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Protein

Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 8

Gene

TPS8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

GO - Molecular functioni

  1. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

GO - Biological processi

  1. trehalose biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT1G70290-MONOMER.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 8 (EC:2.4.1.15)
Alternative name(s):
Trehalose-6-phosphate synthase 8
Short name:
AtTPS8
Gene namesi
Name:TPS8
Synonyms:TPS2, TPSC
Ordered Locus Names:At1g70290
ORF Names:F17O7.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G70290.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 856856Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 8PRO_0000324829Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51PhosphoserineBy similarity
Modified residuei32 – 321PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ0WUI9.
PRIDEiQ0WUI9.

Expressioni

Tissue specificityi

Expressed in leaves, roots, stems and flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ0WUI9. baseline and differential.
GenevestigatoriQ0WUI9.

Interactioni

Protein-protein interaction databases

BioGridi28585. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ0WUI9.
SMRiQ0WUI9. Positions 113-541.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 541485GlycosyltransferaseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi837 – 8404Poly-Ser

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 20 family.Curated
In the C-terminal section; belongs to the trehalose phosphatase family.Curated

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191476.
InParanoidiQ0WUI9.
KOiK16055.
OMAiENASSIC.
PhylomeDBiQ0WUI9.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR003337. Trehalose_PPase.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
PF02358. Trehalose_PPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.
TIGR00685. T6PP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0WUI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSRSCANFL DLSSWDLLDF PQTPRTLPRV MTVPGIITDV DGDTTSEVTS
60 70 80 90 100
TSGGSRERKI IVANMLPLQS KRDAETGKWC FNWDEDSLQL QLRDGFSSET
110 120 130 140 150
EFLYVGSLNV DIETNEQEEV SQKLLEEFNC VATFLSQELQ EMFYLGFCKH
160 170 180 190 200
QLWPLFHYML PMFPDHGDRF DRRLWQAYVS ANKIFSDRVM EVINPEDDYV
210 220 230 240 250
WIQDYHLMVL PTFLRKRFNR IKLGFFLHSP FPSSEIYRTL PVRDEILRGL
260 270 280 290 300
LNCDLIGFHT FDYARHFLSC CSRMLGLDYE SKRGHIGLDY FGRTVYIKIL
310 320 330 340 350
PVGVHMGRLE SVLSLDSTAA KTKEIQEQFK GKKLVLGIDD MDIFKGISLK
360 370 380 390 400
LIAMEHLFET YWHLKGKVVL VQIVNPARSS GKDVEEAKRE TYETARRINE
410 420 430 440 450
RYGTSDYKPI VLIDRLVPRS EKTAYYAAAD CCLVNAVRDG MNLVPYKYIV
460 470 480 490 500
CRQGTRSNKA VVDSSPRTST LVVSEFIGCS PSLSGAIRVN PWDVDAVAEA
510 520 530 540 550
VNSALKMSET EKQLRHEKHY HYISTHDVGY WAKSFMQDLE RACRDHYSKR
560 570 580 590 600
CWGIGFGLGF RVLSLSPSFR KLSVEHIVPV YRKTQRRAIF LDYDGTLVPE
610 620 630 640 650
SSIVQDPSNE VVSVLKALCE DPNNTVFIVS GRGRESLSNW LSPCENLGIA
660 670 680 690 700
AEHGYFIRWK SKDEWETCYS PTDTEWRSMV EPVMRSYMEA TDGTSIEFKE
710 720 730 740 750
SALVWHHQDA DPDFGSCQAK EMLDHLESVL ANEPVVVKRG QHIVEVKPQG
760 770 780 790 800
VSKGLAAEKV IREMVERGEP PEMVMCIGDD RSDEDMFESI LSTVTNPELL
810 820 830 840 850
VQPEVFACTV GRKPSKAKYF LDDEADVLKL LRGLGDSSSS LKPSSSHTQV

AFESIV
Length:856
Mass (Da):97,561
Last modified:September 5, 2006 - v1
Checksum:i7AA5627BF8307A7C
GO

Sequence cautioni

The sequence AAC18810.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003671 Genomic DNA. Translation: AAC18810.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE35042.1.
AK227167 mRNA. Translation: BAE99209.1.
AF155150 mRNA. Translation: AAO15311.1.
PIRiT01494.
RefSeqiNP_177186.2. NM_105697.3.
UniGeneiAt.27738.

Genome annotation databases

EnsemblPlantsiAT1G70290.1; AT1G70290.1; AT1G70290.
GeneIDi843365.
KEGGiath:AT1G70290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003671 Genomic DNA. Translation: AAC18810.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE35042.1.
AK227167 mRNA. Translation: BAE99209.1.
AF155150 mRNA. Translation: AAO15311.1.
PIRiT01494.
RefSeqiNP_177186.2. NM_105697.3.
UniGeneiAt.27738.

3D structure databases

ProteinModelPortaliQ0WUI9.
SMRiQ0WUI9. Positions 113-541.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28585. 1 interaction.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Proteomic databases

PaxDbiQ0WUI9.
PRIDEiQ0WUI9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G70290.1; AT1G70290.1; AT1G70290.
GeneIDi843365.
KEGGiath:AT1G70290.

Organism-specific databases

TAIRiAT1G70290.

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191476.
InParanoidiQ0WUI9.
KOiK16055.
OMAiENASSIC.
PhylomeDBiQ0WUI9.

Enzyme and pathway databases

BioCyciARA:AT1G70290-MONOMER.

Miscellaneous databases

PROiQ0WUI9.

Gene expression databases

ExpressionAtlasiQ0WUI9. baseline and differential.
GenevestigatoriQ0WUI9.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR003337. Trehalose_PPase.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
PF02358. Trehalose_PPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.
TIGR00685. T6PP. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Trehalose metabolism in Arabidopsis: occurrence of trehalose and molecular cloning and characterization of trehalose-6-phosphate synthase homologues."
    Vogel G., Fiehn O., Jean-Richard-dit-Bressel L., Boller T., Wiemken A., Aeschbacher R.A., Wingler A.
    J. Exp. Bot. 52:1817-1826(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Landsberg erecta.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Isolation and characterization of Arabidopsis trehalose-6-phosphate synthase."
    Kim Y.S., Lee E.J., Kim J.S., Lim D.H., Kim C.K., Chung C.H.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 31-856.
  6. "An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana."
    Leyman B., Van Dijck P., Thevelein J.M.
    Trends Plant Sci. 6:510-513(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiTPS8_ARATH
AccessioniPrimary (citable) accession number: Q0WUI9
Secondary accession number(s): O64608
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: September 5, 2006
Last modified: April 29, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.