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Protein

GDSL esterase/lipase EXL4

Gene

EXL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the formation of pollen coats and male fertility.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei35NucleophileBy similarity1
Active sitei318By similarity1
Active sitei321By similarity1

GO - Molecular functioni

  • lipase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT1G75910-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
GDSL esterase/lipase EXL4 (EC:3.1.1.-)
Alternative name(s):
Family II extracellular lipase 4
Short name:
Family II lipase EXL4
Gene namesi
Name:EXL4
Ordered Locus Names:At1g75910
ORF Names:T4O12.250
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G75910
TAIRilocus:2204410 AT1G75910

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

Reduced pollen fertility. Pollen grain exhibit a partial formation of coat and impaired water absorption and germination capacities.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000036733122 – 343GDSL esterase/lipase EXL4Add BLAST322

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi23N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ0WUV7

Expressioni

Tissue specificityi

Flower buds and pollen.1 Publication

Developmental stagei

Strongly expressed in tapetal cells at the flower developmental stage 10 to middle 12, where the components of pollen coat are synthesized actively.1 Publication

Gene expression databases

ExpressionAtlasiQ0WUV7 baseline and differential
GenevisibleiQ0WUV7 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G75910.1

Structurei

3D structure databases

ProteinModelPortaliQ0WUV7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 'GDSL' lipolytic enzyme family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JWV9 Eukaryota
ENOG41104JW LUCA
HOGENOMiHOG000237650
InParanoidiQ0WUV7
OMAiRGAKFVY
OrthoDBiEOG0936150F
PhylomeDBiQ0WUV7

Family and domain databases

CDDicd01837 SGNH_plant_lipase_like, 1 hit
Gene3Di3.40.50.1110, 1 hit
InterProiView protein in InterPro
IPR001087 GDSL
IPR008265 Lipase_GDSL_AS
IPR036514 SGNH_hydro_sf
IPR035669 SGNH_plant_lipase_like
PfamiView protein in Pfam
PF00657 Lipase_GDSL, 1 hit
PROSITEiView protein in PROSITE
PS01098 LIPASE_GDSL_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0WUV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCSKITLVLT LFSSYFISTD AVNGSFPALL AFGDSILDTG NNNFLLTFMK
60 70 80 90 100
GNIWPYGRSF SMRRATGRFG NGRVFSDIVA EGLGIKKILP AYRKLFNSPS
110 120 130 140 150
DLRTGVCFAS GGAGVDPVTS KLLRVLTPKD QVNDFKGYIR KLKATAGPSR
160 170 180 190 200
ASSIVSNAVI LVSQGNNDIG ISYFGTPTAA FRGLTPNRYT TKLAGWNKQF
210 220 230 240 250
MKELYDQGAR KFAVMGVIPL GCLPMTRIFL GGFVITCNFF ANRVAEQYNG
260 270 280 290 300
KLRSGTKSWG REAGFRGAKF VYVDMYNTLM DVIKNYRRYG FSNEKNGCCC
310 320 330 340
MITAIIPCPN PDKYVFYDFV HPSEKAYRTI SKKLVQDIKN GLA
Length:343
Mass (Da):37,911
Last modified:September 5, 2006 - v1
Checksum:i66FA2E7D1105936C
GO

Sequence cautioni

The sequence AAF79815 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At1g75900 has been split into 2 genes: At1g75900 and At1g75910.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21 – 23Missing in AAK30019 (PubMed:11431566).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028612 mRNA Translation: AAK30019.1
AC007396 Genomic DNA Translation: AAF79815.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE35773.1
AK227028 mRNA Translation: BAE99091.1
PIRiA96788
RefSeqiNP_177719.1, NM_106241.3
UniGeneiAt.25298

Genome annotation databases

EnsemblPlantsiAT1G75910.1; AT1G75910.1; AT1G75910
GeneIDi843924
GrameneiAT1G75910.1; AT1G75910.1; AT1G75910
KEGGiath:AT1G75910

Similar proteinsi

Entry informationi

Entry nameiEXL4_ARATH
AccessioniPrimary (citable) accession number: Q0WUV7
Secondary accession number(s): Q93X95, Q9LQS5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 5, 2006
Last modified: April 25, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health