Q0WWQ1 (ATG3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Autophagy-related protein 3

Alternative name(s):
Autophagy-related E2-like conjugation enzyme ATG3
Short name=AtAPG3
Short name=Protein autophagy 3

Gene names

Name:	ATG3
Synonyms:	APG3
Ordered Locus Names:	At5g61500
ORF Names:	K11J9.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E2 conjugating enzyme responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. This step is required for the membrane association of ATG8. The formation of the ATG8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt).
Subunit structure	Interacts with ATG8 through an intermediate thioester bond between Cys-258 and the C-terminal Gly of ATG8. Also interacts with the C-terminal region of the E1-like ATG7 enzyme By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the ATG3 family.
Sequence caution	The sequence BAB08995.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport Ubl conjugation pathway
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from direct assay PubMed 21166475. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 313

313

Autophagy-related protein 3

PRO_0000286937

Sites

Active site

258

1

Glycyl thioester intermediate Potential

Experimental info

Sequence conflict

203

1

K → E in BAE98447. Ref.5

Secondary structure

1

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313

Helix Strand Turn

Details...

Helix

26 – 30

5

Helix

35 – 48

14

Beta strand

53 – 55

3

Helix

59 – 61

3

Beta strand

71 – 80

10

Beta strand

193 – 202

10

Turn

203 – 206

4

Beta strand

207 – 215

9

Helix

224 – 229

6

Beta strand

240 – 244

5

Beta strand

246 – 255

10

Helix

257 – 259

3

Helix

260 – 274

15

Helix

280 – 282

3

Helix

283 – 291

9

Turn

292 – 294

3

Sequences

	Sequence		Length	Mass (Da)
	Q0WWQ1 [UniParc]. Last modified May 15, 2007. Version 2. Checksum: D6357AE8646FEC2E	FASTA	313	35,332
	10 20 30 40 50 60 MVLSQKLHEA FKGTVERITG PRTISAFKEK GVLSVSEFVL AGDNLVSKCP TWSWESGDAS 70 80 90 100 110 120 KRKPYLPSDK QFLITRNVPC LRRAASVAED YEAAGGEVLV DDEDNDGWLA THGKPKDKGK 130 140 150 160 170 180 EEDNLPSMDA LDINEKNTIQ SIPTYFGGEE DDDIPDMEEF DEADNVVEND PATLQSTYLV 190 200 210 220 230 240 AHEPDDDNIL RTRTYDLSIT YDKYYQTPRV WLTGYDESRM LLQPELVMED VSQDHARKTV 250 260 270 280 290 300 TIEDHPHLPG KHASVHPCRH GAVMKKIIDV LMSRGVEPEV DKYLFLFLKF MASVIPTIEY 310 DYTMDFDLGS SST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Leaf senescence and starvation-induced chlorosis are accelerated by the disruption of an Arabidopsis autophagy gene." Hanaoka H., Noda T., Shirano Y., Kato T., Hayashi H., Shibata D., Tabata S., Ohsumi Y. Plant Physiol. 129:1181-1193(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE, GENE FAMILY.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Shinn P., Chen H., Kim C.J., Ecker J.R. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB073170 mRNA. Translation: BAB88382.1.
AB012239 Genomic DNA. Translation: BAB08995.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97476.1.
BT024713 mRNA. Translation: ABD59051.1.
AK226290 mRNA. Translation: BAE98447.1.
AY088587 mRNA. Translation: AAM66117.1.

IPI

IPI00521348.

RefSeq

NP_568934.1. NM_125543.4.

UniGene

At.22151.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3VX8	X-ray	3.11	B/C	26-313	[»]

ProteinModelPortal

Q0WWQ1.

SMR

Q0WWQ1. Positions 26-304.

ModBase

Search...

Proteomic databases

PaxDb

Q0WWQ1.

PRIDE

Q0WWQ1.

Protocols and materials databases

DNASU

836271.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT5G61500.1; AT5G61500.1; AT5G61500.

GeneID

836271.

KEGG

ath:AT5G61500.

Organism-specific databases

TAIR

At5g61500.

Phylogenomic databases

eggNOG

NOG237080.

HOGENOM

HOG000234613.

InParanoid

Q0WWQ1.

KO

K08343.

OMA

MLLQPEL.

PhylomeDB

Q0WWQ1.

ProtClustDB

CLSN2690060.

Gene expression databases

ArrayExpress

Q0WWQ1.

Genevestigator

Q0WWQ1.

Family and domain databases

InterPro

IPR007135. Autophagy-rel_prot_3.
IPR019461. Autophagy-rel_prot_3_C.
IPR007134. Autophagy-rel_prot_3_N.
[Graphical view]

Pfam

PF03987. Autophagy_act_C. 1 hit.
PF10381. Autophagy_Cterm. 1 hit.
PF03986. Autophagy_N. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ATG3_ARATH

Accession

Primary (citable) accession number: Q0WWQ1
Secondary accession number(s): Q8L982, Q8S930, Q9FKG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program