Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial precursor

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Reviewed, UniProtKB/Swiss-Prot Q0WM29 (MMSA_ARATH)

Last modified February 9, 2010. Version 32. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
      Short name=Malonate-semialdehyde dehydrogenase [acylating]
      Short name=MMSDH
      Short name=MM-ALDH
    EC=1.2.1.27
Alternative name(s):
    Aldehyde dehydrogenase family 6 member B2

Gene names

Name:	ALDH6B2
Ordered Locus Names:	At2g14170
ORF Names:	F15N24.6, T22C12.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Catalytic activity	2-methyl-3-oxopropanoate + CoA + H₂O + NAD⁺ = propanoyl-CoA + HCO₃^- + NADH.
Subcellular location	Mitochondrion Potential.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.
Caution	In contrast to other members of the family, the conserved Glu residue in position 94 is replaced by an Arg. Its activity is therefore unsure.

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Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from direct assay. Source: TAIR valine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: TAIR
Molecular function	methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q0WM29-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 24

Mitochondrion Potential

Chain

25 – 607

583

Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial

PRO_0000256067

Regions

Nucleotide binding

335 – 340

NAD By similarity

Sites

Active site

391

Nucleophile By similarity

Site

260

Transition state stabilizer By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified October 31, 2006. Version 2.
Checksum: 0257A3606B31165C
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FASTA

607

65,927

        10         20         30         40         50         60 
MVRVKQKNLE SYRSNGTYPP TWRNPTTSFA PDQHRVSIHS SLKSKTKRRR LYKEADDNTK 

        70         80         90        100        110        120 
LRSSSSTTTT TTTMLLRISG NNLRPLRPQF LALRSSWLST SPEQSTQPQM PPRVPNLIGG 

       130        140        150        160        170        180 
SFVESQSSSF IDVINPATQE VVSKVPLTTN EEFKAAVSAA KQAFPLWRNT PITTRQRVML 

       190        200        210        220        230        240 
KFQELIRKNM DKLAMNITTE QGKTLKDSHG DIFRGLEVVE HACGMATLQM GEYLPNVSNG 

       250        260        270        280        290        300 
VDTYSIREPL GVCAGICPFN FPAMIPLWMF PVAVTCGNTF ILKPSEKDPG ASVILAELAM 

       310        320        330        340        350        360 
EAGLPDGVLN IVHGTNDTVN AICDDEDIRA VSFVGSNTAG MHIYARAAAK GKRIQSNMGA 

       370        380        390        400        410        420 
KNHGLVLPDA NIDATLNALL AAGFGAAGQR CMALSTVVFV GDAKSWEDKL VERAKALKVT 

       430        440        450        460        470        480 
CGSEPDADLG PVISKQAKER ICRLIQSGVD DGAKLLLDGR DIVVPGYEKG NFIGPTILSG 

       490        500        510        520        530        540 
VTPDMECYKE EIFGPVLVCM QANSFDEAIS IINKNKYGNG AAIFTSSGAA ARKFQMDIEA 

       550        560        570        580        590        600 
GQIGINVPIP VPLPFFSFTG NKASFAGDLN FYGKAGVDFF TQIKTVTQQW KDIPTSVSLA 


MPTSQKQ

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 294-607. Strain: cv. Columbia.
[3]	"The ALDH gene superfamily of Arabidopsis." Kirch H.-H., Bartels D., Wei Y., Schnable P.S., Wood A.J. Trends Plant Sci. 9:371-377(2004) [PubMed: 15358267] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007197 Genomic DNA. Translation: AAD25855.1. AK230004 mRNA. Translation: BAF01828.1.
IPI	IPI00518167.
PIR	H84514.
RefSeq	NP_179032.1.
UniGene	At.43851 Rra.7414 Rsa.5843
3D structure databases
SMR	Q0WM29. Positions 112-591.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0WM29.
Proteomic databases
PRIDE	Q0WM29.
Genome annotation databases
GeneID	815903.
GenomeReviews	Gene locus AT2G14170 in contig CT485783_GR.
NMPDR	fig\|3702.1.peg.8445.
Organism-specific databases
GeneFarm	4385.
TAIR	At2g14170.
Phylogenomic databases
HOGENOM	HBG752218.
InParanoid	Q0WM29.
OMA	IISNVKP.
PhylomeDB	Q0WM29.
Enzyme and pathway databases
BRENDA	1.2.1.27. 302.
Gene expression databases
Genevestigator	Q0WM29.
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

MMSA_ARATH

Accession

Primary (citable) accession number: Q0WM29
Secondary accession number(s): Q9SI43

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	February 9, 2010
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents