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Protein

Subtilisin-like protease SBT6.1

Gene

SBT6.1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease that catalyzes the first step (site-1 cleavage) in the proteolytic activation of various factors, prior to site-2 cleavage. Part of a regulated intramembrane proteolysis (RIP) cascade. Cleaves BZIP17 and BZIP28 after the Arg-Arg-Ile-Leu (RRIL) motif. May cleave BZIP49 after the RRIL motif. Targets the membrane-associated BZIP17 factor, which functions as a stress sensor and transducer in a signaling pathway that resembles an ER stress response. Following salt stress, BZIP17 is cleaved by SBT6.1 (S1P) and S2P at the C-terminus and the N-terminal bZIP component is translocated to the nucleus, where it activates the expression of salt stress response genes (PubMed:17662035). Cleaves the pectinesterases PME1 after the Arg-Arg-Leu-Met (RRLM) and Arg-Arg-Leu-Leu (RRLL) motifs, and PME5 after the Arg-Arg-Leu-Leu (RRLL) and Arg-Lys-Leu-Met (RKLM) motifs. This processing and C-terminus release occurs in the Golgi apparatus and is required for cell wall targeting of pectinesterases. Thus, SBT6.1 mediates the regulated release of mature pectinesterases from the Golgi (PubMed:19144003). Cleaves the peptide growth factor RALF23 after the Arg-Arg-Ile-Leu (RRIL) motif. This processing is required for RALF23 function in the negative regulation of brassinolide (BL)-mediated signaling pathway (e.g. BL-induced hypocotyl elongation and branching limitation) (PubMed:19473327).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei212Charge relay systemPROSITE-ProRule annotation1
Active sitei243Charge relay systemPROSITE-ProRule annotation1
Active sitei409Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

  • endopeptidase activity Source: TAIR
  • serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  • hyperosmotic response Source: TAIR
  • hyperosmotic salinity response Source: TAIR
  • proteolysis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS08.063.

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin-like protease SBT6.11 Publication (EC:3.4.21.-Curated)
Alternative name(s):
Site-1 protease1 Publication
Short name:
AtS1P1 Publication
Subtilase subfamily 6 member 11 Publication
Short name:
AtSBT6.11 Publication
Gene namesi
Name:SBT6.11 Publication
Synonyms:S1PCurated
Ordered Locus Names:At5g19660Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G19660.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini182 – 1000Lumenal1 PublicationAdd BLAST819
Transmembranei1001 – 1021HelicalSequence analysisAdd BLAST21
Topological domaini1022 – 1038Cytoplasmic1 PublicationAdd BLAST17

GO - Cellular componenti

  • cell wall Source: GO_Central
  • Golgi apparatus Source: TAIR
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Short root (PubMed:20876872). Mutant plants have increased sensitivity to salt-induced osmotic stress (PubMed:17662035).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
PropeptideiPRO_000043196831 – 181Removed in mature form1 PublicationAdd BLAST151
ChainiPRO_0000431969182 – 1038Subtilisin-like protease SBT6.1Sequence analysisAdd BLAST857

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi44N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi52N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi171N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi175N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi230N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi300N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi513N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi579N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi902N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi954N-linked (GlcNAc...)PROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ0WUG6.

Expressioni

Tissue specificityi

Expressed in the vasculature of roots, cotyledons and leaves.2 Publications

Gene expression databases

GenevisibleiQ0WUG6. AT.

Interactioni

Subunit structurei

Interacts with PME1 and PME5.1 Publication

Protein-protein interaction databases

STRINGi3702.AT5G19660.1.

Structurei

3D structure databases

ProteinModelPortaliQ0WUG6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini208 – 468Peptidase S8Sequence analysisAdd BLAST261

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1025 – 1030Poly-ArgSequence analysis6

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Sequence analysis

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4266. Eukaryota.
COG1404. LUCA.
HOGENOMiHOG000030053.
KOiK08653.
OMAiVIFSDWY.
OrthoDBiEOG093601PF.
PhylomeDBiQ0WUG6.

Family and domain databases

Gene3Di3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 3 hits.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
PROSITEiPS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0WUG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLGEASSY PYRSCIIVVF LSVSLFWLRP STYHPQQQNL NPENVTRLES
60 70 80 90 100
ENETKTNYII RFKQYKPAKD HRIYLESKVR SGGWGWIERI NPATKYPTDF
110 120 130 140 150
GVLWIEESGK EAVVGEIERL EMVKDVNVEF KYQRVLLGGS FPDGKKRPGK
160 170 180 190 200
IFTSMSFEEG TESSPMADTS NTTLNWSRHL LAQKTQVTSM FGADHLWKKG
210 220 230 240 250
YTGAKVKMAI FDTGIRADHP HFRKIKERTN WTNEDTLNDN LGHGTFVAGV
260 270 280 290 300
IAGRNPECLG FASDTEIYAF RVFTDAQVSY TSWFLDAFNY AIATDMDVLN
310 320 330 340 350
LSIGGPDYLD LPFVEKVWEI TASNIIMVSA IGNDGPLYGT LNNPADQSDV
360 370 380 390 400
IGVGGIDNDD HIASFSSRGM STWELPHGYG RVKPDVVAYG RDIMGSKIST
410 420 430 440 450
GCKSLSGTSV ASPVVAGIVC LLVSVIPEAR RKDLLNPASM KQALVEGAAK
460 470 480 490 500
LSGPNMYEQG AGRVDLLESY EILKSYHPRA SIFPSILDYN DCPYSWPFCR
510 520 530 540 550
QPLYAGAMPI IFNTTILNGM GVIGYIESPP TWHPANEEGN LLSIHFKYPD
560 570 580 590 600
VIWPWTGYLA LHMQIKEEGA QFTGEIEGNV TVKVYSPPAS GESGPRRSTC
610 620 630 640 650
SLQLKLKVIP TPPRAKRILW DQFHSIKYPP GYIPRDSLDV RNDILDWHGD
660 670 680 690 700
HLHTNFHIMY NMLRDAGYYI ETLGSPLTCF DAQQYGTLLM VDLEDDYFPE
710 720 730 740 750
EIEKLRDDVI NTGLGLVVFA EWYNVDTMVK MRFFDDNTRS WWTPVTGGAN
760 770 780 790 800
IPALNNLLAS FGIAFGDKIL NGDFSIDGEQ SRYASGTNIV RFPAGGFLHT
810 820 830 840 850
FPLLDSSESG ATQNLLLTEA SKEDPAVLGL LEIGEGRVGV YGDSNCLDSS
860 870 880 890 900
HMVTNCYWLL KKMLDFSSSN IKDPVLFSKF AKRYSPVIID EKQLPSRRTD
910 920 930 940 950
VNFSTYSSVI GKELICESDS RFEVWGTKGY NLHVRGRNRR LPGYHGIDLG
960 970 980 990 1000
RGLNFTVESK RPTRWRSAKE GGELSSSRSK SLGGLFNRDE IDMPFLVPTR
1010 1020 1030
WIVLAGVVAS GVLVLLSIWR IRQKRGRRRR ASGSNRLA
Length:1,038
Mass (Da):116,151
Last modified:September 5, 2006 - v1
Checksum:i1446BDD8E36366D4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti429A → S in AAM97020 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296838 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92735.1.
AY136354 mRNA. Translation: AAM97020.1.
AK227193 mRNA. Translation: BAE99232.1.
RefSeqiNP_197467.1. NM_121971.3.
UniGeneiAt.31269.

Genome annotation databases

EnsemblPlantsiAT5G19660.1; AT5G19660.1; AT5G19660.
GeneIDi832086.
GrameneiAT5G19660.1; AT5G19660.1; AT5G19660.
KEGGiath:AT5G19660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296838 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92735.1.
AY136354 mRNA. Translation: AAM97020.1.
AK227193 mRNA. Translation: BAE99232.1.
RefSeqiNP_197467.1. NM_121971.3.
UniGeneiAt.31269.

3D structure databases

ProteinModelPortaliQ0WUG6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G19660.1.

Protein family/group databases

MEROPSiS08.063.

Proteomic databases

PaxDbiQ0WUG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G19660.1; AT5G19660.1; AT5G19660.
GeneIDi832086.
GrameneiAT5G19660.1; AT5G19660.1; AT5G19660.
KEGGiath:AT5G19660.

Organism-specific databases

TAIRiAT5G19660.

Phylogenomic databases

eggNOGiKOG4266. Eukaryota.
COG1404. LUCA.
HOGENOMiHOG000030053.
KOiK08653.
OMAiVIFSDWY.
OrthoDBiEOG093601PF.
PhylomeDBiQ0WUG6.

Miscellaneous databases

PROiQ0WUG6.

Gene expression databases

GenevisibleiQ0WUG6. AT.

Family and domain databases

Gene3Di3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 3 hits.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
PROSITEiPS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSBT61_ARATH
AccessioniPrimary (citable) accession number: Q0WUG6
Secondary accession number(s): Q8L7B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: September 5, 2006
Last modified: November 30, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.