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Protein

Autophagy-related protein 3

Gene

ATG3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E2 conjugating enzyme responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. This step is required for the membrane association of ATG8. The formation of the ATG8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei258Glycyl thioester intermediateSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy, Protein transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-1632852 Macroautophagy

Protein family/group databases

TCDBi9.A.15.3.1 the autophagy-related phagophore-formation transporter (apt) family

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 3
Alternative name(s):
Autophagy-related E2-like conjugation enzyme ATG3
Short name:
AtAPG3
Short name:
Protein autophagy 3
Gene namesi
Name:ATG3
Synonyms:APG3
Ordered Locus Names:At5g61500
ORF Names:K11J9.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G61500
TAIRilocus:2151566 AT5G61500

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002869371 – 313Autophagy-related protein 3Add BLAST313

Proteomic databases

PaxDbiQ0WWQ1
PRIDEiQ0WWQ1

Expressioni

Gene expression databases

ExpressionAtlasiQ0WWQ1 baseline and differential
GenevisibleiQ0WWQ1 AT

Interactioni

Subunit structurei

Interacts with ATG8 through an intermediate thioester bond between Cys-258 and the C-terminal Gly of ATG8. Also interacts with the C-terminal region of the E1-like ATG7 enzyme (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG12BQ9LVK32EBI-8276607,EBI-8276588

Protein-protein interaction databases

BioGridi21515, 3 interactors
IntActiQ0WWQ1, 1 interactor
MINTiQ0WWQ1
STRINGi3702.AT5G61500.1

Structurei

Secondary structure

1313
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi26 – 30Combined sources5
Helixi35 – 48Combined sources14
Beta strandi53 – 55Combined sources3
Helixi59 – 61Combined sources3
Beta strandi71 – 80Combined sources10
Beta strandi193 – 202Combined sources10
Turni203 – 206Combined sources4
Beta strandi207 – 215Combined sources9
Helixi224 – 229Combined sources6
Beta strandi240 – 244Combined sources5
Beta strandi246 – 255Combined sources10
Helixi257 – 259Combined sources3
Helixi260 – 274Combined sources15
Helixi280 – 282Combined sources3
Helixi283 – 291Combined sources9
Turni292 – 294Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VX8X-ray3.11B/C26-313[»]
ProteinModelPortaliQ0WWQ1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG3 family.Curated

Phylogenomic databases

eggNOGiKOG2981 Eukaryota
ENOG410Y3BC LUCA
HOGENOMiHOG000234613
KOiK08343
OMAiYEDVSQD
OrthoDBiEOG09360BR2
PhylomeDBiQ0WWQ1

Family and domain databases

InterProiView protein in InterPro
IPR007135 Autophagy-rel_prot_3
IPR019461 Autophagy-rel_prot_3_C
IPR007134 Autophagy-rel_prot_3_N
PfamiView protein in Pfam
PF03987 Autophagy_act_C, 1 hit
PF10381 Autophagy_C, 1 hit
PF03986 Autophagy_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q0WWQ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLSQKLHEA FKGTVERITG PRTISAFKEK GVLSVSEFVL AGDNLVSKCP
60 70 80 90 100
TWSWESGDAS KRKPYLPSDK QFLITRNVPC LRRAASVAED YEAAGGEVLV
110 120 130 140 150
DDEDNDGWLA THGKPKDKGK EEDNLPSMDA LDINEKNTIQ SIPTYFGGEE
160 170 180 190 200
DDDIPDMEEF DEADNVVEND PATLQSTYLV AHEPDDDNIL RTRTYDLSIT
210 220 230 240 250
YDKYYQTPRV WLTGYDESRM LLQPELVMED VSQDHARKTV TIEDHPHLPG
260 270 280 290 300
KHASVHPCRH GAVMKKIIDV LMSRGVEPEV DKYLFLFLKF MASVIPTIEY
310
DYTMDFDLGS SST
Length:313
Mass (Da):35,332
Last modified:May 15, 2007 - v2
Checksum:iD6357AE8646FEC2E
GO

Sequence cautioni

The sequence BAB08995 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203K → E in BAE98447 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073170 mRNA Translation: BAB88382.1
AB012239 Genomic DNA Translation: BAB08995.1 Sequence problems.
CP002688 Genomic DNA Translation: AED97476.1
BT024713 mRNA Translation: ABD59051.1
AK226290 mRNA Translation: BAE98447.1
AY088587 mRNA Translation: AAM66117.1
RefSeqiNP_568934.1, NM_125543.5
UniGeneiAt.22151

Genome annotation databases

EnsemblPlantsiAT5G61500.1; AT5G61500.1; AT5G61500
GeneIDi836271
GrameneiAT5G61500.1; AT5G61500.1; AT5G61500
KEGGiath:AT5G61500

Similar proteinsi

Entry informationi

Entry nameiATG3_ARATH
AccessioniPrimary (citable) accession number: Q0WWQ1
Secondary accession number(s): Q8L982, Q8S930, Q9FKG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: May 23, 2018
This is version 79 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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