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Protein

Dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrial

Gene

LTA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).1 Publication

Catalytic activityi

Acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine.

Cofactori

(R)-lipoateNote: Binds 2 lipoyl cofactors covalently.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei610Sequence analysis1
Active sitei614Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processGlycolysis

Enzyme and pathway databases

BioCyciARA:AT3G52200-MONOMER
ReactomeiR-ATH-204174 Regulation of pyruvate dehydrogenase (PDH) complex
R-ATH-5362517 Signaling by Retinoic Acid
R-ATH-70268 Pyruvate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrial (EC:2.3.1.12)
Alternative name(s):
Dihydrolipoamide S-acetyltransferase component 1 of pyruvate dehydrogenase complex
Pyruvate dehydrogenase complex component E2 1
Short name:
PDC-E2 1
Short name:
PDCE2 1
Gene namesi
Name:LTA3
Ordered Locus Names:At3g52200
ORF Names:F4F15.310
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G52200

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 84MitochondrionSequence analysisAdd BLAST84
ChainiPRO_000026002585 – 637Dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex, mitochondrialAdd BLAST553

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei126N6-lipoyllysinePROSITE-ProRule annotationBy similarity1
Modified residuei253N6-lipoyllysinePROSITE-ProRule annotationBy similarity1

Proteomic databases

PaxDbiQ0WQF7
PRIDEiQ0WQF7

PTM databases

SwissPalmiQ0WQF7

Expressioni

Gene expression databases

ExpressionAtlasiQ0WQF7 baseline and differential
GenevisibleiQ0WQF7 AT

Interactioni

Protein-protein interaction databases

BioGridi9703, 5 interactors
STRINGi3702.AT3G52200.2

Structurei

3D structure databases

ProteinModelPortaliQ0WQF7
SMRiQ0WQF7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini85 – 161Lipoyl-binding 1PROSITE-ProRule annotationAdd BLAST77
Domaini212 – 288Lipoyl-binding 2PROSITE-ProRule annotationAdd BLAST77
Domaini330 – 367Peripheral subunit-binding (PSBD)PROSITE-ProRule annotationAdd BLAST38

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.Curated

Keywords - Domaini

Lipoyl, Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0557 Eukaryota
COG0508 LUCA
HOGENOMiHOG000281566
InParanoidiQ0WQF7
KOiK00627
PhylomeDBiQ0WQF7

Family and domain databases

Gene3Di3.30.559.10, 1 hit
4.10.320.10, 1 hit
InterProiView protein in InterPro
IPR003016 2-oxoA_DH_lipoyl-BS
IPR001078 2-oxoacid_DH_actylTfrase
IPR000089 Biotin_lipoyl
IPR023213 CAT-like_dom_sf
IPR036625 E3-bd_dom_sf
IPR004167 PSBD
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF00198 2-oxoacid_dh, 1 hit
PF00364 Biotin_lipoyl, 2 hits
PF02817 E3_binding, 1 hit
SUPFAMiSSF47005 SSF47005, 1 hit
SSF51230 SSF51230, 2 hits
PROSITEiView protein in PROSITE
PS50968 BIOTINYL_LIPOYL, 2 hits
PS00189 LIPOYL, 2 hits
PS51826 PSBD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q0WQF7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLPLFRRAA IARTSSLLRA RLFAPASEFH SRFSNGLYHL DDKISSSNGV
60 70 80 90 100
RSASIDLITR MDDSSPKPIL RFGVQNFSST GPISQTVLAM PALSPTMSHG
110 120 130 140 150
NVVKWMKKEG DKVEVGDVLC EIETDKATVE FESQEEGFLA KILVTEGSKD
160 170 180 190 200
IPVNEPIAIM VEEEDDIKNV PATIEGGRDG KEETSAHQVM KPDESTQQKS
210 220 230 240 250
SIQPDASDLP PHVVLEMPAL SPTMNQGNIA KWWKKEGDKI EVGDVIGEIE
260 270 280 290 300
TDKATLEFES LEEGYLAKIL IPEGSKDVAV GKPIALIVED AESIEAIKSS
310 320 330 340 350
SAGSSEVDTV KEVPDSVVDK PTERKAGFTK ISPAAKLLIL EHGLEASSIE
360 370 380 390 400
ASGPYGTLLK SDVVAAIASG KASKSSASTK KKQPSKETPS KSSSTSKPSV
410 420 430 440 450
TQSDNNYEDF PNSQIRKIIA KRLLESKQKI PHLYLQSDVV LDPLLAFRKE
460 470 480 490 500
LQENHGVKVS VNDIVIKAVA VALRNVRQAN AFWDAEKGDI VMCDSVDISI
510 520 530 540 550
AVATEKGLMT PIIKNADQKS ISAISLEVKE LAQKARSGKL APHEFQGGTF
560 570 580 590 600
SISNLGMYPV DNFCAIINPP QAGILAVGRG NKVVEPVIGL DGIEKPSVVT
610 620 630
KMNVTLSADH RIFDGQVGAS FMSELRSNFE DVRRLLL
Length:637
Mass (Da):68,862
Last modified:November 14, 2006 - v2
Checksum:iCBD20C3B1BF2B4E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6F → L in BAF00642 (Ref. 4) Curated1
Sequence conflicti378S → F in AAD55140 (Ref. 1) Curated1
Sequence conflicti583V → E in AAD55140 (Ref. 1) Curated1
Sequence conflicti583V → E in CAA86300 (PubMed:7890655).Curated1
Sequence conflicti597S → C in AAD55140 (Ref. 1) Curated1
Sequence conflicti597S → C in CAA86300 (PubMed:7890655).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF066080 mRNA Translation: AAD55140.1
AL049711 Genomic DNA Translation: CAB41340.1
CP002686 Genomic DNA Translation: AEE78912.1
AK228742 mRNA Translation: BAF00642.1
Z46230 mRNA Translation: CAA86300.1
PIRiA55939
T49099
RefSeqiNP_190788.1, NM_115080.3 [Q0WQF7-1]
UniGeneiAt.3218

Genome annotation databases

EnsemblPlantsiAT3G52200.1; AT3G52200.1; AT3G52200 [Q0WQF7-1]
GeneIDi824385
GrameneiAT3G52200.1; AT3G52200.1; AT3G52200 [Q0WQF7-1]
KEGGiath:AT3G52200

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiODP21_ARATH
AccessioniPrimary (citable) accession number: Q0WQF7
Secondary accession number(s): Q39082, Q9SQI7, Q9SUY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2006
Last sequence update: November 14, 2006
Last modified: April 25, 2018
This is version 101 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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