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Protein

DnaJ protein ERDJ2A

Gene

ERDJ2A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for integral membrane and secreted preprotein translocation across the endoplasmic reticulum membrane.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-ATH-381038. XBP1(S) activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ protein ERDJ2A
Alternative name(s):
Chaperone protein dnaJ 21
Short name:
AtDjC21
Short name:
AtJ21
Endoplasmic reticulum dnaJ domain-containing protein 2A
Short name:
AtERdj2A
Translocation protein SEC63 homolog ERDJ2A
Gene namesi
Name:ERDJ2A
Synonyms:C21
Ordered Locus Names:At1g79940
ORF Names:F18B13.2, F19K16.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G79940.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88LumenalSequence analysis
Transmembranei9 – 2921Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini30 – 6536CytoplasmicSequence analysisAdd
BLAST
Transmembranei66 – 8621Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini87 – 190104LumenalSequence analysisAdd
BLAST
Transmembranei191 – 21121Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini212 – 687476CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • Golgi apparatus Source: TAIR
  • integral component of endoplasmic reticulum membrane Source: TAIR
  • mitochondrion Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Male gametophytic lethal due to defect in pollen germination and pollen tube growth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 687687DnaJ protein ERDJ2APRO_0000430364Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi90 – 901N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ0WT48.
PRIDEiQ0WT48.

PTM databases

iPTMnetiQ0WT48.
SwissPalmiQ0WT48.

Expressioni

Tissue specificityi

Expressed in leaves, flower buds and flowers.1 Publication

Inductioni

By tunicamycin.1 Publication

Gene expression databases

ExpressionAtlasiQ0WT48. baseline and differential.
GenevisibleiQ0WT48. AT.

Interactioni

Subunit structurei

Interacts with OEP61/TPR7.1 Publication

Protein-protein interaction databases

BioGridi29552. 21 interactions.
STRINGi3702.AT1G79940.1.

Structurei

3D structure databases

ProteinModelPortaliQ0WT48.
SMRiQ0WT48. Positions 99-164, 245-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini99 – 16466JPROSITE-ProRule annotationAdd
BLAST
Domaini205 – 603399SEC63Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi621 – 68767Glu-richAdd
BLAST

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation
Contains 1 SEC63 domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0721. Eukaryota.
KOG0951. Eukaryota.
COG5407. LUCA.
HOGENOMiHOG000240711.
InParanoidiQ0WT48.
KOiK09540.
OMAiWVVMIFL.
OrthoDBiEOG093605RR.
PhylomeDBiQ0WT48.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR014756. Ig_E-set.
IPR004179. Sec63-dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF02889. Sec63. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
SM00973. Sec63. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF81296. SSF81296. 2 hits.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q0WT48-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASEENSAL FPIFILTIMA IPLVPYTMVK LSGALSKKQR TIHCQCLECD
60 70 80 90 100
RSGKYKRSLF KKISNFSTWS NLTLVLLWVV MIFLIYYTKN MSREAQVFDP
110 120 130 140 150
FSILGLEPGV TDSEIKKAYR RLSIQYHPDK NPDPEANKYF VEFISKAYQA
160 170 180 190 200
LTDSVSRENF EKYGHPDGRQ GFQMGIALPQ FLLDIDGASG GILLLWIVGV
210 220 230 240 250
CILLPLVIAV IYLSRSSKYT GNYVMHQTLS AYYYLMKPSL APSKVMEVFT
260 270 280 290 300
KAAEYMEIPV RRTDDEPLQK LFMSVRSELN LDLKNMKQEQ AKFWKQHPAI
310 320 330 340 350
VKTELLIQAQ LTRESGVLSP ALQGDFRRVL ELAPRLLEEL LKMAVIPRTA
360 370 380 390 400
QGHGWLRPAV GVVELSQCIV QAVPLSARKS SGVSSEGISP FLQLPHFSDA
410 420 430 440 450
VVKKIARKKV KSFQDLQEMR LEDRSELLTQ VAGLSATDVE DIEKVLEMMP
460 470 480 490 500
SITVDITCET EGEEGIQEGD IVTLQAWVTL KRPNGLVGAL PHAPYFPFHK
510 520 530 540 550
EENYWVLLAD SVSNNVWFSQ KVSFLDEGGA ITAASKAISE SMEGSGAGVK
560 570 580 590 600
ETNDAVREAI EKVKGGSRLV MGKLQAPAEG TYNLTCFCLC DTWIGCDKKQ
610 620 630 640 650
ALKVKVLKRT RAGTRGLVSD EGAIAEEGME EEDEIEEEDY DDDYESEYSE
660 670 680
DEDEKKDMDE KRGSKKANGS VKQKKESSSE ESGSEEE
Length:687
Mass (Da):76,904
Last modified:September 5, 2006 - v1
Checksum:i7F9989FDECD529F7
GO

Sequence cautioni

The sequence AAD55462 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAG52236 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009322 Genomic DNA. Translation: AAD55462.1. Sequence problems.
AC011717 Genomic DNA. Translation: AAG52236.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36329.1.
CP002684 Genomic DNA. Translation: AEE36330.1.
CP002684 Genomic DNA. Translation: AEE36331.1.
AK227714 mRNA. Translation: BAE99700.1.
PIRiF96830.
RefSeqiNP_001031306.2. NM_001036229.2. [Q0WT48-1]
NP_001117623.1. NM_001124151.1. [Q0WT48-1]
NP_178112.2. NM_106643.4. [Q0WT48-1]
UniGeneiAt.34014.

Genome annotation databases

EnsemblPlantsiAT1G79940.1; AT1G79940.1; AT1G79940. [Q0WT48-1]
AT1G79940.2; AT1G79940.2; AT1G79940. [Q0WT48-1]
AT1G79940.3; AT1G79940.3; AT1G79940. [Q0WT48-1]
GeneIDi844334.
KEGGiath:AT1G79940.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009322 Genomic DNA. Translation: AAD55462.1. Sequence problems.
AC011717 Genomic DNA. Translation: AAG52236.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36329.1.
CP002684 Genomic DNA. Translation: AEE36330.1.
CP002684 Genomic DNA. Translation: AEE36331.1.
AK227714 mRNA. Translation: BAE99700.1.
PIRiF96830.
RefSeqiNP_001031306.2. NM_001036229.2. [Q0WT48-1]
NP_001117623.1. NM_001124151.1. [Q0WT48-1]
NP_178112.2. NM_106643.4. [Q0WT48-1]
UniGeneiAt.34014.

3D structure databases

ProteinModelPortaliQ0WT48.
SMRiQ0WT48. Positions 99-164, 245-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29552. 21 interactions.
STRINGi3702.AT1G79940.1.

PTM databases

iPTMnetiQ0WT48.
SwissPalmiQ0WT48.

Proteomic databases

PaxDbiQ0WT48.
PRIDEiQ0WT48.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G79940.1; AT1G79940.1; AT1G79940. [Q0WT48-1]
AT1G79940.2; AT1G79940.2; AT1G79940. [Q0WT48-1]
AT1G79940.3; AT1G79940.3; AT1G79940. [Q0WT48-1]
GeneIDi844334.
KEGGiath:AT1G79940.

Organism-specific databases

TAIRiAT1G79940.

Phylogenomic databases

eggNOGiKOG0721. Eukaryota.
KOG0951. Eukaryota.
COG5407. LUCA.
HOGENOMiHOG000240711.
InParanoidiQ0WT48.
KOiK09540.
OMAiWVVMIFL.
OrthoDBiEOG093605RR.
PhylomeDBiQ0WT48.

Enzyme and pathway databases

ReactomeiR-ATH-381038. XBP1(S) activates chaperone genes.

Miscellaneous databases

PROiQ0WT48.

Gene expression databases

ExpressionAtlasiQ0WT48. baseline and differential.
GenevisibleiQ0WT48. AT.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR014756. Ig_E-set.
IPR004179. Sec63-dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF02889. Sec63. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
SM00973. Sec63. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF81296. SSF81296. 2 hits.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNJ21_ARATH
AccessioniPrimary (citable) accession number: Q0WT48
Secondary accession number(s): Q9CA96, Q9SSD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 2014
Last sequence update: September 5, 2006
Last modified: September 7, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.