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Protein

Dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrial

Gene

LTA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).1 Publication

Catalytic activityi

Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine.

Cofactori

(R)-lipoateNote: Binds 2 lipoyl cofactors covalently.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei610Sequence analysis1
Active sitei614Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Glycolysis

Enzyme and pathway databases

BioCyciARA:AT3G52200-MONOMER.
ReactomeiR-ATH-70268. Pyruvate metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrial (EC:2.3.1.12)
Alternative name(s):
Dihydrolipoamide S-acetyltransferase component 1 of pyruvate dehydrogenase complex
Pyruvate dehydrogenase complex component E2 1
Short name:
PDC-E2 1
Short name:
PDCE2 1
Gene namesi
Name:LTA3
Ordered Locus Names:At3g52200
ORF Names:F4F15.310
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G52200.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • pyruvate dehydrogenase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 84MitochondrionSequence analysisAdd BLAST84
ChainiPRO_000026002585 – 637Dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrialAdd BLAST553

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei126N6-lipoyllysinePROSITE-ProRule annotationBy similarity1
Modified residuei253N6-lipoyllysinePROSITE-ProRule annotationBy similarity1

Proteomic databases

PaxDbiQ0WQF7.
PRIDEiQ0WQF7.

PTM databases

SwissPalmiQ0WQF7.

Expressioni

Gene expression databases

ExpressionAtlasiQ0WQF7. baseline and differential.
GenevisibleiQ0WQF7. AT.

Interactioni

Protein-protein interaction databases

BioGridi9703. 5 interactors.
MINTiMINT-8060819.
STRINGi3702.AT3G52200.2.

Structurei

3D structure databases

ProteinModelPortaliQ0WQF7.
SMRiQ0WQF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini85 – 161Lipoyl-binding 1PROSITE-ProRule annotationAdd BLAST77
Domaini212 – 288Lipoyl-binding 2PROSITE-ProRule annotationAdd BLAST77

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni332 – 363E3-binding siteBy similarityAdd BLAST32

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.Curated
Contains 2 lipoyl-binding domains.PROSITE-ProRule annotationCurated

Keywords - Domaini

Lipoyl, Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0557. Eukaryota.
COG0508. LUCA.
HOGENOMiHOG000281566.
InParanoidiQ0WQF7.
KOiK00627.
PhylomeDBiQ0WQF7.

Family and domain databases

Gene3Di3.30.559.10. 1 hit.
4.10.320.10. 1 hit.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR001078. 2-oxoacid_DH_actylTfrase.
IPR000089. Biotin_lipoyl.
IPR023213. CAT-like_dom.
IPR004167. E3-bd.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamiPF00198. 2-oxoacid_dh. 1 hit.
PF00364. Biotin_lipoyl. 2 hits.
PF02817. E3_binding. 1 hit.
[Graphical view]
SUPFAMiSSF47005. SSF47005. 1 hit.
SSF51230. SSF51230. 2 hits.
PROSITEiPS50968. BIOTINYL_LIPOYL. 2 hits.
PS00189. LIPOYL. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q0WQF7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLPLFRRAA IARTSSLLRA RLFAPASEFH SRFSNGLYHL DDKISSSNGV
60 70 80 90 100
RSASIDLITR MDDSSPKPIL RFGVQNFSST GPISQTVLAM PALSPTMSHG
110 120 130 140 150
NVVKWMKKEG DKVEVGDVLC EIETDKATVE FESQEEGFLA KILVTEGSKD
160 170 180 190 200
IPVNEPIAIM VEEEDDIKNV PATIEGGRDG KEETSAHQVM KPDESTQQKS
210 220 230 240 250
SIQPDASDLP PHVVLEMPAL SPTMNQGNIA KWWKKEGDKI EVGDVIGEIE
260 270 280 290 300
TDKATLEFES LEEGYLAKIL IPEGSKDVAV GKPIALIVED AESIEAIKSS
310 320 330 340 350
SAGSSEVDTV KEVPDSVVDK PTERKAGFTK ISPAAKLLIL EHGLEASSIE
360 370 380 390 400
ASGPYGTLLK SDVVAAIASG KASKSSASTK KKQPSKETPS KSSSTSKPSV
410 420 430 440 450
TQSDNNYEDF PNSQIRKIIA KRLLESKQKI PHLYLQSDVV LDPLLAFRKE
460 470 480 490 500
LQENHGVKVS VNDIVIKAVA VALRNVRQAN AFWDAEKGDI VMCDSVDISI
510 520 530 540 550
AVATEKGLMT PIIKNADQKS ISAISLEVKE LAQKARSGKL APHEFQGGTF
560 570 580 590 600
SISNLGMYPV DNFCAIINPP QAGILAVGRG NKVVEPVIGL DGIEKPSVVT
610 620 630
KMNVTLSADH RIFDGQVGAS FMSELRSNFE DVRRLLL
Length:637
Mass (Da):68,862
Last modified:November 14, 2006 - v2
Checksum:iCBD20C3B1BF2B4E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6F → L in BAF00642 (Ref. 4) Curated1
Sequence conflicti378S → F in AAD55140 (Ref. 1) Curated1
Sequence conflicti583V → E in AAD55140 (Ref. 1) Curated1
Sequence conflicti583V → E in CAA86300 (PubMed:7890655).Curated1
Sequence conflicti597S → C in AAD55140 (Ref. 1) Curated1
Sequence conflicti597S → C in CAA86300 (PubMed:7890655).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF066080 mRNA. Translation: AAD55140.1.
AL049711 Genomic DNA. Translation: CAB41340.1.
CP002686 Genomic DNA. Translation: AEE78912.1.
AK228742 mRNA. Translation: BAF00642.1.
Z46230 mRNA. Translation: CAA86300.1.
PIRiA55939.
T49099.
RefSeqiNP_190788.1. NM_115080.3. [Q0WQF7-1]
UniGeneiAt.3218.

Genome annotation databases

EnsemblPlantsiAT3G52200.1; AT3G52200.1; AT3G52200. [Q0WQF7-1]
GeneIDi824385.
GrameneiAT3G52200.1; AT3G52200.1; AT3G52200.
KEGGiath:AT3G52200.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF066080 mRNA. Translation: AAD55140.1.
AL049711 Genomic DNA. Translation: CAB41340.1.
CP002686 Genomic DNA. Translation: AEE78912.1.
AK228742 mRNA. Translation: BAF00642.1.
Z46230 mRNA. Translation: CAA86300.1.
PIRiA55939.
T49099.
RefSeqiNP_190788.1. NM_115080.3. [Q0WQF7-1]
UniGeneiAt.3218.

3D structure databases

ProteinModelPortaliQ0WQF7.
SMRiQ0WQF7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9703. 5 interactors.
MINTiMINT-8060819.
STRINGi3702.AT3G52200.2.

PTM databases

SwissPalmiQ0WQF7.

Proteomic databases

PaxDbiQ0WQF7.
PRIDEiQ0WQF7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G52200.1; AT3G52200.1; AT3G52200. [Q0WQF7-1]
GeneIDi824385.
GrameneiAT3G52200.1; AT3G52200.1; AT3G52200.
KEGGiath:AT3G52200.

Organism-specific databases

TAIRiAT3G52200.

Phylogenomic databases

eggNOGiKOG0557. Eukaryota.
COG0508. LUCA.
HOGENOMiHOG000281566.
InParanoidiQ0WQF7.
KOiK00627.
PhylomeDBiQ0WQF7.

Enzyme and pathway databases

BioCyciARA:AT3G52200-MONOMER.
ReactomeiR-ATH-70268. Pyruvate metabolism.

Miscellaneous databases

PROiQ0WQF7.

Gene expression databases

ExpressionAtlasiQ0WQF7. baseline and differential.
GenevisibleiQ0WQF7. AT.

Family and domain databases

Gene3Di3.30.559.10. 1 hit.
4.10.320.10. 1 hit.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR001078. 2-oxoacid_DH_actylTfrase.
IPR000089. Biotin_lipoyl.
IPR023213. CAT-like_dom.
IPR004167. E3-bd.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamiPF00198. 2-oxoacid_dh. 1 hit.
PF00364. Biotin_lipoyl. 2 hits.
PF02817. E3_binding. 1 hit.
[Graphical view]
SUPFAMiSSF47005. SSF47005. 1 hit.
SSF51230. SSF51230. 2 hits.
PROSITEiPS50968. BIOTINYL_LIPOYL. 2 hits.
PS00189. LIPOYL. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiODP21_ARATH
AccessioniPrimary (citable) accession number: Q0WQF7
Secondary accession number(s): Q39082, Q9SQI7, Q9SUY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2006
Last sequence update: November 14, 2006
Last modified: November 30, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.