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Q0WWQ1 (ATG3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Autophagy-related protein 3
Alternative name(s):
Autophagy-related E2-like conjugation enzyme ATG3
Short name=AtAPG3
Short name=Protein autophagy 3
Gene names
Name:ATG3
Synonyms:APG3
Ordered Locus Names:At5g61500
ORF Names:K11J9.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

E2 conjugating enzyme responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. This step is required for the membrane association of ATG8. The formation of the ATG8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt).

Subunit structure

Interacts with ATG8 through an intermediate thioester bond between Cys-258 and the C-terminal Gly of ATG8. Also interacts with the C-terminal region of the E1-like ATG7 enzyme By similarity.

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the ATG3 family.

Sequence caution

The sequence BAB08995.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processAutophagy
Protein transport
Transport
Ubl conjugation pathway
   Cellular componentCytoplasm
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processautophagy

Inferred from electronic annotation. Source: UniProtKB-KW

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytosol

Inferred from direct assay PubMed 21166475. Source: TAIR

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 23238393. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ATG12BQ9LVK32EBI-8276607,EBI-8276588

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Autophagy-related protein 3
PRO_0000286937

Sites

Active site2581Glycyl thioester intermediate Potential

Experimental info

Sequence conflict2031K → E in BAE98447. Ref.5

Secondary structure

............................ 313
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q0WWQ1 [UniParc].

Last modified May 15, 2007. Version 2.
Checksum: D6357AE8646FEC2E

FASTA31335,332
        10         20         30         40         50         60 
MVLSQKLHEA FKGTVERITG PRTISAFKEK GVLSVSEFVL AGDNLVSKCP TWSWESGDAS 

        70         80         90        100        110        120 
KRKPYLPSDK QFLITRNVPC LRRAASVAED YEAAGGEVLV DDEDNDGWLA THGKPKDKGK 

       130        140        150        160        170        180 
EEDNLPSMDA LDINEKNTIQ SIPTYFGGEE DDDIPDMEEF DEADNVVEND PATLQSTYLV 

       190        200        210        220        230        240 
AHEPDDDNIL RTRTYDLSIT YDKYYQTPRV WLTGYDESRM LLQPELVMED VSQDHARKTV 

       250        260        270        280        290        300 
TIEDHPHLPG KHASVHPCRH GAVMKKIIDV LMSRGVEPEV DKYLFLFLKF MASVIPTIEY 

       310 
DYTMDFDLGS SST 

« Hide

References

« Hide 'large scale' references
[1]"Leaf senescence and starvation-induced chlorosis are accelerated by the disruption of an Arabidopsis autophagy gene."
Hanaoka H., Noda T., Shirano Y., Kato T., Hayashi H., Shibata D., Tabata S., Ohsumi Y.
Plant Physiol. 129:1181-1193(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE, GENE FAMILY.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Shinn P., Chen H., Kim C.J., Ecker J.R.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB073170 mRNA. Translation: BAB88382.1.
AB012239 Genomic DNA. Translation: BAB08995.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97476.1.
BT024713 mRNA. Translation: ABD59051.1.
AK226290 mRNA. Translation: BAE98447.1.
AY088587 mRNA. Translation: AAM66117.1.
RefSeqNP_568934.1. NM_125543.4.
UniGeneAt.22151.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3VX8X-ray3.11B/C26-313[»]
ProteinModelPortalQ0WWQ1.
SMRQ0WWQ1. Positions 26-87, 143-304.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid21515. 3 interactions.
IntActQ0WWQ1. 1 interaction.

Proteomic databases

PaxDbQ0WWQ1.
PRIDEQ0WWQ1.

Protocols and materials databases

DNASU836271.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G61500.1; AT5G61500.1; AT5G61500.
GeneID836271.
KEGGath:AT5G61500.

Organism-specific databases

TAIRAT5G61500.

Phylogenomic databases

eggNOGNOG237080.
HOGENOMHOG000234613.
InParanoidQ0WWQ1.
KOK08343.
OMAASVAEDY.
PhylomeDBQ0WWQ1.

Gene expression databases

GenevestigatorQ0WWQ1.

Family and domain databases

InterProIPR007135. Autophagy-rel_prot_3.
IPR019461. Autophagy-rel_prot_3_C.
IPR007134. Autophagy-rel_prot_3_N.
[Graphical view]
PfamPF03987. Autophagy_act_C. 1 hit.
PF10381. Autophagy_Cterm. 1 hit.
PF03986. Autophagy_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ0WWQ1.

Entry information

Entry nameATG3_ARATH
AccessionPrimary (citable) accession number: Q0WWQ1
Secondary accession number(s): Q8L982, Q8S930, Q9FKG9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: June 11, 2014
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names