Reviewed, UniProtKB/Swiss-Prot Q0WGE8 (NAGK_YERPE)

Names and origin

Protein names

Recommended name:
    N-acetyl-D-glucosamine kinase
    EC=2.7.1.59
Alternative name(s):
    GlcNAc kinase

Gene names

Name:	nagK
Ordered Locus Names:	YPO1629, y1788, YP_1758

Organism

Yersinia pestis [Complete proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P By similarity.
Catalytic activity	ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate. HAMAP MF_01271
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP MF_01271
Miscellaneous	Might be inactive, since the sequence is shorter than in other family members, due to a probable natural frameshift in the C-terminus. HAMAP MF_01271
Sequence similarities	Belongs to the ROK (nagC/xylR) family. NagK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: HAMAP peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP N-acetylglucosamine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	N-acetyl-D-glucosamine kinase HAMAP MF_01271		PRO_0000270125
Regions
	Nucleotide binding	4 – 11	8	ATP Potential
	Nucleotide binding	133 – 140	8	ATP Potential
Sites
	Metal binding	157	1	Zinc By similarity
	Metal binding	177	1	Zinc By similarity
	Metal binding	179	1	Zinc By similarity
	Metal binding	184	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0WGE8-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 0BCE117500ABD548	FASTA	256	27,879
	10 20 30 40 50 60 MYYGFDMGGT KIELGVFDEN LQRIWHKRVP TPREDYPQLL QILRDLTEEA DTYCGVQGSV 70 80 90 100 110 120 GIGIPGLPNA DDGTVFTANV PSAMGQPLQA DLSRLIQREV RIDNDANCFA LSEAWDPEFR 130 140 150 160 170 180 TYPTVLGLIL GTGVGGGLIV NGSIVSGRNH ITGEFGHFRL PVDALDILGA DIPRVPCGCG 190 200 210 220 230 240 HRGCIENYIS GRGFEWMYSH FYQHTLPATD IIAHYAAGEP KAVAHVERFM DVLAVCLGNL 250 LTMLGSPFGR GGWGVV

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL590842 Genomic DNA. Translation: CAL20274.1. AE009952 Genomic DNA. Translation: AAM85356.1. Different initiation. AE017042 Genomic DNA. Translation: AAS61985.1. Different initiation.
PIR	AH0198.
RefSeq	NP_669105.2. NP_993108.2. YP_002346640.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1146735. 1174468. 2765632.
GenomeReviews	Gene locus y1788 in contig AE009952_GR. Gene locus YP_1758 in contig AE017042_GR. Gene locus YPO1629 in contig AL590842_GR.
KEGG	ype:YPO1629. ypk:y1788. ypm:YP_1758.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0WGE8.
OMA	Q0WGE8. CAETYVS.
Enzyme and pathway databases
BioCyc	YPES187410:Y1788-MON. YPES229193:YP1758-MON.
BRENDA	2.7.1.59. 142588.
Family and domain databases
HAMAP	MF_01271. Divergent sequence. [Tree]
InterPro	IPR000600. ROK. [Graphical view]
Pfam	PF00480. ROK. 1 hit. [Graphical view]
PROSITE	PS01125. ROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGK_YERPE

Accession

Primary (citable) accession number: Q0WGE8
Secondary accession number(s): Q74UG3, Q8D0Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	September 5, 2006
Last modified:	May 5, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)