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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 3, chloroplastic

Gene

MS3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.1 Publication

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Kineticsi

  1. KM=17 µM for 5-methyltetrahydrofolate1 Publication
  1. Vmax=0.6 nmol/min/mg enzyme toward 5-methyltetrahydrofolate1 Publication

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 3, chloroplastic (MS3), 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 (MS2), 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 (MS1)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei538L-homocysteineBy similarity1
Binding sitei6155-methyltetrahydrofolateBy similarity1
Binding sitei653L-homocysteineBy similarity1
Metal bindingi695Zinc 1By similarity1
Metal bindingi697Zinc 1By similarity1
Metal bindingi706Zinc 2By similarity1
Metal bindingi710Zinc 2By similarity1
Metal bindingi781Zinc 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT5G20980-MONOMER
SABIO-RKiQ0WNZ5
UniPathwayiUPA00051; UER00082

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 3, chloroplastic (EC:2.1.1.14)
Alternative name(s):
Cobalamin-independent methionine synthase 3
Short name:
AtMS3
Gene namesi
Name:MS3
Ordered Locus Names:At5g20980
ORF Names:F22D1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G20980
TAIRilocus:2147147 AT5G20980

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33ChloroplastSequence analysisAdd BLAST33
ChainiPRO_000042435734 – 8125-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 3, chloroplasticAdd BLAST779

Proteomic databases

PaxDbiQ0WNZ5
PRIDEiQ0WNZ5

PTM databases

iPTMnetiQ0WNZ5

Expressioni

Tissue specificityi

Expressed in seeds.1 Publication

Gene expression databases

ExpressionAtlasiQ0WNZ5 baseline and differential
GenevisibleiQ0WNZ5 AT

Interactioni

Protein-protein interaction databases

IntActiQ0WNZ5 1 interactor.
MINTiQ0WNZ5
STRINGi3702.AT5G20980.1

Structurei

3D structure databases

ProteinModelPortaliQ0WNZ5
SMRiQ0WNZ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni485 – 487L-homocysteine bindingBy similarity3
Regioni569 – 5705-methyltetrahydrofolate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2263 Eukaryota
COG0620 LUCA
HOGENOMiHOG000246221
InParanoidiQ0WNZ5
KOiK00549
OMAiHMAHAGI
OrthoDBiEOG093602ZG
PhylomeDBiQ0WNZ5

Family and domain databases

Gene3Di3.20.20.2104 hits
HAMAPiMF_00172 Meth_synth, 1 hit
InterProiView protein in InterPro
IPR013215 Cbl-indep_Met_Synth_N
IPR006276 Cobalamin-indep_Met_synthase
IPR002629 Met_Synth_C/arc
IPR038071 UROD/MetE-like_sf
PfamiView protein in Pfam
PF08267 Meth_synt_1, 1 hit
PF01717 Meth_synt_2, 1 hit
PIRSFiPIRSF000382 MeTrfase_B12_ind, 1 hit
SUPFAMiSSF51726 SSF51726, 2 hits
TIGRFAMsiTIGR01371 met_syn_B12ind, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0WNZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQLALQRLQ PLASLPRRPP SLPPPSSATP SLPCATASRR PRFYVARAMS
60 70 80 90 100
SHIVGYPRIG PKRELKFALE SFWDGKTNVD DLQNVAANLR KSIWKHMAHA
110 120 130 140 150
GIKYIPSNTF SYYDQMLDTT AMLGAVPSRY GWESGEIGFD VYFSMARGNA
160 170 180 190 200
SAHAMEMTKW FDTNYHYIVP ELGPDVNFSY ASHKAVVEFK EAKALGIDTV
210 220 230 240 250
PVLIGPMTYL LLSKPAKGVE KSFCLLSLID KILPVYKEVL ADLKSAGARW
260 270 280 290 300
IQFDEPILVM DLDTSQLQAF SDAYSHMESS LAGLNVLIAT YFADVPAEAY
310 320 330 340 350
KTLMSLKCVT GFGFDLVRGL ETLDLIKMNF PRGKLLFAGV VDGRNIWAND
360 370 380 390 400
LSASLKTLQT LEDIVGKEKV VVSTSCSLLH TAVDLVNEMK LDKELKSWLA
410 420 430 440 450
FAAQKVVEVN ALAKSFSGAK DEALFSSNSM RQASRRSSPR VTNAAVQQDV
460 470 480 490 500
DAVKKSDHHR STEVSVRLQA QQKKLNLPAL PTTTIGSFPQ TTDLRRIRRE
510 520 530 540 550
FKAKKISEVD YVQTIKEEYE KVIKLQEELG IDVLVHGEAE RNDMVEFFGE
560 570 580 590 600
QLSGFAFTSN GWVQSYGSRC VKPPIIYGDI TRPKAMTVFW SSMAQKMTQR
610 620 630 640 650
PMKGMLTGPV TILNWSFVRN DQPRHETCFQ IALAIKDEVE DLEKAGVTVI
660 670 680 690 700
QIDEAALREG LPLRKSEQKF YLDWAVHAFR ITNSGVQDST QIHTHMCYSN
710 720 730 740 750
FNDIIHSIID MDADVITIEN SRSDEKLLSV FHEGVKYGAG IGPGVYDIHS
760 770 780 790 800
PRIPSTEEIA ERINKMLAVL DSKVLWVNPD CGLKTRNYSE VKSALSNMVA
810
AAKLIRSQLN KS
Length:812
Mass (Da):90,594
Last modified:September 5, 2006 - v1
Checksum:i976DC8AC06A1DF27
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11P → L in CAE55865 (PubMed:15024005).Curated1
Sequence conflicti77T → S in CAE55865 (PubMed:15024005).Curated1
Sequence conflicti634A → G in CAE55865 (PubMed:15024005).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ608675 mRNA Translation: CAE55865.1
AF296834 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED92915.1
CP002688 Genomic DNA Translation: AED92916.1
AK229291 mRNA Translation: BAF01154.1
RefSeqiNP_001078611.1, NM_001085142.1
NP_197598.2, NM_122107.4
UniGeneiAt.31122

Genome annotation databases

EnsemblPlantsiAT5G20980.1; AT5G20980.1; AT5G20980
AT5G20980.2; AT5G20980.2; AT5G20980
GeneIDi832223
GrameneiAT5G20980.1; AT5G20980.1; AT5G20980
AT5G20980.2; AT5G20980.2; AT5G20980
KEGGiath:AT5G20980

Similar proteinsi

Entry informationi

Entry nameiMETE3_ARATH
AccessioniPrimary (citable) accession number: Q0WNZ5
Secondary accession number(s): Q6KCR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: September 5, 2006
Last modified: April 25, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome