILQGILLFL ALLFIVFLLF LVYHG
Length:385
Mass (Da):44,429
Last modified:July 24, 2007 - v1
Checksum:i06FEAAB6C6F3B2B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ339101 Genomic RNA. Translation: ABC70718.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ339101 Genomic RNA. Translation: ABC70718.1.

3D structure databases

ProteinModelPortaliQ0ZME8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiHEMA_CVHN5
AccessioniPrimary (citable) accession number: Q0ZME8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: July 24, 2007
Last modified: October 5, 2016
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Isolate N5 belongs to genotype C. Genotype C probably arose from recombination between genotypes A and B.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.