Reviewed, UniProtKB/Swiss-Prot Q0WRC9 (SKI17_ARATH)

Names and origin

Protein names

Recommended name:
    F-box protein SKIP17
Alternative name(s):
    SKP1-interacting partner 17

Gene names

Name:	SKIP17
Ordered Locus Names:	At4g02740
ORF Names:	T5J8.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (ASK-cullin-F-box) protein ligase complex By similarity. Interacts with SPK1B/ASK2.
Subcellular location	Nucleus By similarity.
Domain	The F-box is necessary for the interaction with ASK proteins By similarity.
Sequence similarities	Contains 1 F-box domain.
Sequence caution	The sequence AAD15349.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB77759.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	protein binding Ref.3 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SKP1B	Q9FHW7	2	EBI-604757,EBI-604076

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	F-box protein SKIP17		PRO_0000283487
Regions
	Domain	92 – 138	47	F-box
Experimental info
	Sequence conflict	280	1	F → L in BAD42970. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q0WRC9-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: D20CD4CEB7E6346A	FASTA	479	53,680
	10 20 30 40 50 60 MDPPTQSHNS VRTVPGSNHS HTDLIISSFL SFPDSSPISI SNSFDRVLDR ALASASADES 70 80 90 100 110 120 VQDRLVDRTL ELASLLLDST RRCFRKRASV HNSNSWSLPP ELTIKVFSML DTKSMMQAAV 130 140 150 160 170 180 CCTMFNKCAM DRLCYSHIDL TTSARYADKG VVSTMINRAG KELRSLKLGR VVRTAGSDSA 190 200 210 220 230 240 APLLSGSCLS PLAYNHGFLG SRLRSLRLYN LRPIKYRSLC DALSVCPNIT DLRIVGLYNL 250 260 270 280 290 300 TEELFNSLTK KCRLIEHLFL ETYGYPRTLE SKAGSSLVEF VTNCPNLTSL TLIRFGLTDD 310 320 330 340 350 360 WARNLAESCR KLKYLNLSRS PTIKGRFLRE LGLSCKENLL KTLILRSCPK LQEKEVLEFC 370 380 390 400 410 420 NSLLTGNFKS IRQIDVSSNS GLASSDRGKR CNKPNFPLER LKEERSDVTF VADFPSTSSG 430 440 450 460 470 KRYGVCDEEE LRLIEMMEAE DDEVDEEDDS DDDTDDVSDE DESENDDDMG MGFDVDYLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed: 12795696] [Abstract] Cited for: INTERACTION WITH SPK1B/ASK2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC004044 Genomic DNA. Translation: AAD15349.1. Sequence problems. AL161495 Genomic DNA. Translation: CAB77759.1. Sequence problems. AK175207 mRNA. Translation: BAD42970.1. AK228382 mRNA. Translation: BAF00320.1.
IPI	IPI00520192.
PIR	H85034.
RefSeq	NP_192183.2.
UniGene	At.43357
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q0WRC9. 7 interactions.
Proteomic databases
PRIDE	Q0WRC9.
Genome annotation databases
GeneID	828188.
GenomeReviews	Gene locus AT4G02740 in contig CT486007_GR.
KEGG	ath:AT4G02740.
Organism-specific databases
TAIR	At4g02740.
Family and domain databases
InterPro	IPR001810. F-box. [Graphical view]
Pfam	PF00646. F-box. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SKI17_ARATH

Accession

Primary (citable) accession number: Q0WRC9
Secondary accession number(s): Q683B0, Q9SY01

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	September 5, 2006
Last modified:	May 26, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)