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Q0WNJ6

- CLAH1_ARATH

UniProt

Q0WNJ6 - CLAH1_ARATH

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Protein
Clathrin heavy chain 1
Gene
CHC1, At3g11130, F11B9.30, F9F8.6
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles By similarity. Mediates endocytosis and is required for a correct polar distribution of PIN auxin transporters.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. structural molecule activity Source: InterPro

GO - Biological processi

  1. endocytosis Source: TAIR
  2. intracellular protein transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin heavy chain 1
Gene namesi
Name:CHC1
Ordered Locus Names:At3g11130
ORF Names:F11B9.30, F9F8.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G11130.

Subcellular locationi

Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Membranecoated pit; Peripheral membrane protein; Cytoplasmic side By similarity
Note: Cytoplasmic face of coated pits and vesicles By similarity.

GO - Cellular componenti

  1. Golgi apparatus Source: TAIR
  2. clathrin coat of coated pit Source: InterPro
  3. clathrin coat of trans-Golgi network vesicle Source: InterPro
  4. plasma membrane Source: TAIR
  5. plasmodesma Source: TAIR
  6. vacuolar membrane Source: TAIR
  7. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 17051704Clathrin heavy chain 1
PRO_0000413949Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ0WNJ6.

Expressioni

Gene expression databases

GenevestigatoriQ0WNJ6.

Interactioni

Subunit structurei

Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
EPSIN1Q8VY073EBI-1162845,EBI-1162785

Protein-protein interaction databases

BioGridi5618. 7 interactions.
IntActiQ0WNJ6. 7 interactions.
STRINGi3702.AT3G11130.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ0WNJ6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati551 – 697147CHCR 1
Add
BLAST
Repeati700 – 842143CHCR 2
Add
BLAST
Repeati847 – 986140CHCR 3
Add
BLAST
Repeati993 – 1138146CHCR 4
Add
BLAST
Repeati1142 – 1283142CHCR 5
Add
BLAST
Repeati1288 – 1434147CHCR 6
Add
BLAST
Repeati1437 – 1580144CHCR 7
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 492491Globular terminal domain By similarity
Add
BLAST
Regioni25 – 6743WD40-like repeat 1
Add
BLAST
Regioni68 – 11346WD40-like repeat 2
Add
BLAST
Regioni114 – 15542WD40-like repeat 3
Add
BLAST
Regioni156 – 20550WD40-like repeat 4
Add
BLAST
Regioni206 – 27065WD40-like repeat 5
Add
BLAST
Regioni271 – 31444WD40-like repeat 6
Add
BLAST
Regioni315 – 34329WD40-like repeat 7
Add
BLAST
Regioni462 – 47817Binding site for the uncoating ATPase, involved in lattice disassembly By similarity
Add
BLAST
Regioni493 – 53644Flexible linker By similarity
Add
BLAST
Regioni537 – 17051169Heavy chain arm By similarity
Add
BLAST
Regioni537 – 648112Distal segment By similarity
Add
BLAST
Regioni653 – 17051053Proximal segment By similarity
Add
BLAST
Regioni1227 – 1536310Involved in binding clathrin light chain By similarity
Add
BLAST
Regioni1564 – 1705142Trimerization By similarity
Add
BLAST

Domaini

The C-terminal third of the heavy chains forms the hub of the triskelion. This region contains the trimerization domain and the light-chain binding domain involved in the assembly of the clathrin lattice.
The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It consitutes a major protein-protein interaction node By similarity.

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG314149.
HOGENOMiHOG000188877.
InParanoidiQ0WNJ6.
KOiK04646.
OMAiLAGCQMI.
PhylomeDBiQ0WNJ6.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
2.130.10.110. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016025. Clathrin_H-chain_link/propller.
IPR015348. Clathrin_H-chain_linker_core.
IPR001473. Clathrin_H-chain_propeller_N.
IPR022365. Clathrin_H-chain_propeller_rpt.
IPR016341. Clathrin_heavy_chain.
IPR011990. TPR-like_helical.
[Graphical view]
PfamiPF00637. Clathrin. 7 hits.
PF09268. Clathrin-link. 1 hit.
PF01394. Clathrin_propel. 2 hits.
[Graphical view]
PIRSFiPIRSF002290. Clathrin_H_chain. 1 hit.
SMARTiSM00299. CLH. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
SSF50989. SSF50989. 1 hit.
PROSITEiPS50236. CHCR. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0WNJ6-1 [UniParc]FASTAAdd to Basket

« Hide

MAAANAPIIM KEVLTLPSVG IGQQFITFTN VTMESDKYIC VRETAPQNSV     50
VIIDMNMPMQ PLRRPITADS ALMNPNSRIL ALKAQVPGTT QDHLQIFNIE 100
AKAKLKSHQM PEQVAFWKWI TPKMLGLVTQ TSVYHWSIEG DSEPVKMFDR 150
TANLANNQII NYKCSPNEKW LVLIGIAPGS PERPQLVKGN MQLFSVDQQR 200
SQALEAHAAS FAQFKVPGNE NPSILISFAS KSFNAGQITS KLHVIELGAQ 250
PGKPSFTKKQ ADLFFPPDFA DDFPVAMQVS HKFNLIYVIT KLGLLFVYDL 300
ETASAIYRNR ISPDPIFLTS EASSVGGFYA INRRGQVLLA TVNEATIIPF 350
ISGQLNNLEL AVNLAKRGNL PGAENLVVQR FQELFAQTKY KEAAELAAES 400
PQGILRTPDT VAKFQSVPVQ AGQTPPLLQY FGTLLTRGKL NSYESLELSR 450
LVVNQNKKNL LENWLAEDKL ECSEELGDLV KTVDNDLALK IYIKARATPK 500
VVAAFAERRE FDKILIYSKQ VGYTPDYMFL LQTILRTDPQ GAVNFALMMS 550
QMEGGCPVDY NTITDLFLQR NLIREATAFL LDVLKPNLPE HAFLQTKVLE 600
INLVTFPNVA DAILANGMFS HYDRPRVAQL CEKAGLYIQS LKHYSELPDI 650
KRVIVNTHAI EPQALVEFFG TLSSEWAMEC MKDLLLVNLR GNLQIIVQAC 700
KEYCEQLGVD ACIKLFEQFK SYEGLYFFLG SYLSMSEDPE IHFKYIEAAA 750
KTGQIKEVER VTRESNFYDA EKTKNFLMEA KLPDARPLIN VCDRFGFVPD 800
LTHYLYTNNM LRYIEGYVQK VNPGNAPLVV GQLLDDECPE DFIKGLILSV 850
RSLLPVEPLV AECEKRNRLR LLTQFLEHLV SEGSQDVHVH NALGKIIIDS 900
NNNPEHFLTT NPYYDSKVVG KYCEKRDPTL AVVAYRRGQC DEELINVTNK 950
NSLFKLQARY VVERMDGDLW EKVLTEENEY RRQLIDQVVS TALPESKSPE 1000
QVSAAVKAFM TADLPHELIE LLEKIVLQNS AFSGNFNLQN LLILTAIKAD 1050
PSRVMDYINR LDNFDGPAVG EVAVDAQLYE EAFAIFKKFN LNVQAVNVLL 1100
DNVRSIERAV EFAFRVEEDA VWSQVAKAQL REGLVSDAIE SFIRADDTTQ 1150
FLEVIRASED TNVYDDLVRY LLMVRQKVKE PKVDSELIYA YAKIERLGEI 1200
EEFILMPNVA NLQHVGDRLY DEALYEAAKI IYAFISNWAK LAVTLVKLQQ 1250
FQGAVDAARK ANSAKTWKEV CFACVDAEEF RLAQICGLNI IIQVDDLEEV 1300
SEYYQNRGCF NELISLMESG LGLERAHMGI FTELGVLYAR YRYEKLMEHI 1350
KLFSTRLNIP KLIRACDEQQ HWQELTYLYI QYDEFDNAAT TVMNHSPEAW 1400
EHMQFKDIVA KVANVELYYK AVHFYLQEHP DIINDLLNVL ALRLDHTRVV 1450
DIMRKAGHLR LIKPYMVAVQ SNNVSAVNEA LNEIYAEEED YDRLRESIDL 1500
HDSFDQIGLA QKIEKHELVE MRRVAAYIYK KAGRWKQSIA LSKKDNMYKD 1550
CMETASQSGD HDLAEQLLVY FIEQGKKECF ATCLFVCYDL IRPDVALELA 1600
WINNMIDFAF PYLLQFIREY SGKVDELIKD KLEAQKEVKA KEQEEKDVMS 1650
QQNMYAQLLP LALPAPPMPG MGGGGYGPPP QMGGMPGMSG MPPMPPYGMP 1700
PMGGY 1705
Length:1,705
Mass (Da):193,245
Last modified:September 5, 2006 - v1
Checksum:i0850CAE77FE17616
GO

Sequence cautioni

The sequence AAF01510.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence AAG50967.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC009991 Genomic DNA. Translation: AAF01510.1. Sequence problems.
AC073395 Genomic DNA. Translation: AAG50967.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75005.1.
AK229443 mRNA. Translation: BAF01303.1.
AK229949 mRNA. Translation: BAF01775.1.
RefSeqiNP_187724.2. NM_111950.2.
UniGeneiAt.17332.
At.26828.

Genome annotation databases

EnsemblPlantsiAT3G11130.1; AT3G11130.1; AT3G11130.
GeneIDi820284.
KEGGiath:AT3G11130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC009991 Genomic DNA. Translation: AAF01510.1 . Sequence problems.
AC073395 Genomic DNA. Translation: AAG50967.1 . Sequence problems.
CP002686 Genomic DNA. Translation: AEE75005.1 .
AK229443 mRNA. Translation: BAF01303.1 .
AK229949 mRNA. Translation: BAF01775.1 .
RefSeqi NP_187724.2. NM_111950.2.
UniGenei At.17332.
At.26828.

3D structure databases

ProteinModelPortali Q0WNJ6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 5618. 7 interactions.
IntActi Q0WNJ6. 7 interactions.
STRINGi 3702.AT3G11130.1-P.

Proteomic databases

PRIDEi Q0WNJ6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G11130.1 ; AT3G11130.1 ; AT3G11130 .
GeneIDi 820284.
KEGGi ath:AT3G11130.

Organism-specific databases

TAIRi AT3G11130.

Phylogenomic databases

eggNOGi NOG314149.
HOGENOMi HOG000188877.
InParanoidi Q0WNJ6.
KOi K04646.
OMAi LAGCQMI.
PhylomeDBi Q0WNJ6.

Gene expression databases

Genevestigatori Q0WNJ6.

Family and domain databases

Gene3Di 1.25.40.10. 3 hits.
2.130.10.110. 1 hit.
InterProi IPR016024. ARM-type_fold.
IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016025. Clathrin_H-chain_link/propller.
IPR015348. Clathrin_H-chain_linker_core.
IPR001473. Clathrin_H-chain_propeller_N.
IPR022365. Clathrin_H-chain_propeller_rpt.
IPR016341. Clathrin_heavy_chain.
IPR011990. TPR-like_helical.
[Graphical view ]
Pfami PF00637. Clathrin. 7 hits.
PF09268. Clathrin-link. 1 hit.
PF01394. Clathrin_propel. 2 hits.
[Graphical view ]
PIRSFi PIRSF002290. Clathrin_H_chain. 1 hit.
SMARTi SM00299. CLH. 7 hits.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 5 hits.
SSF50989. SSF50989. 1 hit.
PROSITEi PS50236. CHCR. 7 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis."
    Kitakura S., Vanneste S., Robert S., Loefke C., Teichmann T., Tanaka H., Friml J.
    Plant Cell 23:1920-1931(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCLAH1_ARATH
AccessioniPrimary (citable) accession number: Q0WNJ6
Secondary accession number(s): Q0WM81, Q9SRM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: September 5, 2006
Last modified: September 3, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi