Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q0WLB5

- CLAH2_ARATH

UniProt

Q0WLB5 - CLAH2_ARATH

Protein

Clathrin heavy chain 2

Gene

CHC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Clathrin is the major protein of the polyhedral coat of coated pits and vesicles By similarity. Mediates endocytosis and is required for a correct polar distribution of PIN auxin transporters.By similarity1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. structural molecule activity Source: InterPro

    GO - Biological processi

    1. endocytosis Source: TAIR
    2. intracellular protein transport Source: InterPro

    Keywords - Biological processi

    Endocytosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Clathrin heavy chain 2
    Gene namesi
    Name:CHC2
    Synonyms:CHC1
    Ordered Locus Names:At3g08530
    ORF Names:F17O14.1, T8G24.1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G08530.

    Subcellular locationi

    Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Membranecoated pit By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: Cytoplasmic face of coated pits and vesicles.By similarity

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. clathrin coat of coated pit Source: InterPro
    3. clathrin coat of trans-Golgi network vesicle Source: InterPro
    4. cytosol Source: TAIR
    5. Golgi apparatus Source: TAIR
    6. membrane Source: TAIR
    7. plasma membrane Source: TAIR
    8. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Coated pit, Cytoplasmic vesicle, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Defective in bulk endocytosis as well as in internalization of prominent plasma membrane proteins. Defects in constitutive endocytic recycling of PIN auxin transporters and their polar distribution in embryos and roots leading to altered auxin distribution patterns and associated auxin transport-related phenotypes. Defective in embryonic and postembryonic development.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 17031702Clathrin heavy chain 2PRO_0000413950Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ0WLB5.
    PRIDEiQ0WLB5.

    Expressioni

    Gene expression databases

    GenevestigatoriQ0WLB5.

    Interactioni

    Subunit structurei

    Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat By similarity. Interacts with CLC2 and TPLATE.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TPLATEF4J8D32EBI-4412194,EBI-4412119

    Protein-protein interaction databases

    BioGridi5336. 47 interactions.
    DIPiDIP-59590N.
    IntActiQ0WLB5. 51 interactions.
    STRINGi3702.AT3G08530.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0WLB5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati551 – 697147CHCR 1Add
    BLAST
    Repeati700 – 842143CHCR 2Add
    BLAST
    Repeati847 – 986140CHCR 3Add
    BLAST
    Repeati993 – 1138146CHCR 4Add
    BLAST
    Repeati1142 – 1283142CHCR 5Add
    BLAST
    Repeati1288 – 1434147CHCR 6Add
    BLAST
    Repeati1437 – 1580144CHCR 7Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 492491Globular terminal domainBy similarityAdd
    BLAST
    Regioni25 – 6743WD40-like repeat 1Add
    BLAST
    Regioni68 – 11346WD40-like repeat 2Add
    BLAST
    Regioni114 – 15542WD40-like repeat 3Add
    BLAST
    Regioni156 – 20550WD40-like repeat 4Add
    BLAST
    Regioni206 – 27065WD40-like repeat 5Add
    BLAST
    Regioni271 – 31444WD40-like repeat 6Add
    BLAST
    Regioni315 – 34329WD40-like repeat 7Add
    BLAST
    Regioni462 – 47817Binding site for the uncoating ATPase, involved in lattice disassemblyBy similarityAdd
    BLAST
    Regioni493 – 53644Flexible linkerBy similarityAdd
    BLAST
    Regioni537 – 17031167Heavy chain armBy similarityAdd
    BLAST
    Regioni537 – 648112Distal segmentBy similarityAdd
    BLAST
    Regioni653 – 17031051Proximal segmentBy similarityAdd
    BLAST
    Regioni1227 – 1536310Involved in binding clathrin light chainBy similarityAdd
    BLAST
    Regioni1564 – 1703140TrimerizationBy similarityAdd
    BLAST

    Domaini

    The C-terminal third of the heavy chains forms the hub of the triskelion. This region contains the trimerization domain and the light-chain binding domain involved in the assembly of the clathrin lattice.
    The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It consitutes a major protein-protein interaction node By similarity.By similarity

    Sequence similaritiesi

    Belongs to the clathrin heavy chain family.Curated
    Contains 7 CHCR (clathrin heavy-chain) repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG314149.
    HOGENOMiHOG000188877.
    InParanoidiQ0WLB5.
    KOiK04646.
    OMAiWKWFSEK.
    PhylomeDBiQ0WLB5.

    Family and domain databases

    Gene3Di1.25.40.10. 3 hits.
    2.130.10.110. 1 hit.
    InterProiIPR016024. ARM-type_fold.
    IPR000547. Clathrin_H-chain/VPS_repeat.
    IPR016025. Clathrin_H-chain_link/propller.
    IPR015348. Clathrin_H-chain_linker_core.
    IPR001473. Clathrin_H-chain_propeller_N.
    IPR022365. Clathrin_H-chain_propeller_rpt.
    IPR016341. Clathrin_heavy_chain.
    IPR011990. TPR-like_helical.
    [Graphical view]
    PfamiPF00637. Clathrin. 7 hits.
    PF09268. Clathrin-link. 1 hit.
    PF01394. Clathrin_propel. 2 hits.
    [Graphical view]
    PIRSFiPIRSF002290. Clathrin_H_chain. 1 hit.
    SMARTiSM00299. CLH. 7 hits.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 5 hits.
    SSF50989. SSF50989. 1 hit.
    PROSITEiPS50236. CHCR. 7 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q0WLB5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAANAPITM KEVLTLPSIG INQQFITFTN VTMESDKYIC VRETSPQNSV     50
    VIIDMNMPMQ PLRRPITADS ALMNPNSKIL ALKAQVPGTT QDHLQIFNIE 100
    AKAKLKSHQM PEQVVFWKWI TPKMLGLVTQ NSVYHWSIEG DSEPVKMFDR 150
    TANLANNQII NYKCSPNEKW LVLIGIAPGS PERQQLVKGN MQLFSVDQQR 200
    SQALEAHAAS FAQFKVPGNE NPSILISFAS KSFNAGQITS KLHVIELGAQ 250
    PGKPSFTKKQ ADLFFPPDFA DDFPVAMQVS HKFNLIYVIT KLGLLFVYDL 300
    ETASAIYRNR ISPDPIFLTS EASSVGGFYA INRRGQVLLA TVNEATIIPF 350
    ISGQLNNLEL AVNLAKRGNL PGAENLVVQR FQELFAQTKY KEAAELAAES 400
    PQGILRTPDT VAKFQSVPVQ AGQTPPLLQY FGTLLTRGKL NSYESLELSR 450
    LVVNQNKKNL LENWLAEDKL ECSEELGDLV KTVDNDLALK IYIKARATPK 500
    VVAAFAERRE FDKILIYSKQ VGYTPDYLFL LQTILRTDPQ GAVNFALMMS 550
    QMEGGSPVDY NTITDLFLQR NLIREATSFL LDVLKPNLPE HAFLQTKVLE 600
    INLVTFPNVA DAVLANGMFT HYDRPRIAQL CEKAGLYIQS LKHYSELPDI 650
    KRVIVNTHAI EPQALVEFFG TLSSEWAMEC MKDLLLVNLR GNLQIIVQAC 700
    KEYCEQLGVD ACIKLFEQFK SYEGLYFFLG SYLSMSEDPE IHFKYIEAAA 750
    KTGQIKEVER VTRESNFYDA EKTKNFLMEA KLPDARPLIN VCDRFSFVPD 800
    LTHYLYTNNM LRYIEGYVQK VNPGNAPLVV GQLLDDECPE DFIKGLILSV 850
    RSLLPVEPLV EECEKRNRLR LLTQFLEHLV SEGSQDVHVH NALGKIIIDS 900
    NNNPEHFLTT NPYYDSKVVG KYCEKRDPTL AVVAYRRGQC DEELINVTNK 950
    NSLFKLQARY VVERMDGDLW DKVLDENNDY RRQLIDQVVS TALPESKSPE 1000
    QVSAAVKAFM TADLPHELIE LLEKIVLQNS AFSGNFNLQN LLILTAIKAD 1050
    PSRVMDYINR LDNFDGPAVG EVAVEAQLYE EAFAIFKKFN LNVQAVNVLL 1100
    DNVRSIERAV EFAFRVEEDS VWSQVAKAQL REGLVSDAIE SFIRADDATH 1150
    FLEVIRVSED TDVYDDLVKY LLMVRQKVKE PKVDSELIYA YAKIDRLGEI 1200
    EEFILMPNVA NLQHVGDRLY DEALYEAAKI IYAFISNWGK LAVTLVKLQQ 1250
    FQGAVDAARK ANSAKTWKEV CFACVDAEEF RLAQICGLNI IIQVDDLEEV 1300
    SEYYQNRGCF NELISLMESG LGLERAHMGI FTELGVLYAR YRYEKLMEHI 1350
    KLFSTRLNIP KLIRACDEQQ HWQELTYLYI QYDEFDNAAT TVMNHSPEAW 1400
    EHMQFKDIVA KVANVELYYK AVHFYLQEHP DIINDLLNVL ALRLDHTRVV 1450
    DIMRKAGHLR LIKPYMIAVQ SNNVSAVNEA LNEIYVEEED YDRLRESIDL 1500
    HDSFDQIGLA QKIEKHELVE MRRVAAYIYK KAGRWKQSIA LSKKDNMYKD 1550
    CMETASQSGE HELAEQLLVY FIEQGKKECF ATCLFVCYDL IRPDVALELA 1600
    WINNMMDFAF PYLLQFIREY SGKVDELIKD KLEAQKEVKA KEQEEKDVIS 1650
    QQNMYAQMLP LALPAPPMPG MGGGGGYGPP PQMGGMPGMP PMPPYGMPPM 1700
    GGY 1703
    Length:1,703
    Mass (Da):193,271
    Last modified:September 5, 2006 - v1
    Checksum:i6A91D665A841F504
    GO

    Sequence cautioni

    The sequence AAM19776.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAG50828.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence AAG51341.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC012562 Genomic DNA. Translation: AAG51341.1. Sequence problems.
    AC074395 Genomic DNA. Translation: AAG50828.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE74644.1.
    AK230290 mRNA. Translation: BAF02092.1.
    AY094397 mRNA. Translation: AAM19776.1. Different initiation.
    AY125528 mRNA. Translation: AAM78038.1.
    RefSeqiNP_187466.4. NM_111688.6.
    UniGeneiAt.17332.
    At.26828.

    Genome annotation databases

    EnsemblPlantsiAT3G08530.1; AT3G08530.1; AT3G08530.
    GeneIDi820001.
    KEGGiath:AT3G08530.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC012562 Genomic DNA. Translation: AAG51341.1 . Sequence problems.
    AC074395 Genomic DNA. Translation: AAG50828.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE74644.1 .
    AK230290 mRNA. Translation: BAF02092.1 .
    AY094397 mRNA. Translation: AAM19776.1 . Different initiation.
    AY125528 mRNA. Translation: AAM78038.1 .
    RefSeqi NP_187466.4. NM_111688.6.
    UniGenei At.17332.
    At.26828.

    3D structure databases

    ProteinModelPortali Q0WLB5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 5336. 47 interactions.
    DIPi DIP-59590N.
    IntActi Q0WLB5. 51 interactions.
    STRINGi 3702.AT3G08530.1-P.

    Proteomic databases

    PaxDbi Q0WLB5.
    PRIDEi Q0WLB5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G08530.1 ; AT3G08530.1 ; AT3G08530 .
    GeneIDi 820001.
    KEGGi ath:AT3G08530.

    Organism-specific databases

    TAIRi AT3G08530.

    Phylogenomic databases

    eggNOGi NOG314149.
    HOGENOMi HOG000188877.
    InParanoidi Q0WLB5.
    KOi K04646.
    OMAi WKWFSEK.
    PhylomeDBi Q0WLB5.

    Miscellaneous databases

    PROi Q0WLB5.

    Gene expression databases

    Genevestigatori Q0WLB5.

    Family and domain databases

    Gene3Di 1.25.40.10. 3 hits.
    2.130.10.110. 1 hit.
    InterProi IPR016024. ARM-type_fold.
    IPR000547. Clathrin_H-chain/VPS_repeat.
    IPR016025. Clathrin_H-chain_link/propller.
    IPR015348. Clathrin_H-chain_linker_core.
    IPR001473. Clathrin_H-chain_propeller_N.
    IPR022365. Clathrin_H-chain_propeller_rpt.
    IPR016341. Clathrin_heavy_chain.
    IPR011990. TPR-like_helical.
    [Graphical view ]
    Pfami PF00637. Clathrin. 7 hits.
    PF09268. Clathrin-link. 1 hit.
    PF01394. Clathrin_propel. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF002290. Clathrin_H_chain. 1 hit.
    SMARTi SM00299. CLH. 7 hits.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 5 hits.
    SSF50989. SSF50989. 1 hit.
    PROSITEi PS50236. CHCR. 7 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 973-1703.
      Strain: cv. Columbia.
    5. "Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis."
      Kitakura S., Vanneste S., Robert S., Loefke C., Teichmann T., Tanaka H., Friml J.
      Plant Cell 23:1920-1931(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "Adaptin-like protein TPLATE and clathrin recruitment during plant somatic cytokinesis occurs via two distinct pathways."
      Van Damme D., Gadeyne A., Vanstraelen M., Inze D., Van Montagu M.C., De Jaeger G., Russinova E., Geelen D.
      Proc. Natl. Acad. Sci. U.S.A. 108:615-620(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CLC2 AND TPLATE.
    7. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCLAH2_ARATH
    AccessioniPrimary (citable) accession number: Q0WLB5
    Secondary accession number(s): Q8L3R8, Q9C6U0, Q9CA00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2011
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Caution

    Was assigned to CHC1 in PubMed:21187379.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3