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Reviewed, UniProtKB/Swiss-Prot Q0WM29 (MMSA_ARATH)

Last modified February 9, 2010. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
      Short name=Malonate-semialdehyde dehydrogenase [acylating]
      Short name=MMSDH
      Short name=MM-ALDH
    EC=1.2.1.27
Alternative name(s):
    Aldehyde dehydrogenase family 6 member B2
Gene names
Name: ALDH6B2
Ordered Locus Names: At2g14170
ORF Names: F15N24.6, T22C12.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length607 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH.

Subcellular location

Mitochondrion Potential.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Caution

In contrast to other members of the family, the conserved Glu residue in position 94 is replaced by an Arg. Its activity is therefore unsure.

Ontologies

Keywords
   Cellular componentMitochondrion
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

response to oxidative stress

Inferred from direct assay. Source: TAIR

valine metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentmitochondrion

Inferred from direct assay. Source: TAIR

   Molecular functionmethylmalonate-semialdehyde dehydrogenase (acylating) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q0WM29-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2424Mitochondrion Potential
Chain25 – 607583Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
PRO_0000256067

Regions

Nucleotide binding335 – 3406NAD By similarity

Sites

Active site3911Nucleophile By similarity
Site2601Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 31, 2006. Version 2.
Checksum: 0257A3606B31165C

FASTA60765,927
        10         20         30         40         50         60 
MVRVKQKNLE SYRSNGTYPP TWRNPTTSFA PDQHRVSIHS SLKSKTKRRR LYKEADDNTK 

        70         80         90        100        110        120 
LRSSSSTTTT TTTMLLRISG NNLRPLRPQF LALRSSWLST SPEQSTQPQM PPRVPNLIGG 

       130        140        150        160        170        180 
SFVESQSSSF IDVINPATQE VVSKVPLTTN EEFKAAVSAA KQAFPLWRNT PITTRQRVML 

       190        200        210        220        230        240 
KFQELIRKNM DKLAMNITTE QGKTLKDSHG DIFRGLEVVE HACGMATLQM GEYLPNVSNG 

       250        260        270        280        290        300 
VDTYSIREPL GVCAGICPFN FPAMIPLWMF PVAVTCGNTF ILKPSEKDPG ASVILAELAM 

       310        320        330        340        350        360 
EAGLPDGVLN IVHGTNDTVN AICDDEDIRA VSFVGSNTAG MHIYARAAAK GKRIQSNMGA 

       370        380        390        400        410        420 
KNHGLVLPDA NIDATLNALL AAGFGAAGQR CMALSTVVFV GDAKSWEDKL VERAKALKVT 

       430        440        450        460        470        480 
CGSEPDADLG PVISKQAKER ICRLIQSGVD DGAKLLLDGR DIVVPGYEKG NFIGPTILSG 

       490        500        510        520        530        540 
VTPDMECYKE EIFGPVLVCM QANSFDEAIS IINKNKYGNG AAIFTSSGAA ARKFQMDIEA 

       550        560        570        580        590        600 
GQIGINVPIP VPLPFFSFTG NKASFAGDLN FYGKAGVDFF TQIKTVTQQW KDIPTSVSLA 


MPTSQKQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC007197 Genomic DNA. Translation: AAD25855.1.
AK230004 mRNA. Translation: BAF01828.1.
IPIIPI00518167.
PIRH84514.
RefSeqNP_179032.1.
UniGeneAt.43851
Rra.7414
Rsa.5843

3D structure databases

SMRQ0WM29. Positions 112-591.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0WM29.

Proteomic databases

PRIDEQ0WM29.

Genome annotation databases

GeneID815903.
GenomeReviewsGene locus AT2G14170 in contig CT485783_GR.
NMPDRfig|3702.1.peg.8445.

Organism-specific databases

GeneFarm4385.
TAIRAt2g14170.

Phylogenomic databases

HOGENOMHBG752218.
InParanoidQ0WM29.
OMAIISNVKP.
PhylomeDBQ0WM29.

Enzyme and pathway databases

BRENDA1.2.1.27. 302.

Gene expression databases

GenevestigatorQ0WM29.

Family and domain databases

InterProIPR016161. Ald_DH/histidinol_DH.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH.
IPR010061. MeMal-semiAld_DH.
[Graphical view]
Gene3DG3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHERPTHR11699. Aldehyde_dehyd. 1 hit.
PTHR11699:SF27. MMSDH. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
TIGRFAMsTIGR01722. MMSDH. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMMSA_ARATH
AccessionPrimary (citable) accession number: Q0WM29
Secondary accession number(s): Q9SI43
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: February 9, 2010
This is version 32 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents