Reviewed, UniProtKB/Swiss-Prot Q0WM29 (MMSA_ARATH)

Names and origin

Protein names

Recommended name:
    Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
      Short name=MMSDH
      Short name=MM-ALDH
    EC=1.2.1.27
Alternative name(s):
    Aldehyde dehydrogenase family 6 member B2

Gene names

Name:	ALDH6B2
Ordered Locus Names:	At2g14170
ORF Names:	F15N24.6, T22C12.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	2-methyl-3-oxopropanoate + CoA + H(2)O + NAD(+) = propanoyl-CoA + HCO(3)(-) + NADH.
Subcellular location	MitochondrionPotential.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.
Caution	In contrast to other members of the family, the conserved Glu residue in position 94 is replaced by an Arg. Its activity is therefore unsure.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Notes: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q0WM29-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion Potential
	Chain	25 – 607	583	Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial		PRO_0000256067
Regions
	Nucleotide binding	335 – 340	6	NAD By similarity
Sites
	Active site	391	1	Nucleophile By similarity
	Site	260	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 0257A3606B31165C	FASTA	607	65,927
	10 20 30 40 50 60 MVRVKQKNLE SYRSNGTYPP TWRNPTTSFA PDQHRVSIHS SLKSKTKRRR LYKEADDNTK 70 80 90 100 110 120 LRSSSSTTTT TTTMLLRISG NNLRPLRPQF LALRSSWLST SPEQSTQPQM PPRVPNLIGG 130 140 150 160 170 180 SFVESQSSSF IDVINPATQE VVSKVPLTTN EEFKAAVSAA KQAFPLWRNT PITTRQRVML 190 200 210 220 230 240 KFQELIRKNM DKLAMNITTE QGKTLKDSHG DIFRGLEVVE HACGMATLQM GEYLPNVSNG 250 260 270 280 290 300 VDTYSIREPL GVCAGICPFN FPAMIPLWMF PVAVTCGNTF ILKPSEKDPG ASVILAELAM 310 320 330 340 350 360 EAGLPDGVLN IVHGTNDTVN AICDDEDIRA VSFVGSNTAG MHIYARAAAK GKRIQSNMGA 370 380 390 400 410 420 KNHGLVLPDA NIDATLNALL AAGFGAAGQR CMALSTVVFV GDAKSWEDKL VERAKALKVT 430 440 450 460 470 480 CGSEPDADLG PVISKQAKER ICRLIQSGVD DGAKLLLDGR DIVVPGYEKG NFIGPTILSG 490 500 510 520 530 540 VTPDMECYKE EIFGPVLVCM QANSFDEAIS IINKNKYGNG AAIFTSSGAA ARKFQMDIEA 550 560 570 580 590 600 GQIGINVPIP VPLPFFSFTG NKASFAGDLN FYGKAGVDFF TQIKTVTQQW KDIPTSVSLA MPTSQKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 294-607. Strain: cv. Columbia.
[3]	"The ALDH gene superfamily of Arabidopsis." Kirch H.-H., Bartels D., Wei Y., Schnable P.S., Wood A.J. Trends Plant Sci. 9:371-377(2004) [PubMed: 15358267] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC007197 Genomic DNA. Translation: AAD25855.1. AK230004 mRNA. Translation: BAF01828.1.
PIR	H84514.
RefSeq	NP_179032.1.
UniGene	At.43851
3D structure databases
HSSP	HSSP built from PDB template 1BXS based on UniProtKB P51977.
ModBase	Search...
Genome annotation databases
GeneID	815903.
GenomeReviews	Gene locus AT2G14170 in contig CT485783_GR.
NMPDR	fig|3702.1.peg.8445.
Organism-specific databases
GeneFarm	4385.
TAIR	At2g14170.
Family and domain databases
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR010061. MeMal-semiAld_DHase. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MMSA_ARATH

Accession

Primary (citable) accession number: Q0WM29
Secondary accession number(s): Q9SI43

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	November 25, 2008
This is version 20 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)