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Q0WNJ6

- CLAH1_ARATH

UniProt

Q0WNJ6 - CLAH1_ARATH

Protein

Clathrin heavy chain 1

Gene

CHC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Clathrin is the major protein of the polyhedral coat of coated pits and vesicles By similarity. Mediates endocytosis and is required for a correct polar distribution of PIN auxin transporters.By similarity1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. structural molecule activity Source: InterPro

    GO - Biological processi

    1. endocytosis Source: TAIR
    2. intracellular protein transport Source: InterPro

    Keywords - Biological processi

    Endocytosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Clathrin heavy chain 1
    Gene namesi
    Name:CHC1
    Ordered Locus Names:At3g11130
    ORF Names:F11B9.30, F9F8.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G11130.

    Subcellular locationi

    Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Membranecoated pit By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: Cytoplasmic face of coated pits and vesicles.By similarity

    GO - Cellular componenti

    1. clathrin coat of coated pit Source: InterPro
    2. clathrin coat of trans-Golgi network vesicle Source: InterPro
    3. Golgi apparatus Source: TAIR
    4. plasma membrane Source: TAIR
    5. plasmodesma Source: TAIR
    6. vacuolar membrane Source: TAIR
    7. vacuole Source: TAIR

    Keywords - Cellular componenti

    Coated pit, Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 17051704Clathrin heavy chain 1PRO_0000413949Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ0WNJ6.

    Expressioni

    Gene expression databases

    GenevestigatoriQ0WNJ6.

    Interactioni

    Subunit structurei

    Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EPSIN1Q8VY073EBI-1162845,EBI-1162785

    Protein-protein interaction databases

    BioGridi5618. 7 interactions.
    IntActiQ0WNJ6. 7 interactions.
    STRINGi3702.AT3G11130.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0WNJ6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati551 – 697147CHCR 1Add
    BLAST
    Repeati700 – 842143CHCR 2Add
    BLAST
    Repeati847 – 986140CHCR 3Add
    BLAST
    Repeati993 – 1138146CHCR 4Add
    BLAST
    Repeati1142 – 1283142CHCR 5Add
    BLAST
    Repeati1288 – 1434147CHCR 6Add
    BLAST
    Repeati1437 – 1580144CHCR 7Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 492491Globular terminal domainBy similarityAdd
    BLAST
    Regioni25 – 6743WD40-like repeat 1Add
    BLAST
    Regioni68 – 11346WD40-like repeat 2Add
    BLAST
    Regioni114 – 15542WD40-like repeat 3Add
    BLAST
    Regioni156 – 20550WD40-like repeat 4Add
    BLAST
    Regioni206 – 27065WD40-like repeat 5Add
    BLAST
    Regioni271 – 31444WD40-like repeat 6Add
    BLAST
    Regioni315 – 34329WD40-like repeat 7Add
    BLAST
    Regioni462 – 47817Binding site for the uncoating ATPase, involved in lattice disassemblyBy similarityAdd
    BLAST
    Regioni493 – 53644Flexible linkerBy similarityAdd
    BLAST
    Regioni537 – 17051169Heavy chain armBy similarityAdd
    BLAST
    Regioni537 – 648112Distal segmentBy similarityAdd
    BLAST
    Regioni653 – 17051053Proximal segmentBy similarityAdd
    BLAST
    Regioni1227 – 1536310Involved in binding clathrin light chainBy similarityAdd
    BLAST
    Regioni1564 – 1705142TrimerizationBy similarityAdd
    BLAST

    Domaini

    The C-terminal third of the heavy chains forms the hub of the triskelion. This region contains the trimerization domain and the light-chain binding domain involved in the assembly of the clathrin lattice.
    The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It consitutes a major protein-protein interaction node By similarity.By similarity

    Sequence similaritiesi

    Belongs to the clathrin heavy chain family.Curated
    Contains 7 CHCR (clathrin heavy-chain) repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG314149.
    HOGENOMiHOG000188877.
    InParanoidiQ0WNJ6.
    KOiK04646.
    OMAiLAGCQMI.
    PhylomeDBiQ0WNJ6.

    Family and domain databases

    Gene3Di1.25.40.10. 3 hits.
    2.130.10.110. 1 hit.
    InterProiIPR016024. ARM-type_fold.
    IPR000547. Clathrin_H-chain/VPS_repeat.
    IPR016025. Clathrin_H-chain_link/propller.
    IPR015348. Clathrin_H-chain_linker_core.
    IPR001473. Clathrin_H-chain_propeller_N.
    IPR022365. Clathrin_H-chain_propeller_rpt.
    IPR016341. Clathrin_heavy_chain.
    IPR011990. TPR-like_helical.
    [Graphical view]
    PfamiPF00637. Clathrin. 7 hits.
    PF09268. Clathrin-link. 1 hit.
    PF01394. Clathrin_propel. 2 hits.
    [Graphical view]
    PIRSFiPIRSF002290. Clathrin_H_chain. 1 hit.
    SMARTiSM00299. CLH. 7 hits.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 5 hits.
    SSF50989. SSF50989. 1 hit.
    PROSITEiPS50236. CHCR. 7 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q0WNJ6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAANAPIIM KEVLTLPSVG IGQQFITFTN VTMESDKYIC VRETAPQNSV     50
    VIIDMNMPMQ PLRRPITADS ALMNPNSRIL ALKAQVPGTT QDHLQIFNIE 100
    AKAKLKSHQM PEQVAFWKWI TPKMLGLVTQ TSVYHWSIEG DSEPVKMFDR 150
    TANLANNQII NYKCSPNEKW LVLIGIAPGS PERPQLVKGN MQLFSVDQQR 200
    SQALEAHAAS FAQFKVPGNE NPSILISFAS KSFNAGQITS KLHVIELGAQ 250
    PGKPSFTKKQ ADLFFPPDFA DDFPVAMQVS HKFNLIYVIT KLGLLFVYDL 300
    ETASAIYRNR ISPDPIFLTS EASSVGGFYA INRRGQVLLA TVNEATIIPF 350
    ISGQLNNLEL AVNLAKRGNL PGAENLVVQR FQELFAQTKY KEAAELAAES 400
    PQGILRTPDT VAKFQSVPVQ AGQTPPLLQY FGTLLTRGKL NSYESLELSR 450
    LVVNQNKKNL LENWLAEDKL ECSEELGDLV KTVDNDLALK IYIKARATPK 500
    VVAAFAERRE FDKILIYSKQ VGYTPDYMFL LQTILRTDPQ GAVNFALMMS 550
    QMEGGCPVDY NTITDLFLQR NLIREATAFL LDVLKPNLPE HAFLQTKVLE 600
    INLVTFPNVA DAILANGMFS HYDRPRVAQL CEKAGLYIQS LKHYSELPDI 650
    KRVIVNTHAI EPQALVEFFG TLSSEWAMEC MKDLLLVNLR GNLQIIVQAC 700
    KEYCEQLGVD ACIKLFEQFK SYEGLYFFLG SYLSMSEDPE IHFKYIEAAA 750
    KTGQIKEVER VTRESNFYDA EKTKNFLMEA KLPDARPLIN VCDRFGFVPD 800
    LTHYLYTNNM LRYIEGYVQK VNPGNAPLVV GQLLDDECPE DFIKGLILSV 850
    RSLLPVEPLV AECEKRNRLR LLTQFLEHLV SEGSQDVHVH NALGKIIIDS 900
    NNNPEHFLTT NPYYDSKVVG KYCEKRDPTL AVVAYRRGQC DEELINVTNK 950
    NSLFKLQARY VVERMDGDLW EKVLTEENEY RRQLIDQVVS TALPESKSPE 1000
    QVSAAVKAFM TADLPHELIE LLEKIVLQNS AFSGNFNLQN LLILTAIKAD 1050
    PSRVMDYINR LDNFDGPAVG EVAVDAQLYE EAFAIFKKFN LNVQAVNVLL 1100
    DNVRSIERAV EFAFRVEEDA VWSQVAKAQL REGLVSDAIE SFIRADDTTQ 1150
    FLEVIRASED TNVYDDLVRY LLMVRQKVKE PKVDSELIYA YAKIERLGEI 1200
    EEFILMPNVA NLQHVGDRLY DEALYEAAKI IYAFISNWAK LAVTLVKLQQ 1250
    FQGAVDAARK ANSAKTWKEV CFACVDAEEF RLAQICGLNI IIQVDDLEEV 1300
    SEYYQNRGCF NELISLMESG LGLERAHMGI FTELGVLYAR YRYEKLMEHI 1350
    KLFSTRLNIP KLIRACDEQQ HWQELTYLYI QYDEFDNAAT TVMNHSPEAW 1400
    EHMQFKDIVA KVANVELYYK AVHFYLQEHP DIINDLLNVL ALRLDHTRVV 1450
    DIMRKAGHLR LIKPYMVAVQ SNNVSAVNEA LNEIYAEEED YDRLRESIDL 1500
    HDSFDQIGLA QKIEKHELVE MRRVAAYIYK KAGRWKQSIA LSKKDNMYKD 1550
    CMETASQSGD HDLAEQLLVY FIEQGKKECF ATCLFVCYDL IRPDVALELA 1600
    WINNMIDFAF PYLLQFIREY SGKVDELIKD KLEAQKEVKA KEQEEKDVMS 1650
    QQNMYAQLLP LALPAPPMPG MGGGGYGPPP QMGGMPGMSG MPPMPPYGMP 1700
    PMGGY 1705
    Length:1,705
    Mass (Da):193,245
    Last modified:September 5, 2006 - v1
    Checksum:i0850CAE77FE17616
    GO

    Sequence cautioni

    The sequence AAF01510.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence AAG50967.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC009991 Genomic DNA. Translation: AAF01510.1. Sequence problems.
    AC073395 Genomic DNA. Translation: AAG50967.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75005.1.
    AK229443 mRNA. Translation: BAF01303.1.
    AK229949 mRNA. Translation: BAF01775.1.
    RefSeqiNP_187724.2. NM_111950.2.
    UniGeneiAt.17332.
    At.26828.

    Genome annotation databases

    EnsemblPlantsiAT3G11130.1; AT3G11130.1; AT3G11130.
    GeneIDi820284.
    KEGGiath:AT3G11130.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC009991 Genomic DNA. Translation: AAF01510.1 . Sequence problems.
    AC073395 Genomic DNA. Translation: AAG50967.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75005.1 .
    AK229443 mRNA. Translation: BAF01303.1 .
    AK229949 mRNA. Translation: BAF01775.1 .
    RefSeqi NP_187724.2. NM_111950.2.
    UniGenei At.17332.
    At.26828.

    3D structure databases

    ProteinModelPortali Q0WNJ6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 5618. 7 interactions.
    IntActi Q0WNJ6. 7 interactions.
    STRINGi 3702.AT3G11130.1-P.

    Proteomic databases

    PRIDEi Q0WNJ6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G11130.1 ; AT3G11130.1 ; AT3G11130 .
    GeneIDi 820284.
    KEGGi ath:AT3G11130.

    Organism-specific databases

    TAIRi AT3G11130.

    Phylogenomic databases

    eggNOGi NOG314149.
    HOGENOMi HOG000188877.
    InParanoidi Q0WNJ6.
    KOi K04646.
    OMAi LAGCQMI.
    PhylomeDBi Q0WNJ6.

    Gene expression databases

    Genevestigatori Q0WNJ6.

    Family and domain databases

    Gene3Di 1.25.40.10. 3 hits.
    2.130.10.110. 1 hit.
    InterProi IPR016024. ARM-type_fold.
    IPR000547. Clathrin_H-chain/VPS_repeat.
    IPR016025. Clathrin_H-chain_link/propller.
    IPR015348. Clathrin_H-chain_linker_core.
    IPR001473. Clathrin_H-chain_propeller_N.
    IPR022365. Clathrin_H-chain_propeller_rpt.
    IPR016341. Clathrin_heavy_chain.
    IPR011990. TPR-like_helical.
    [Graphical view ]
    Pfami PF00637. Clathrin. 7 hits.
    PF09268. Clathrin-link. 1 hit.
    PF01394. Clathrin_propel. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF002290. Clathrin_H_chain. 1 hit.
    SMARTi SM00299. CLH. 7 hits.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 5 hits.
    SSF50989. SSF50989. 1 hit.
    PROSITEi PS50236. CHCR. 7 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis."
      Kitakura S., Vanneste S., Robert S., Loefke C., Teichmann T., Tanaka H., Friml J.
      Plant Cell 23:1920-1931(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCLAH1_ARATH
    AccessioniPrimary (citable) accession number: Q0WNJ6
    Secondary accession number(s): Q0WM81, Q9SRM1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2011
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3