Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pentatricopeptide repeat-containing protein At1g59720, chloroplastic/mitochondrial

Gene

PCMP-H51

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in multiple sites RNA editing events in chloroplasts. Involved in the editing of the site 2 of ndhB (ndhB-2) and site 3 of ndhD (ndhD-3) transcripts, which are two plastid-encoded subunits of the chloroplast NAD(P)H dehydrogenase (NDH) complex. Required for the activity of the NDH complex of the photosynthetic electron transport chain.1 Publication

GO - Molecular functioni

  • endonuclease activity Source: TAIR
  • zinc ion binding Source: InterPro

GO - Biological processi

  • mRNA modification Source: TAIR
  • mRNA processing Source: UniProtKB-KW
  • nucleic acid phosphodiester bond hydrolysis Source: GOC
Complete GO annotation...

Keywords - Biological processi

mRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Pentatricopeptide repeat-containing protein At1g59720, chloroplastic/mitochondrialCurated
Alternative name(s):
Protein CHLORORESPIRATORY REDUCTION 281 Publication
Gene namesi
Name:PCMP-H51
Synonyms:CRR281 Publication
Ordered Locus Names:At1g59720
ORF Names:F23H11.3, T30E16.32
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G59720.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Impaired chloroplastic NAD(P)H dehydrogenase (NDH) activity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4040Chloroplast and mitochondrionSequence analysisAdd
BLAST
Chaini41 – 638598Pentatricopeptide repeat-containing protein At1g59720, chloroplastic/mitochondrialSequence analysisPRO_0000342826Add
BLAST

Proteomic databases

PaxDbiQ0WQW5.
PRIDEiQ0WQW5.

Expressioni

Gene expression databases

GenevisibleiQ0WQW5. AT.

Interactioni

Protein-protein interaction databases

BioGridi27488. 1 interaction.
STRINGi3702.AT1G59720.1.

Structurei

3D structure databases

ProteinModelPortaliQ0WQW5.
SMRiQ0WQW5. Positions 38-499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati82 – 11231PPR 1Add
BLAST
Repeati113 – 14836PPR 2Add
BLAST
Repeati150 – 18435PPR 3Add
BLAST
Repeati185 – 21531PPR 4Add
BLAST
Repeati216 – 24631PPR 5Add
BLAST
Repeati250 – 28031PPR 6Add
BLAST
Repeati288 – 31831PPR 7Add
BLAST
Repeati319 – 35335PPR 8Add
BLAST
Repeati356 – 39035PPR 9Add
BLAST
Repeati392 – 42231PPR 10Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni427 – 51084Type E motifAdd
BLAST
Regioni511 – 54131Type E(+) motifAdd
BLAST
Regioni542 – 63897Type DYW motifAdd
BLAST

Sequence similaritiesi

Belongs to the PPR family. PCMP-H subfamily.Curated
Contains 10 PPR (pentatricopeptide) repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG4197. Eukaryota.
ENOG410Z7Z7. LUCA.
HOGENOMiHOG000237569.
InParanoidiQ0WQW5.
OMAiLYSRILH.
PhylomeDBiQ0WQW5.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR032867. DYW_dom.
IPR002885. Pentatricopeptide_repeat.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF14432. DYW_deaminase. 1 hit.
PF01535. PPR. 2 hits.
PF13041. PPR_2. 2 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
TIGRFAMsiTIGR00756. PPR. 4 hits.
PROSITEiPS51375. PPR. 10 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0WQW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVRSIIVSP PTTITYYHPM SIGLLVHPLS PHIPPASSPS ASTAGNHHQR
60 70 80 90 100
IFSLAETCSD MSQLKQLHAF TLRTTYPEEP ATLFLYGKIL QLSSSFSDVN
110 120 130 140 150
YAFRVFDSIE NHSSFMWNTL IRACAHDVSR KEEAFMLYRK MLERGESSPD
160 170 180 190 200
KHTFPFVLKA CAYIFGFSEG KQVHCQIVKH GFGGDVYVNN GLIHLYGSCG
210 220 230 240 250
CLDLARKVFD EMPERSLVSW NSMIDALVRF GEYDSALQLF REMQRSFEPD
260 270 280 290 300
GYTMQSVLSA CAGLGSLSLG TWAHAFLLRK CDVDVAMDVL VKNSLIEMYC
310 320 330 340 350
KCGSLRMAEQ VFQGMQKRDL ASWNAMILGF ATHGRAEEAM NFFDRMVDKR
360 370 380 390 400
ENVRPNSVTF VGLLIACNHR GFVNKGRQYF DMMVRDYCIE PALEHYGCIV
410 420 430 440 450
DLIARAGYIT EAIDMVMSMP MKPDAVIWRS LLDACCKKGA SVELSEEIAR
460 470 480 490 500
NIIGTKEDNE SSNGNCSGAY VLLSRVYASA SRWNDVGIVR KLMSEHGIRK
510 520 530 540 550
EPGCSSIEIN GISHEFFAGD TSHPQTKQIY QQLKVIDDRL RSIGYLPDRS
560 570 580 590 600
QAPLVDATND GSKEYSLRLH SERLAIAFGL INLPPQTPIR IFKNLRVCND
610 620 630
CHEVTKLISK VFNTEIIVRD RVRFHHFKDG SCSCLDYW
Length:638
Mass (Da):71,960
Last modified:July 1, 2008 - v2
Checksum:iB868192817B41756
GO

Sequence cautioni

The sequence AAD39314.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAF79766.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007258 Genomic DNA. Translation: AAD39314.1. Sequence problems.
AC009317 Genomic DNA. Translation: AAF79766.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33607.1.
AK228565 mRNA. Translation: BAF00484.1.
PIRiH96620.
RefSeqiNP_176180.1. NM_104664.2.
UniGeneiAt.11368.
At.66916.

Genome annotation databases

EnsemblPlantsiAT1G59720.1; AT1G59720.1; AT1G59720.
GeneIDi842263.
GrameneiAT1G59720.1; AT1G59720.1; AT1G59720.
KEGGiath:AT1G59720.

Cross-referencesi

Web resourcesi

Arabidopsis PPR Protein Database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007258 Genomic DNA. Translation: AAD39314.1. Sequence problems.
AC009317 Genomic DNA. Translation: AAF79766.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33607.1.
AK228565 mRNA. Translation: BAF00484.1.
PIRiH96620.
RefSeqiNP_176180.1. NM_104664.2.
UniGeneiAt.11368.
At.66916.

3D structure databases

ProteinModelPortaliQ0WQW5.
SMRiQ0WQW5. Positions 38-499.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27488. 1 interaction.
STRINGi3702.AT1G59720.1.

Proteomic databases

PaxDbiQ0WQW5.
PRIDEiQ0WQW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G59720.1; AT1G59720.1; AT1G59720.
GeneIDi842263.
GrameneiAT1G59720.1; AT1G59720.1; AT1G59720.
KEGGiath:AT1G59720.

Organism-specific databases

TAIRiAT1G59720.

Phylogenomic databases

eggNOGiKOG4197. Eukaryota.
ENOG410Z7Z7. LUCA.
HOGENOMiHOG000237569.
InParanoidiQ0WQW5.
OMAiLYSRILH.
PhylomeDBiQ0WQW5.

Miscellaneous databases

PROiQ0WQW5.

Gene expression databases

GenevisibleiQ0WQW5. AT.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR032867. DYW_dom.
IPR002885. Pentatricopeptide_repeat.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF14432. DYW_deaminase. 1 hit.
PF01535. PPR. 2 hits.
PF13041. PPR_2. 2 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
TIGRFAMsiTIGR00756. PPR. 4 hits.
PROSITEiPS51375. PPR. 10 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-638.
    Strain: cv. Columbia.
  4. "In Arabidopsis thaliana, 1% of the genome codes for a novel protein family unique to plants."
    Aubourg S., Boudet N., Kreis M., Lecharny A.
    Plant Mol. Biol. 42:603-613(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  5. "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins reveals their essential role in organelle biogenesis."
    Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C., Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M., Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B., Taconnat L., Small I.
    Plant Cell 16:2089-2103(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, SUBCELLULAR LOCATION.
  6. "Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts."
    Okuda K., Chateigner-Boutin A.L., Nakamura T., Delannoy E., Sugita M., Myouga F., Motohashi R., Shinozaki K., Small I., Shikanai T.
    Plant Cell 21:146-156(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPPR85_ARATH
AccessioniPrimary (citable) accession number: Q0WQW5
Secondary accession number(s): Q9LQ42, Q9XIF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: May 11, 2016
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The DYW motif is dispensable for editing activity in vivo.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.