Q0WX57 (U17LO_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 17-like protein 24
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 17
Ubiquitin thioesterase 17
Ubiquitin-specific-processing protease 17

Gene names

Name:	USP17L24
Synonyms:	USP17, USP17H, USP17I, USP17J, USP17K, USP17L, USP17M
AND
Name:	USP17L25
AND
Name:	USP17L26
AND
Name:	USP17L27
AND
Name:	USP17L28
AND
Name:	USP17L29
AND
Name:	USP17L30

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes that may include cell proliferation, progression through the cell cycle, apoptosis, cell migration, and the cellular response to viral infection. Ref.1
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.1 Ref.2
Subcellular location	Nucleus › nucleolus. Endoplasmic reticulum By similarity Ref.2.
Tissue specificity	Expressed in heart, brain, liver and skeletal muscle. Ref.1
Sequence similarities	Belongs to the peptidase C19 family. USP17 subfamily.
Caution	The RS447 megasatellite DNA is a highly polymorphic conserved tandem repetitive sequence which contains a copy of the USP17 gene. It is present with an interindividual variation in copy number, ranging from 20 to 103, and can be found in the genome both on chromosome 4 and chromosome 8. The high similarity between the UPS17-like genes makes impossible to clearly assign data to one of the genes of the family. Oligonucleotides designed in RNAi experiments are for instance not specific of a given UPS17-like gene.

Ontologies

Keywords
Biological process	Apoptosis Ubl conjugation pathway
Cellular component	Endoplasmic reticulum Nucleus
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Ubiquitin carboxyl-terminal hydrolase 17-like protein 24		PRO_0000331643
Sites
	Active site	89	1	Nucleophile
	Active site	334	1	Proton acceptor By similarity
Experimental info
	Mutagenesis	89	1	C → S: Abolishes enzymatic activity. Loss of the pro-apoptotic function. Ref.1 Ref.2
	Sequence conflict	9	1	R → G in AAO38845. Ref.2
	Sequence conflict	9	1	R → G in AAQ11741. Ref.2
	Sequence conflict	9	1	R → G in AAQ11742. Ref.2
	Sequence conflict	23	1	S → P in AAQ11742. Ref.2
	Sequence conflict	262	1	P → S in AAQ11741. Ref.2
	Sequence conflict	310	1	P → Q in AAQ11741. Ref.2
	Sequence conflict	310	1	P → Q in AAQ11742. Ref.2
	Sequence conflict	360	1	T → I in AAO38845. Ref.2
	Sequence conflict	361	1	S → P in AAQ11742. Ref.2
	Sequence conflict	364	1	S → T in AAQ11742. Ref.2
	Sequence conflict	392	1	R → S in AAO38845. Ref.2
	Sequence conflict	398	1	D → A in AAQ11742. Ref.2
	Sequence conflict	430	1	Q → H in AAQ11742. Ref.2
	Sequence conflict	492	1	S → T in AAO38845. Ref.2
	Sequence conflict	492	1	S → T in AAQ11741. Ref.2
	Sequence conflict	492	1	S → T in AAQ11742. Ref.2
	Sequence conflict	511	1	R → G in AAQ11742. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q0WX57 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 5B0DC65280B2A098	FASTA	530	59,711
	10 20 30 40 50 60 MEDDSLYLRG EWQFNHFSKL TSSRPDAAFA EIQRTSLPEK SPLSCETRVD LCDDLAPVAR 70 80 90 100 110 120 QLAPREKLPL SSRRPAAVGA GLQNMGNTCY VNASLQCLTY TPPLANYMLS REHSQTCHRH 130 140 150 160 170 180 KGCMLCTMQA HITRALHNPG HVIQPSQALA AGFHRGKQED AHEFLMFTVD AMKKACLPGH 190 200 210 220 230 240 KQVDHHSKDT TLIHQIFGGY WRSQIKCLHC HGISDTFDPY LDIALDIQAA QSVQQALEQL 250 260 270 280 290 300 VKPEELNGEN AYHCGVCLQR APASKTLTLH TSAKVLILVL KRFSDVTGNK IAKNVQYPEC 310 320 330 340 350 360 LDMQPYMSQP NTGPLVYVLY AVLVHAGWSC HNGHYFSYVK AQEGQWYKMD DAEVTASSIT 370 380 390 400 410 420 SVLSQQAYVL FYIQKSEWER HSESVSRGRE PRALGAEDTD RRATQGELKR DHPCLQAPEL 430 440 450 460 470 480 DEHLVERATQ ESTLDHWKFL QEQNKTKPEF NVRKVEGTLP PDVLVIHQSK YKCGMKNHHP 490 500 510 520 530 EQQSSLLNLS SSTPTHQESM NTGTLASLRG RARRSKGKNK HSKRALLVCQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The RS447 human megasatellite tandem repetitive sequence encodes a novel deubiquitinating enzyme with a functional promoter." Saitoh Y., Miyamoto N., Okada T., Gondo Y., Showguchi-Miyata J., Hadano S., Ikeda J.-E. Genomics 67:291-300(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, MUTAGENESIS OF CYS-89.
[2]	"Hyaluronan- and RNA-binding deubiquitinating enzymes of USP17 family members associated with cell viability." Shin J.-M., Yoo K.-J., Kim M.-S., Kim D., Baek K.-H. BMC Genomics 7:292-292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE, SUBCELLULAR LOCATION, CATALYTIC ACTIVITY, MUTAGENESIS OF CYS-89.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Unstable transmission of the RS447 human megasatellite tandem repetitive sequence that contains the USP17 deubiquitinating enzyme gene." Okada T., Gondo Y., Goto J., Kanazawa I., Hadano S., Ikeda J.E. Hum. Genet. 110:302-313(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[5]	"The DUB/USP17 deubiquitinating enzymes, a multigene family within a tandemly repeated sequence." Burrows J.F., McGrattan M.J., Johnston J.A. Genomics 85:524-529(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, NOMENCLATURE.
[6]	"The DUB/USP17 deubiquitinating enzymes: a gene family within a tandemly repeated sequence, is also embedded within the copy number variable beta-defensin cluster." Burrows J.F., Scott C.J., Johnston J.A. BMC Genomics 11:250-250(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF544011 mRNA. Translation: AAQ11741.1. AF544012 mRNA. Translation: AAQ11742.1. AY188990 mRNA. Translation: AAO38845.1. AC116655 Genomic DNA. No translation available.
IPI	IPI00736155.
RefSeq	NP_001229255.1. NM_001242326.1. NP_001229256.1. NM_001242327.1. NP_001229257.1. NM_001242328.1. NP_001229259.1. NM_001242330.1. NP_001229260.1. NM_001242331.1. NP_001229261.1. NM_001242332.1. NP_001243796.1. NM_001256867.1.
UniGene	Hs.553810. Hs.631527. Hs.722783. Hs.723053. Hs.741133.
3D structure databases
ProteinModelPortal	Q0WX57.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q0WX57. 6 interactions.
Protein family/group databases
MEROPS	C19.023.
Proteomic databases
PaxDb	Q0WX57.
PRIDE	Q0WX57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000504104; ENSP00000422887; ENSG00000228856. ENST00000504481; ENSP00000425375; ENSG00000232264. ENST00000504543; ENSP00000423777; ENSG00000231051. ENST00000509271; ENSP00000422097; ENSG00000230430. ENST00000509660; ENSP00000427366; ENSG00000229579. ENST00000511681; ENSP00000422969; ENSG00000231637. ENST00000515574; ENSP00000423211; ENSG00000235780.
GeneID	728369. 728373. 728379. 728393. 728400. 728405. 728419.
KEGG	hsa:728369. hsa:728373. hsa:728379. hsa:728393. hsa:728400. hsa:728405. hsa:728419.
UCSC	uc021xll.1. human.
Organism-specific databases
CTD	728369. 728373. 728379. 728393. 728400. 728405. 728419.
GeneCards	GC04P009214. GC04P009360. GC04U901534.
H-InvDB	HIX0163954. HIX0163955.
HGNC	HGNC:44453. USP17L24. HGNC:44452. USP17L25. HGNC:44454. USP17L26. HGNC:44455. USP17L27. HGNC:44456. USP17L28. HGNC:44457. USP17L29. HGNC:44458. USP17L30.
HPA	HPA045642.
MIM	607011. gene.
neXtProt	NX_Q0WX57.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5533.
HOVERGEN	HBG007129.
InParanoid	Q0WX57.
KO	K11845.
OMA	DGENAYH.
OrthoDB	EOG42V8G0.
Gene expression databases
Genevestigator	Q0WX57.
Family and domain databases
InterPro	IPR006861. HABP4_PAIRBP1-bd. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF04774. HABP4_PAI-RBP1. 1 hit. PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	126977.
SOURCE	Search...

Entry information

Entry name

U17LO_HUMAN

Accession

Primary (citable) accession number: Q0WX57
Secondary accession number(s): A8MRA9, Q0WX56, Q3BEM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.