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Protein

Autophagy-related protein 3

Gene

ATG3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

E2 conjugating enzyme responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. This step is required for the membrane association of ATG8. The formation of the ATG8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei258 – 2581Glycyl thioester intermediateSequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 3
Alternative name(s):
Autophagy-related E2-like conjugation enzyme ATG3
Short name:
AtAPG3
Short name:
Protein autophagy 3
Gene namesi
Name:ATG3
Synonyms:APG3
Ordered Locus Names:At5g61500
ORF Names:K11J9.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G61500.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic ubiquitin ligase complex Source: GO_Central
  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Autophagy-related protein 3PRO_0000286937Add
BLAST

Proteomic databases

PaxDbiQ0WWQ1.
PRIDEiQ0WWQ1.

Interactioni

Subunit structurei

Interacts with ATG8 through an intermediate thioester bond between Cys-258 and the C-terminal Gly of ATG8. Also interacts with the C-terminal region of the E1-like ATG7 enzyme (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG12BQ9LVK32EBI-8276607,EBI-8276588

Protein-protein interaction databases

BioGridi21515. 3 interactions.
IntActiQ0WWQ1. 1 interaction.
STRINGi3702.AT5G61500.1.

Structurei

Secondary structure

1
313
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi26 – 305Combined sources
Helixi35 – 4814Combined sources
Beta strandi53 – 553Combined sources
Helixi59 – 613Combined sources
Beta strandi71 – 8010Combined sources
Beta strandi193 – 20210Combined sources
Turni203 – 2064Combined sources
Beta strandi207 – 2159Combined sources
Helixi224 – 2296Combined sources
Beta strandi240 – 2445Combined sources
Beta strandi246 – 25510Combined sources
Helixi257 – 2593Combined sources
Helixi260 – 27415Combined sources
Helixi280 – 2823Combined sources
Helixi283 – 2919Combined sources
Turni292 – 2943Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VX8X-ray3.11B/C26-313[»]
ProteinModelPortaliQ0WWQ1.
SMRiQ0WWQ1. Positions 26-87, 143-304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG3 family.Curated

Phylogenomic databases

eggNOGiNOG237080.
HOGENOMiHOG000234613.
KOiK08343.
OMAiKYDMYIT.
PhylomeDBiQ0WWQ1.

Family and domain databases

InterProiIPR007135. Autophagy-rel_prot_3.
IPR019461. Autophagy-rel_prot_3_C.
IPR007134. Autophagy-rel_prot_3_N.
[Graphical view]
PfamiPF03987. Autophagy_act_C. 1 hit.
PF10381. Autophagy_Cterm. 1 hit.
PF03986. Autophagy_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0WWQ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLSQKLHEA FKGTVERITG PRTISAFKEK GVLSVSEFVL AGDNLVSKCP
60 70 80 90 100
TWSWESGDAS KRKPYLPSDK QFLITRNVPC LRRAASVAED YEAAGGEVLV
110 120 130 140 150
DDEDNDGWLA THGKPKDKGK EEDNLPSMDA LDINEKNTIQ SIPTYFGGEE
160 170 180 190 200
DDDIPDMEEF DEADNVVEND PATLQSTYLV AHEPDDDNIL RTRTYDLSIT
210 220 230 240 250
YDKYYQTPRV WLTGYDESRM LLQPELVMED VSQDHARKTV TIEDHPHLPG
260 270 280 290 300
KHASVHPCRH GAVMKKIIDV LMSRGVEPEV DKYLFLFLKF MASVIPTIEY
310
DYTMDFDLGS SST
Length:313
Mass (Da):35,332
Last modified:May 15, 2007 - v2
Checksum:iD6357AE8646FEC2E
GO

Sequence cautioni

The sequence BAB08995.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti203 – 2031K → E in BAE98447 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073170 mRNA. Translation: BAB88382.1.
AB012239 Genomic DNA. Translation: BAB08995.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97476.1.
BT024713 mRNA. Translation: ABD59051.1.
AK226290 mRNA. Translation: BAE98447.1.
AY088587 mRNA. Translation: AAM66117.1.
RefSeqiNP_568934.1. NM_125543.4.
UniGeneiAt.22151.

Genome annotation databases

EnsemblPlantsiAT5G61500.1; AT5G61500.1; AT5G61500.
GeneIDi836271.
KEGGiath:AT5G61500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073170 mRNA. Translation: BAB88382.1.
AB012239 Genomic DNA. Translation: BAB08995.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97476.1.
BT024713 mRNA. Translation: ABD59051.1.
AK226290 mRNA. Translation: BAE98447.1.
AY088587 mRNA. Translation: AAM66117.1.
RefSeqiNP_568934.1. NM_125543.4.
UniGeneiAt.22151.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VX8X-ray3.11B/C26-313[»]
ProteinModelPortaliQ0WWQ1.
SMRiQ0WWQ1. Positions 26-87, 143-304.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21515. 3 interactions.
IntActiQ0WWQ1. 1 interaction.
STRINGi3702.AT5G61500.1.

Proteomic databases

PaxDbiQ0WWQ1.
PRIDEiQ0WWQ1.

Protocols and materials databases

DNASUi836271.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G61500.1; AT5G61500.1; AT5G61500.
GeneIDi836271.
KEGGiath:AT5G61500.

Organism-specific databases

TAIRiAT5G61500.

Phylogenomic databases

eggNOGiNOG237080.
HOGENOMiHOG000234613.
KOiK08343.
OMAiKYDMYIT.
PhylomeDBiQ0WWQ1.

Miscellaneous databases

PROiQ0WWQ1.

Family and domain databases

InterProiIPR007135. Autophagy-rel_prot_3.
IPR019461. Autophagy-rel_prot_3_C.
IPR007134. Autophagy-rel_prot_3_N.
[Graphical view]
PfamiPF03987. Autophagy_act_C. 1 hit.
PF10381. Autophagy_Cterm. 1 hit.
PF03986. Autophagy_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Leaf senescence and starvation-induced chlorosis are accelerated by the disruption of an Arabidopsis autophagy gene."
    Hanaoka H., Noda T., Shirano Y., Kato T., Hayashi H., Shibata D., Tabata S., Ohsumi Y.
    Plant Physiol. 129:1181-1193(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE, GENE FAMILY.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Shinn P., Chen H., Kim C.J., Ecker J.R.
    Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiATG3_ARATH
AccessioniPrimary (citable) accession number: Q0WWQ1
Secondary accession number(s): Q8L982, Q8S930, Q9FKG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: July 22, 2015
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.