Reviewed, UniProtKB/Swiss-Prot Q0WSF1 (AL221_ARATH)

Names and origin

Protein names

Recommended name:
    Aldehyde dehydrogenase 22A1
    EC=1.2.1.3
Alternative name(s):
    Novel aldehyde dehydrogenase family 22 member A1

Gene names

Name:	ALDH22A1
Ordered Locus Names:	At3g66658
ORF Names:	T8E24.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	An aldehyde + NAD(+) + H(2)O = an acid + NADH.
Subcellular location	SecretedPotential.
Tissue specificity	Constituively expressed at low levels.
Induction	Not induced by abscisic acid (ABA), dehydration and salt stress.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.
Sequence caution	The sequence AAG50992.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aldehyde dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Notes: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q0WSF1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 596	566	Aldehyde dehydrogenase 22A1		PRO_0000256069
Regions
	Nucleotide binding	276 – 281	6	NAD By similarity
Sites
	Active site	298	1	Proton acceptor By similarity
	Active site	332	1	Nucleophile By similarity
	Site	199	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	33	1	V → A in BAE99947. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 25AC39BC26E4C1A8	FASTA	596	66,003
	10 20 30 40 50 60 MPFWWPLIVL AFAYAICKFL LMLIPPNVPS IDVDASDVLA HGKDTEENSF IYIPPRGRSQ 70 80 90 100 110 120 QSDKKVQCYE PATMKYLGYF PALSPTEVEE RVTLSRKAQK TWAQSSFKLR RQFLRILLKY 130 140 150 160 170 180 IIEHQELICE VSSRDTGKTM VDASLGEIMT TCEKITWLLS EGERWLKPES RSSGRAMLHK 190 200 210 220 230 240 VSRVEFHPLG VIGAIVPWNY PFHNIFNPML AAVFSGNGIV IKVSEHASWS GCFYFRIIQA 250 260 270 280 290 300 ALAAVGAPEN LVDVITGFAE TGEALVSSVD KMIFVGSTAV GKMIMRNAAE TLTPVTLELG 310 320 330 340 350 360 GKDAFIICED ADVSHVAQVA VRGTLQSSGQ NCAGAERFYV HKDIYTAFIG QVTKIVKSVS 370 380 390 400 410 420 AGPPLTGRYD MGAICLQEHS EHLQSLVNDA LDKGAEIAVR GSFGHLGEDA VDQYFPPTVL 430 440 450 460 470 480 INVNHNMKIM KEEAFGPIMP IMQFSTDEEV IKLANDSRYA LGCAVFSGSK HRAKQIASQI 490 500 510 520 530 540 QCGVAAINDF ASNYMCQSLP FGGVKDSGFG RFAGIEGLRA CCLVKSVVED RFWPLIKTKI 550 560 570 580 590 PKPIQYPVAE NAFEFQEALV ETLYGLNIWD RLRSLIDVLK FLTDQSSNVS RTRKSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Detailed expression analysis of selected genes of the aldehyde dehydrogenase(ALDH) gene superfamily in Arabidopsis thaliana." Kirch H.-H., Schlingensiepen S., Kotchoni S., Sunkar R., Bartels D. Plant Mol. Biol. 57:315-322(2005) [PubMed: 15830124] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The ALDH gene superfamily of Arabidopsis." Kirch H.-H., Bartels D., Wei Y., Schnable P.S., Wood A.J. Trends Plant Sci. 9:371-377(2004) [PubMed: 15358267] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ584646 mRNA. Translation: CAE48165.1. AC036106 Genomic DNA. Translation: AAG50992.1. Sequence problems. AK227981 mRNA. Translation: BAE99947.1.
RefSeq	NP_974242.1.
UniGene	At.43176
3D structure databases
HSSP	HSSP built from PDB template 1AD3 based on UniProtKB P11883.
ModBase	Search...
Genome annotation databases
GeneID	819849.
GenomeReviews	Gene locus AT3G66658 in contig BA000014_GR.
NMPDR	fig|3702.1.peg.12680.
Organism-specific databases
TAIR	At3g66658.
Family and domain databases
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AL221_ARATH

Accession

Primary (citable) accession number: Q0WSF1
Secondary accession number(s): Q70E95, Q9C837

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	November 4, 2008
This is version 21 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)