Q0WNJ6 (CLAH1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Clathrin heavy chain 1

Gene names

Name:	CHC1
Ordered Locus Names:	At3g11130
ORF Names:	F11B9.30, F9F8.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1705 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Clathrin is the major protein of the polyhedral coat of coated pits and vesicles By similarity. Mediates endocytosis and is required for a correct polar distribution of PIN auxin transporters. Ref.4
Subunit structure	Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat By similarity.
Subcellular location	Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Membrane › coated pit; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Cytoplasmic face of coated pits and vesicles By similarity.
Domain	The C-terminal third of the heavy chains forms the hub of the triskelion. This region contains the trimerization domain and the light-chain binding domain involved in the assembly of the clathrin lattice. The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It consitutes a major protein-protein interaction node By similarity.
Sequence similarities	Belongs to the clathrin heavy chain family.
Sequence caution	The sequence AAF01510.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAG50967.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Coated pit Cytoplasmic vesicle Membrane
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from mutant phenotype Ref.4. Source: TAIR intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 22430844. Source: TAIR clathrin coat of coated pit Inferred from electronic annotation. Source: InterPro clathrin coat of trans-Golgi network vesicle Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from direct assay PubMed 17317660PubMed 17644812PubMed 22923678. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR vacuolar membrane Inferred from direct assay PubMed 17151019. Source: TAIR
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EPSIN1	Q8VY07	3	EBI-1162845,EBI-1162785

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1705	1705	Clathrin heavy chain 1		PRO_0000413949
Regions
	Region	1 – 492	492	Globular terminal domain By similarity
	Region	25 – 67	43	WD40-like repeat 1
	Region	68 – 113	46	WD40-like repeat 2
	Region	114 – 155	42	WD40-like repeat 3
	Region	156 – 205	50	WD40-like repeat 4
	Region	206 – 270	65	WD40-like repeat 5
	Region	271 – 314	44	WD40-like repeat 6
	Region	315 – 343	29	WD40-like repeat 7
	Region	462 – 478	17	Binding site for the uncoating ATPase, involved in lattice disassembly By similarity
	Region	493 – 536	44	Flexible linker By similarity
	Region	537 – 1705	1169	Heavy chain arm By similarity
	Region	537 – 648	112	Distal segment By similarity
	Region	653 – 1705	1053	Proximal segment By similarity
	Region	1227 – 1536	310	Involved in binding clathrin light chain By similarity
	Region	1564 – 1705	142	Trimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0WNJ6 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 0850CAE77FE17616	FASTA	1,705	193,245
	10 20 30 40 50 60 MAAANAPIIM KEVLTLPSVG IGQQFITFTN VTMESDKYIC VRETAPQNSV VIIDMNMPMQ 70 80 90 100 110 120 PLRRPITADS ALMNPNSRIL ALKAQVPGTT QDHLQIFNIE AKAKLKSHQM PEQVAFWKWI 130 140 150 160 170 180 TPKMLGLVTQ TSVYHWSIEG DSEPVKMFDR TANLANNQII NYKCSPNEKW LVLIGIAPGS 190 200 210 220 230 240 PERPQLVKGN MQLFSVDQQR SQALEAHAAS FAQFKVPGNE NPSILISFAS KSFNAGQITS 250 260 270 280 290 300 KLHVIELGAQ PGKPSFTKKQ ADLFFPPDFA DDFPVAMQVS HKFNLIYVIT KLGLLFVYDL 310 320 330 340 350 360 ETASAIYRNR ISPDPIFLTS EASSVGGFYA INRRGQVLLA TVNEATIIPF ISGQLNNLEL 370 380 390 400 410 420 AVNLAKRGNL PGAENLVVQR FQELFAQTKY KEAAELAAES PQGILRTPDT VAKFQSVPVQ 430 440 450 460 470 480 AGQTPPLLQY FGTLLTRGKL NSYESLELSR LVVNQNKKNL LENWLAEDKL ECSEELGDLV 490 500 510 520 530 540 KTVDNDLALK IYIKARATPK VVAAFAERRE FDKILIYSKQ VGYTPDYMFL LQTILRTDPQ 550 560 570 580 590 600 GAVNFALMMS QMEGGCPVDY NTITDLFLQR NLIREATAFL LDVLKPNLPE HAFLQTKVLE 610 620 630 640 650 660 INLVTFPNVA DAILANGMFS HYDRPRVAQL CEKAGLYIQS LKHYSELPDI KRVIVNTHAI 670 680 690 700 710 720 EPQALVEFFG TLSSEWAMEC MKDLLLVNLR GNLQIIVQAC KEYCEQLGVD ACIKLFEQFK 730 740 750 760 770 780 SYEGLYFFLG SYLSMSEDPE IHFKYIEAAA KTGQIKEVER VTRESNFYDA EKTKNFLMEA 790 800 810 820 830 840 KLPDARPLIN VCDRFGFVPD LTHYLYTNNM LRYIEGYVQK VNPGNAPLVV GQLLDDECPE 850 860 870 880 890 900 DFIKGLILSV RSLLPVEPLV AECEKRNRLR LLTQFLEHLV SEGSQDVHVH NALGKIIIDS 910 920 930 940 950 960 NNNPEHFLTT NPYYDSKVVG KYCEKRDPTL AVVAYRRGQC DEELINVTNK NSLFKLQARY 970 980 990 1000 1010 1020 VVERMDGDLW EKVLTEENEY RRQLIDQVVS TALPESKSPE QVSAAVKAFM TADLPHELIE 1030 1040 1050 1060 1070 1080 LLEKIVLQNS AFSGNFNLQN LLILTAIKAD PSRVMDYINR LDNFDGPAVG EVAVDAQLYE 1090 1100 1110 1120 1130 1140 EAFAIFKKFN LNVQAVNVLL DNVRSIERAV EFAFRVEEDA VWSQVAKAQL REGLVSDAIE 1150 1160 1170 1180 1190 1200 SFIRADDTTQ FLEVIRASED TNVYDDLVRY LLMVRQKVKE PKVDSELIYA YAKIERLGEI 1210 1220 1230 1240 1250 1260 EEFILMPNVA NLQHVGDRLY DEALYEAAKI IYAFISNWAK LAVTLVKLQQ FQGAVDAARK 1270 1280 1290 1300 1310 1320 ANSAKTWKEV CFACVDAEEF RLAQICGLNI IIQVDDLEEV SEYYQNRGCF NELISLMESG 1330 1340 1350 1360 1370 1380 LGLERAHMGI FTELGVLYAR YRYEKLMEHI KLFSTRLNIP KLIRACDEQQ HWQELTYLYI 1390 1400 1410 1420 1430 1440 QYDEFDNAAT TVMNHSPEAW EHMQFKDIVA KVANVELYYK AVHFYLQEHP DIINDLLNVL 1450 1460 1470 1480 1490 1500 ALRLDHTRVV DIMRKAGHLR LIKPYMVAVQ SNNVSAVNEA LNEIYAEEED YDRLRESIDL 1510 1520 1530 1540 1550 1560 HDSFDQIGLA QKIEKHELVE MRRVAAYIYK KAGRWKQSIA LSKKDNMYKD CMETASQSGD 1570 1580 1590 1600 1610 1620 HDLAEQLLVY FIEQGKKECF ATCLFVCYDL IRPDVALELA WINNMIDFAF PYLLQFIREY 1630 1640 1650 1660 1670 1680 SGKVDELIKD KLEAQKEVKA KEQEEKDVMS QQNMYAQLLP LALPAPPMPG MGGGGYGPPP 1690 1700 QMGGMPGMSG MPPMPPYGMP PMGGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis." Kitakura S., Vanneste S., Robert S., Loefke C., Teichmann T., Tanaka H., Friml J. Plant Cell 23:1920-1931(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC009991 Genomic DNA. Translation: AAF01510.1. Sequence problems. AC073395 Genomic DNA. Translation: AAG50967.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE75005.1. AK229443 mRNA. Translation: BAF01303.1. AK229949 mRNA. Translation: BAF01775.1.
IPI	IPI00527690.
RefSeq	NP_187724.2. NM_111950.2.
UniGene	At.17332. At.26828.
3D structure databases
HSSP	HSSP built from PDB template 1BPO based on UniProtKB P11442.
ProteinModelPortal	Q0WNJ6.
SMR	Q0WNJ6. Positions 1-506, 1091-1643.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q0WNJ6. 2 interactions.
STRING	3702.AT3G11130.1-P.
Proteomic databases
PRIDE	Q0WNJ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G11130.1; AT3G11130.1; AT3G11130.
GeneID	820284.
KEGG	ath:AT3G11130.
Organism-specific databases
TAIR	At3g11130.
Phylogenomic databases
eggNOG	NOG314149.
HOGENOM	HOG000188877.
InParanoid	Q0WNJ6.
KO	K04646.
OMA	MAIRSNL.
PhylomeDB	Q0WNJ6.
ProtClustDB	CLSN2690853.
Gene expression databases
Genevestigator	Q0WNJ6.
Family and domain databases
Gene3D	1.25.40.10. 3 hits. 2.130.10.110. 1 hit.
InterPro	IPR016024. ARM-type_fold. IPR000547. Clathrin_H-chain/VPS_repeat. IPR016025. Clathrin_H-chain_link/propller. IPR015348. Clathrin_H-chain_linker_core. IPR001473. Clathrin_H-chain_propeller_N. IPR022365. Clathrin_H-chain_propeller_rpt. IPR016341. Clathrin_heavy_chain. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF00637. Clathrin. 7 hits. PF09268. Clathrin-link. 1 hit. PF01394. Clathrin_propel. 2 hits. [Graphical view]
PIRSF	PIRSF002290. Clathrin_H_chain. 1 hit.
SMART	SM00299. CLH. 7 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 6 hits. SSF50989. Clathrin_H-chain_propeller_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CLAH1_ARATH

Accession

Primary (citable) accession number: Q0WNJ6
Secondary accession number(s): Q0WM81, Q9SRM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2011
Last sequence update:	September 5, 2006
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program