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Q0WLB5 (CLAH2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Clathrin heavy chain 2
Gene names
Name:CHC2
Synonyms:CHC1
Ordered Locus Names:At3g08530
ORF Names:F17O14.1, T8G24.1
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1703 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles By similarity. Mediates endocytosis and is required for a correct polar distribution of PIN auxin transporters. Ref.5

Subunit structure

Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat By similarity. Interacts with CLC2 and TPLATE. Ref.6

Subcellular location

Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Membranecoated pit; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Cytoplasmic face of coated pits and vesicles By similarity.

Domain

The C-terminal third of the heavy chains forms the hub of the triskelion. This region contains the trimerization domain and the light-chain binding domain involved in the assembly of the clathrin lattice.

The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It consitutes a major protein-protein interaction node By similarity.

Disruption phenotype

Defective in bulk endocytosis as well as in internalization of prominent plasma membrane proteins. Defects in constitutive endocytic recycling of PIN auxin transporters and their polar distribution in embryos and roots leading to altered auxin distribution patterns and associated auxin transport-related phenotypes. Defective in embryonic and postembryonic development. Ref.5

Sequence similarities

Belongs to the clathrin heavy chain family.

Contains 7 CHCR (clathrin heavy-chain) repeats.

Caution

Was assigned to CHC1 in Ref.6.

Sequence caution

The sequence AAG50828.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAG51341.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAM19776.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TPLATEF4J8D32EBI-4412194,EBI-4412119

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.7
Chain2 – 17031702Clathrin heavy chain 2
PRO_0000413950

Regions

Repeat551 – 697147CHCR 1
Repeat700 – 842143CHCR 2
Repeat847 – 986140CHCR 3
Repeat993 – 1138146CHCR 4
Repeat1142 – 1283142CHCR 5
Repeat1288 – 1434147CHCR 6
Repeat1437 – 1580144CHCR 7
Region2 – 492491Globular terminal domain By similarity
Region25 – 6743WD40-like repeat 1
Region68 – 11346WD40-like repeat 2
Region114 – 15542WD40-like repeat 3
Region156 – 20550WD40-like repeat 4
Region206 – 27065WD40-like repeat 5
Region271 – 31444WD40-like repeat 6
Region315 – 34329WD40-like repeat 7
Region462 – 47817Binding site for the uncoating ATPase, involved in lattice disassembly By similarity
Region493 – 53644Flexible linker By similarity
Region537 – 17031167Heavy chain arm By similarity
Region537 – 648112Distal segment By similarity
Region653 – 17031051Proximal segment By similarity
Region1227 – 1536310Involved in binding clathrin light chain By similarity
Region1564 – 1703140Trimerization By similarity

Amino acid modifications

Modified residue21N-acetylalanine Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q0WLB5 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 6A91D665A841F504

FASTA1,703193,271
        10         20         30         40         50         60 
MAAANAPITM KEVLTLPSIG INQQFITFTN VTMESDKYIC VRETSPQNSV VIIDMNMPMQ 

        70         80         90        100        110        120 
PLRRPITADS ALMNPNSKIL ALKAQVPGTT QDHLQIFNIE AKAKLKSHQM PEQVVFWKWI 

       130        140        150        160        170        180 
TPKMLGLVTQ NSVYHWSIEG DSEPVKMFDR TANLANNQII NYKCSPNEKW LVLIGIAPGS 

       190        200        210        220        230        240 
PERQQLVKGN MQLFSVDQQR SQALEAHAAS FAQFKVPGNE NPSILISFAS KSFNAGQITS 

       250        260        270        280        290        300 
KLHVIELGAQ PGKPSFTKKQ ADLFFPPDFA DDFPVAMQVS HKFNLIYVIT KLGLLFVYDL 

       310        320        330        340        350        360 
ETASAIYRNR ISPDPIFLTS EASSVGGFYA INRRGQVLLA TVNEATIIPF ISGQLNNLEL 

       370        380        390        400        410        420 
AVNLAKRGNL PGAENLVVQR FQELFAQTKY KEAAELAAES PQGILRTPDT VAKFQSVPVQ 

       430        440        450        460        470        480 
AGQTPPLLQY FGTLLTRGKL NSYESLELSR LVVNQNKKNL LENWLAEDKL ECSEELGDLV 

       490        500        510        520        530        540 
KTVDNDLALK IYIKARATPK VVAAFAERRE FDKILIYSKQ VGYTPDYLFL LQTILRTDPQ 

       550        560        570        580        590        600 
GAVNFALMMS QMEGGSPVDY NTITDLFLQR NLIREATSFL LDVLKPNLPE HAFLQTKVLE 

       610        620        630        640        650        660 
INLVTFPNVA DAVLANGMFT HYDRPRIAQL CEKAGLYIQS LKHYSELPDI KRVIVNTHAI 

       670        680        690        700        710        720 
EPQALVEFFG TLSSEWAMEC MKDLLLVNLR GNLQIIVQAC KEYCEQLGVD ACIKLFEQFK 

       730        740        750        760        770        780 
SYEGLYFFLG SYLSMSEDPE IHFKYIEAAA KTGQIKEVER VTRESNFYDA EKTKNFLMEA 

       790        800        810        820        830        840 
KLPDARPLIN VCDRFSFVPD LTHYLYTNNM LRYIEGYVQK VNPGNAPLVV GQLLDDECPE 

       850        860        870        880        890        900 
DFIKGLILSV RSLLPVEPLV EECEKRNRLR LLTQFLEHLV SEGSQDVHVH NALGKIIIDS 

       910        920        930        940        950        960 
NNNPEHFLTT NPYYDSKVVG KYCEKRDPTL AVVAYRRGQC DEELINVTNK NSLFKLQARY 

       970        980        990       1000       1010       1020 
VVERMDGDLW DKVLDENNDY RRQLIDQVVS TALPESKSPE QVSAAVKAFM TADLPHELIE 

      1030       1040       1050       1060       1070       1080 
LLEKIVLQNS AFSGNFNLQN LLILTAIKAD PSRVMDYINR LDNFDGPAVG EVAVEAQLYE 

      1090       1100       1110       1120       1130       1140 
EAFAIFKKFN LNVQAVNVLL DNVRSIERAV EFAFRVEEDS VWSQVAKAQL REGLVSDAIE 

      1150       1160       1170       1180       1190       1200 
SFIRADDATH FLEVIRVSED TDVYDDLVKY LLMVRQKVKE PKVDSELIYA YAKIDRLGEI 

      1210       1220       1230       1240       1250       1260 
EEFILMPNVA NLQHVGDRLY DEALYEAAKI IYAFISNWGK LAVTLVKLQQ FQGAVDAARK 

      1270       1280       1290       1300       1310       1320 
ANSAKTWKEV CFACVDAEEF RLAQICGLNI IIQVDDLEEV SEYYQNRGCF NELISLMESG 

      1330       1340       1350       1360       1370       1380 
LGLERAHMGI FTELGVLYAR YRYEKLMEHI KLFSTRLNIP KLIRACDEQQ HWQELTYLYI 

      1390       1400       1410       1420       1430       1440 
QYDEFDNAAT TVMNHSPEAW EHMQFKDIVA KVANVELYYK AVHFYLQEHP DIINDLLNVL 

      1450       1460       1470       1480       1490       1500 
ALRLDHTRVV DIMRKAGHLR LIKPYMIAVQ SNNVSAVNEA LNEIYVEEED YDRLRESIDL 

      1510       1520       1530       1540       1550       1560 
HDSFDQIGLA QKIEKHELVE MRRVAAYIYK KAGRWKQSIA LSKKDNMYKD CMETASQSGE 

      1570       1580       1590       1600       1610       1620 
HELAEQLLVY FIEQGKKECF ATCLFVCYDL IRPDVALELA WINNMMDFAF PYLLQFIREY 

      1630       1640       1650       1660       1670       1680 
SGKVDELIKD KLEAQKEVKA KEQEEKDVIS QQNMYAQMLP LALPAPPMPG MGGGGGYGPP 

      1690       1700 
PQMGGMPGMP PMPPYGMPPM GGY 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 973-1703.
Strain: cv. Columbia.
[5]"Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis."
Kitakura S., Vanneste S., Robert S., Loefke C., Teichmann T., Tanaka H., Friml J.
Plant Cell 23:1920-1931(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]"Adaptin-like protein TPLATE and clathrin recruitment during plant somatic cytokinesis occurs via two distinct pathways."
Van Damme D., Gadeyne A., Vanstraelen M., Inze D., Van Montagu M.C., De Jaeger G., Russinova E., Geelen D.
Proc. Natl. Acad. Sci. U.S.A. 108:615-620(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CLC2 AND TPLATE.
[7]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC012562 Genomic DNA. Translation: AAG51341.1. Sequence problems.
AC074395 Genomic DNA. Translation: AAG50828.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE74644.1.
AK230290 mRNA. Translation: BAF02092.1.
AY094397 mRNA. Translation: AAM19776.1. Different initiation.
AY125528 mRNA. Translation: AAM78038.1.
RefSeqNP_187466.4. NM_111688.6.
UniGeneAt.17332.
At.26828.

3D structure databases

ProteinModelPortalQ0WLB5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid5336. 47 interactions.
DIPDIP-59590N.
IntActQ0WLB5. 50 interactions.
STRING3702.AT3G08530.1-P.

Proteomic databases

PaxDbQ0WLB5.
PRIDEQ0WLB5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G08530.1; AT3G08530.1; AT3G08530.
GeneID820001.
KEGGath:AT3G08530.

Organism-specific databases

TAIRAT3G08530.

Phylogenomic databases

eggNOGNOG314149.
HOGENOMHOG000188877.
InParanoidQ0WLB5.
KOK04646.
OMASNLEIYY.
PhylomeDBQ0WLB5.
ProtClustDBCLSN2690853.

Gene expression databases

GenevestigatorQ0WLB5.

Family and domain databases

Gene3D1.25.40.10. 3 hits.
2.130.10.110. 1 hit.
InterProIPR016024. ARM-type_fold.
IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016025. Clathrin_H-chain_link/propller.
IPR015348. Clathrin_H-chain_linker_core.
IPR001473. Clathrin_H-chain_propeller_N.
IPR022365. Clathrin_H-chain_propeller_rpt.
IPR016341. Clathrin_heavy_chain.
IPR011990. TPR-like_helical.
[Graphical view]
PfamPF00637. Clathrin. 7 hits.
PF09268. Clathrin-link. 1 hit.
PF01394. Clathrin_propel. 2 hits.
[Graphical view]
PIRSFPIRSF002290. Clathrin_H_chain. 1 hit.
SMARTSM00299. CLH. 7 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 5 hits.
SSF50989. SSF50989. 1 hit.
PROSITEPS50236. CHCR. 7 hits.
[Graphical view]
ProtoNetSearch...

Other

PROQ0WLB5.

Entry information

Entry nameCLAH2_ARATH
AccessionPrimary (citable) accession number: Q0WLB5
Secondary accession number(s): Q8L3R8, Q9C6U0, Q9CA00
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: September 5, 2006
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names