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Protein

Xyloglucan endotransglucosylase/hydrolase

Gene

At4g30270

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.UniRule annotation

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseUniRule annotation, Hydrolase, TransferaseUniRule annotation
Biological processCell wall biogenesis/degradationUniRule annotation

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolaseUniRule annotation (EC:2.4.1.207UniRule annotation)
Gene namesi
Ordered Locus Names:At4g30270Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Subcellular locationi

  • Secretedcell wall UniRule annotation
  • Secretedextracellular spaceapoplast UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

ApoplastUniRule annotation, Cell wallUniRule annotation, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21UniRule annotationAdd BLAST21
ChainiPRO_501039640222 – 269Xyloglucan endotransglucosylase/hydrolaseUniRule annotationAdd BLAST248

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.UniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G30270.1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 212GH16 (glycosyl hydrolase family 16)InterPro annotationAdd BLAST212

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Phylogenomic databases

eggNOGiENOG410JBCJ. Eukaryota.
COG2273. LUCA.
KOiK08235.
OMAiSANACAW.
OrthoDBiEOG09360GR5.

Family and domain databases

InterProiView protein in InterPro
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
IPR010713. XET_C.
IPR016455. XTH.
PfamiView protein in Pfam
PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0WLB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPFKIFFFT TLLVAAFSVS AADFNTDVNV AWGNGRGKIL NNGQLLTLSL
60 70 80 90 100
DKSSGSGFQS KTEYLFGKID MQIKLVPGNS AGTVTTFYLK SEGSTWDEID
110 120 130 140 150
FEFLGNMSGD PYTLHTNVYT QGKGDKEQQF HLWFDPTANF HTYSILWNPQ
160 170 180 190 200
RIILTVDDTP IREFKNYESL GVLFPKNKPM RMYASLWNAD DWATRGGLVK
210 220 230 240 250
TDWSKAPFMA SYRNIKIDSK PNSNWYTQEM DSTSQARLKW VQKNYMIYNY
260
CTDHRRFPQG APKECTTSS
Length:269
Mass (Da):30,756
Last modified:September 5, 2006 - v1
Checksum:i648F042BC7ADED86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK229363 mRNA. Translation: BAF01226.1.
AK230296 mRNA. Translation: BAF02097.1.
RefSeqiNP_194756.1. NM_119173.4.
UniGeneiAt.20967.
At.26243.
At.27681.
At.47568.
At.75103.

Genome annotation databases

EnsemblPlantsiAT4G30270.1; AT4G30270.1; AT4G30270.
GeneIDi829150.
GrameneiAT4G30270.1; AT4G30270.1; AT4G30270.
KEGGiath:AT4G30270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK229363 mRNA. Translation: BAF01226.1.
AK230296 mRNA. Translation: BAF02097.1.
RefSeqiNP_194756.1. NM_119173.4.
UniGeneiAt.20967.
At.26243.
At.27681.
At.47568.
At.75103.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G30270.1.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G30270.1; AT4G30270.1; AT4G30270.
GeneIDi829150.
GrameneiAT4G30270.1; AT4G30270.1; AT4G30270.
KEGGiath:AT4G30270.

Phylogenomic databases

eggNOGiENOG410JBCJ. Eukaryota.
COG2273. LUCA.
KOiK08235.
OMAiSANACAW.
OrthoDBiEOG09360GR5.

Family and domain databases

InterProiView protein in InterPro
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
IPR010713. XET_C.
IPR016455. XTH.
PfamiView protein in Pfam
PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQ0WLB0_ARATH
AccessioniPrimary (citable) accession number: Q0WLB0
Entry historyiIntegrated into UniProtKB/TrEMBL: September 5, 2006
Last sequence update: September 5, 2006
Last modified: June 7, 2017
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.