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Protein

Bifunctional aspartokinase/homoserine dehydrogenase

Gene

thrA

Organism
Yersinia pestis
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-aspartate = ADP + 4-phospho-L-aspartate.UniRule annotation
L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (AU253_09460), Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Aspartokinase (lysC2), Aspartokinase (apk), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Bifunctional aspartokinase/homoserine dehydrogenase (thrA)
  2. Aspartate-semialdehyde dehydrogenase (asd), Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA), 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB), 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 and 3 of the subpathway that synthesizes L-homoserine from L-aspartate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Aspartokinase (AU253_09460), Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Aspartokinase (lysC2), Aspartokinase (apk), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Bifunctional aspartokinase/homoserine dehydrogenase (thrA)
  2. Aspartate-semialdehyde dehydrogenase (asd), Aspartate-semialdehyde dehydrogenase (asd)
  3. Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Bifunctional aspartokinase/homoserine dehydrogenase (thrA)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homoserine from L-aspartate, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 1 and 3 of the subpathway that synthesizes L-threonine from L-aspartate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (AU253_09460), Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Aspartokinase (lysC2), Aspartokinase (apk), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Bifunctional aspartokinase/homoserine dehydrogenase (thrA)
  2. Aspartate-semialdehyde dehydrogenase (asd), Aspartate-semialdehyde dehydrogenase (asd)
  3. Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Bifunctional aspartokinase/homoserine dehydrogenase (thrA)
  4. Homoserine kinase (thrB), Homoserine kinase (thrB)
  5. no protein annotated in this organism
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationImported, OxidoreductaseUniRule annotation, Transferase
Biological processAmino-acid biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, NADPUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00034; UER00015
UPA00050; UER00063
UPA00050; UER00461
UPA00051; UER00462
UPA00051; UER00465

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional aspartokinase/homoserine dehydrogenaseUniRule annotation
Including the following 2 domains:
AspartokinaseUniRule annotation (EC:2.7.2.4UniRule annotation)
Homoserine dehydrogenaseUniRule annotation (EC:1.1.1.3UniRule annotation)
Gene namesi
Name:thrAImported
Ordered Locus Names:YPO0459Imported
OrganismiYersinia pestisImported
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Proteomic databases

PaxDbiQ0WJK7

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi187410.y3718

Structurei

3D structure databases

ProteinModelPortaliQ0WJK7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini320 – 394ACTInterPro annotationAdd BLAST75
Domaini401 – 478ACTInterPro annotationAdd BLAST78

Sequence similaritiesi

In the C-terminal section; belongs to the homoserine dehydrogenase family.UniRule annotation
In the N-terminal section; belongs to the aspartokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CFH Bacteria
COG0460 LUCA
COG0527 LUCA
HOGENOMiHOG000271593
KOiK12524
OMAiCNKIACS

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR002912 ACT_dom
IPR001048 Asp/Glu/Uridylate_kinase
IPR005106 Asp/hSer_DH_NAD-bd
IPR001341 Asp_kinase
IPR018042 Aspartate_kinase_CS
IPR011147 Bifunc_aspartokin/hSer_DH
IPR027795 CASTOR_ACT_dom
IPR001342 HDH_cat
IPR019811 HDH_CS
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01842 ACT, 1 hit
PF13840 ACT_7, 1 hit
PF00742 Homoserine_dh, 1 hit
PF03447 NAD_binding_3, 1 hit
PIRSFiPIRSF000727 ThrA, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR00657 asp_kinases, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS00324 ASPARTOKINASE, 1 hit
PS01042 HOMOSER_DHGENASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q0WJK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLKFGGTS VANAERFMRV ADIIESNARQ GQVATVLSAP AKITNHLVAM
60 70 80 90 100
IDKMVAGQDI SPNISDAERI FAELLRGLAD TQPGFDYDRL KALVGHEFAQ
110 120 130 140 150
LKHLLHGISL LGQCPDSINA SIICRGEKLS IAIMEALFQA KGYHVTVINP
160 170 180 190 200
VEKLLAQGHY LESTVDITES TRRIGASGIP SDHIILMAGF TAGNDKGELV
210 220 230 240 250
VLGRNGSDYS AAVLAACLRA DCCEIWTDVD GVYTCDPRTV PDARLLKSMS
260 270 280 290 300
YQEAMELSYF GAKVLHPRTI APIARFQIPC LIKNTSNPQA PGTLIGGESI
310 320 330 340 350
DEDSPVKGIT NLNNMAMINV SGPGMKGMVG MAARVFAVMS RSGISVVLIT
360 370 380 390 400
QSSSEYSISF CVPQGELLRA RRALEDEFYL ELKDGVLDPL DVMEHLAIIS
410 420 430 440 450
VVGDGMRTLR GISARFFSAL ARANINIIAI AQGSSERSIS VVVNNDAVTT
460 470 480 490 500
GVRVCHQMLF NTDQVIEVFV IGVGGVGGAL IEQIYRQQPW LKQRHIDLRV
510 520 530 540 550
CGIANSKAML TNVHGIALDN WRQELAEVQE PFNLSRLIRL VKEYHLLNPV
560 570 580 590 600
IVDCTSSQAV ADQYADFLTD GFHVVTPNKK ANTSSMNYYR QMRAAATKSC
610 620 630 640 650
RKFLYDTNVG AGLPVIENLQ NLLNAGDELM RFTGILSGSL SFIFGKLDEG
660 670 680 690 700
MSLSEATRQA KALGYTEPDP RDDLSGMDVA RKLLILAREA GYKLELADIE
710 720 730 740 750
VESVLPASFD ASGDVDTFLA RLPSLDAEFT RLVANAAEQG KVLRYVGVIE
760 770 780 790 800
DGRCKVRMEA VDGNDPLYKV KNGENALAFY TRYYQPIPLV LRGYGAGNDV
810
TAAGVFADLL RTLSWKLGV
Length:819
Mass (Da):89,110
Last modified:September 5, 2006 - v1
Checksum:iE8249C805C945285
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL19138.1
PIRiAH0056
RefSeqiWP_002209237.1, NZ_PDBR01000043.1
YP_002345531.1, NC_003143.1

Genome annotation databases

GeneIDi1173303
KEGGiype:YPO0459
ypj:CH55_2348
ypl:CH46_454
ypv:BZ15_3111
ypw:CH59_1401
PATRICifig|214092.21.peg.705

Similar proteinsi

Entry informationi

Entry nameiQ0WJK7_YERPE
AccessioniPrimary (citable) accession number: Q0WJK7
Entry historyiIntegrated into UniProtKB/TrEMBL: September 5, 2006
Last sequence update: September 5, 2006
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health