Q0WML0 (AB27B_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ABC transporter B family member 27
Short name=ABC transporter ABCB.27
Short name=AtABCB27

Alternative name(s):
Aluminum tolerance-related ATP-binding cassette transporter
Antigen peptide transporter-like 2
Transporter associated with antigen processing-like protein 2
Short name=AtTAP2

Gene names

Name:	ABCB27
Synonyms:	ALS1, TAP2
Ordered Locus Names:	At5g39040
ORF Names:	MXF12.50

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	644 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in redistribution of internalized aluminum. May mediate vacuolar sequestration of a metal complex. Ref.2
Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.2 Ref.6.
Tissue specificity	Expressed in the vascular tissue of roots, leaves, stems and flowers, in the distal portion of the root tip, in leaf hyathodes and in anther filaments, pistils and flower receptacles. Ref.2
Induction	Not induces by aluminum treatment. Ref.2
Sequence similarities	Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification] Contains 1 ABC transmembrane type-1 domain. Contains 1 ABC transporter domain.
Sequence caution	The sequence BAB10828.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to aluminum ion Inferred from mutant phenotype. Source: TAIR
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plant-type vacuole Inferred from direct assay Ref.6. Source: TAIR vacuolar membrane Inferred from direct assay Ref.6. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of substances Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 644	644	ABC transporter B family member 27		PRO_0000300103
Regions
	Transmembrane	121 – 141	21	Helical; Potential
	Transmembrane	207 – 227	21	Helical; Potential
	Transmembrane	298 – 318	21	Helical; Potential
	Transmembrane	327 – 347	21	Helical; Potential
	Domain	71 – 362	292	ABC transmembrane type-1
	Domain	396 – 633	238	ABC transporter
	Nucleotide binding	431 – 438	8	ATP Potential
	Compositional bias	637 – 640	4	Poly-Ser
Experimental info
	Mutagenesis	261	1	E → K in als1-1; loss of aluminum tolerence. Ref.2
	Sequence conflict	47	1	H → Y in AAL85486. Ref.1
	Sequence conflict	504	1	D → N in AAL85486. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q0WML0 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 5A29C0CF8AC19884	FASTA	644	69,104
	10 20 30 40 50 60 MGNKKLLTGG SSKTHGSGSS YRDPLLQNQE DKPKANGSEN GLNDLEHGVV EAANVGFGRV 70 80 90 100 110 120 FALAKPDAGK LVIGTIALLI GSTTNLLVPK FGGMIIDIVS RDVKTPEQQT ESLIAVRNAV 130 140 150 160 170 180 VIILLIVVIG SICTALRAWL FNSASERVVA RLRKDLFRHL MHQEIAFYDV TKTGELLSRL 190 200 210 220 230 240 SEDTQIIKNA ATTNLSEALR NVTTALIGVG FMFTSSWKLT LLALVVVPVI SVAVKQFGRY 250 260 270 280 290 300 LRELSHTTQA AAAVAASIAE ESFGAVRTVR SFAKESYMVS QYSKKVDETL KLGLKQAVLV 310 320 330 340 350 360 GLFFGGLNAA FTLSVITVVS YGAYLTIYGS MTVGALTSFI LYSLTVGSSV SSLSSLYTTA 370 380 390 400 410 420 MKAAGASRRV FQILDRVSSM SSSGDKCPVG NPDGDVELND VWFAYPSRPS HMILKGISLR 430 440 450 460 470 480 LTPGSKVALV GPSGGGKTTI ANLIERFYDP LKGKILLNGV SLMEISHQYL HKQISIVSQE 490 500 510 520 530 540 PILFNCSVEE NIAYGFDGEA SFTDIENAAK MANAHEFIEA FPDKYNTVVG ERGLRLSGGQ 550 560 570 580 590 600 KQRIAIARAL LTNPSVLLLD EATSALDAES EYLVQDAMDS LMAGRTVLVI AHRLSTVKTA 610 620 630 640 DCVAVISDGE VAEKGTHDEL LSLNGIYTNL VKRQLQSSSS VTTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Arabidopsis thaliana ABC protein superfamily, a complete inventory." Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A. J. Biol. Chem. 276:30231-30244(2001) [PubMed: 11346655] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
[2]	"Arabidopsis ALS1 encodes a root tip and stele localized half type ABC transporter required for root growth in an aluminum toxic environment." Larsen P.B., Cancel J., Rounds M., Ochoa V. Planta 225:1447-1458(2007) [PubMed: 17171374] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF GLU-261, LACK OF INDUCTION BY ALUMINUM, FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones." Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S. DNA Res. 5:379-391(1998) [PubMed: 10048488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Mapping the Arabidopsis organelle proteome." Dunkley T.P.J., Hester S., Shadforth I.P., Runions J., Weimar T., Hanton S.L., Griffin J.L., Bessant C., Brandizzi F., Hawes C., Watson R.B., Dupree P., Lilley K.S. Proc. Natl. Acad. Sci. U.S.A. 103:6518-6523(2006) [PubMed: 16618929] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
[7]	"Plant ABC proteins - a unified nomenclature and updated inventory." Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M., Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A., Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L. Trends Plant Sci. 13:151-159(2008) [PubMed: 18299247] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY078410 mRNA. Translation: AAL85486.1. DQ469798 mRNA. Translation: ABG37923.1. AB016892 Genomic DNA. Translation: BAB10828.1. Sequence problems. CP002688 Genomic DNA. Translation: AED94390.1. AK229810 mRNA. Translation: BAF01641.1.
IPI	IPI00538470.
RefSeq	NP_198720.2. NM_123266.7.
UniGene	At.9429.
3D structure databases
ProteinModelPortal	Q0WML0.
SMR	Q0WML0. Positions 55-636.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0WML0.
Protein family/group databases
TCDB	3.A.1.201.8. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PRIDE	Q0WML0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G39040.1; AT5G39040.1; AT5G39040.
GeneID	833896.
GenomeReviews	Gene locus AT5G39040 in contig BA000015_GR.
KEGG	ath:AT5G39040.
NMPDR	fig|3702.1.peg.25601.
Organism-specific databases
TAIR	At5g39040.
Phylogenomic databases
eggNOG	KOG0058.
GeneTree	EPGT00070000028613.
HOGENOM	HBG758042.
InParanoid	Q0WML0.
OMA	VELNDVW.
PhylomeDB	Q0WML0.
ProtClustDB	CLSN2690214.
Gene expression databases
Genevestigator	Q0WML0.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 1 hit. PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 1 hit.
PROSITE	PS50929. ABC_TM1F. 1 hit. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AB27B_ARATH

Accession

Primary (citable) accession number: Q0WML0
Secondary accession number(s): Q8RY45, Q9FID4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 5, 2006
Last modified:	November 16, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program