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Q0WD32

- IMDH_YERPE

UniProt

Q0WD32 - IMDH_YERPE

Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Yersinia pestis
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

    Catalytic activityi

    Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

    Cofactori

    Potassium.UniRule annotation

    Enzyme regulationi

    Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei248 – 2481NADUniRule annotation
    Metal bindingi300 – 3001Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi302 – 3021Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei303 – 3031IMPUniRule annotation
    Active sitei305 – 3051Thioimidate intermediateUniRule annotation
    Metal bindingi305 – 3051Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei415 – 4151IMPUniRule annotation
    Metal bindingi469 – 4691Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi470 – 4701Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi471 – 4711Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi248 – 2503NADUniRule annotation
    Nucleotide bindingi298 – 3003NADUniRule annotation

    GO - Molecular functioni

    1. adenyl nucleotide binding Source: InterPro
    2. IMP dehydrogenase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. GMP biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    GMP biosynthesis, Purine biosynthesis

    Keywords - Ligandi

    Metal-binding, NAD, Potassium

    Enzyme and pathway databases

    BioCyciYPES214092:GKDD-2833-MONOMER.
    UniPathwayiUPA00601; UER00295.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
    Short name:
    IMP dehydrogenaseUniRule annotation
    Short name:
    IMPDUniRule annotation
    Short name:
    IMPDHUniRule annotation
    Gene namesi
    Name:guaBUniRule annotation
    Ordered Locus Names:YPO2871, y1362, YP_2737
    OrganismiYersinia pestis
    Taxonomic identifieri632 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000000815: Chromosome, UP000001019: Chromosome, UP000002490: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 487487Inosine-5'-monophosphate dehydrogenasePRO_0000415691Add
    BLAST

    Proteomic databases

    PRIDEiQ0WD32.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi214092.YPO2871.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0WD32.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini93 – 15260CBS 1UniRule annotationAdd
    BLAST
    Domaini153 – 21462CBS 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni338 – 3403IMP bindingUniRule annotation
    Regioni361 – 3622IMP bindingUniRule annotation
    Regioni385 – 3895IMP bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the IMPDH/GMPR family.UniRule annotation
    Contains 2 CBS domains.UniRule annotation

    Keywords - Domaini

    CBS domain, Repeat

    Phylogenomic databases

    HOGENOMiHOG000165755.
    KOiK00088.
    OMAiHGHSKNI.
    OrthoDBiEOG6GTZPV.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01964. IMPDH.
    InterProiIPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view]
    PfamiPF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000130. IMPDH. 1 hit.
    SMARTiSM00116. CBS. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
    PROSITEiPS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0WD32-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRIAKEALT FDDVLLVPAH STVLPNTAEL GTQLTATIRL NIPMLSAAMD    50
    TVTESRLAIA LAQEGGLGFI HKNMSIERQA EEVSRVKKHE SGVVTEPQTV 100
    TPTTTLRQVK ELTARNGFAG YPVVTEDYEL VGIITGRDVR FVTDLDQPVT 150
    AVMTPKERLV TVKEGETREV VLQKMHEKRV EKVLVVDDSF HLRGMITVKD 200
    FQKAERKPNA CKDEHGRLRV GAAVGAGAGN EERIDALVAA GVDVLLIDSS 250
    HGHSEGVLQR IRETRAKYPN LQIVGGNVAT GAGAKALADA GVSAVKVGIG 300
    PGSICTTRIV TGVGVPQITA IADAVEALEG TGIPVIADGG IRFSGDIAKA 350
    IAAGASCVMV GSMLAGTEES PGEIELYQGR SFKSYRGMGS LGAMSKGSSD 400
    RYFQTDNAAD KLVPEGIEGR VAYKGLLKEI VHQQMGGLRS CMGLTGCGTI 450
    NELRTKAEFV RISGAGIQES HVHDVTITKE SPNYRMM 487
    Length:487
    Mass (Da):51,824
    Last modified:September 5, 2006 - v1
    Checksum:i97A043EB8F1BA09A
    GO

    Sequence cautioni

    The sequence AAM84935.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence AAS62925.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590842 Genomic DNA. Translation: CAL21482.1.
    AE009952 Genomic DNA. Translation: AAM84935.1. Different initiation.
    AE017042 Genomic DNA. Translation: AAS62925.1. Different initiation.
    PIRiAG0349.
    RefSeqiNP_668684.1. NC_004088.1.
    NP_994048.1. NC_005810.1.
    YP_002347806.1. NC_003143.1.

    Genome annotation databases

    EnsemblBacteriaiAAM84935; AAM84935; y1362.
    AAS62925; AAS62925; YP_2737.
    GeneIDi1146309.
    1175695.
    2765560.
    KEGGiype:YPO2871.
    ypk:y1362.
    ypm:YP_2737.
    PATRICi18595578. VBIYerPes7843_3491.
    18624444. VBIYerPes99487_3147.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590842 Genomic DNA. Translation: CAL21482.1 .
    AE009952 Genomic DNA. Translation: AAM84935.1 . Different initiation.
    AE017042 Genomic DNA. Translation: AAS62925.1 . Different initiation.
    PIRi AG0349.
    RefSeqi NP_668684.1. NC_004088.1.
    NP_994048.1. NC_005810.1.
    YP_002347806.1. NC_003143.1.

    3D structure databases

    ProteinModelPortali Q0WD32.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 214092.YPO2871.

    Proteomic databases

    PRIDEi Q0WD32.

    Protocols and materials databases

    DNASUi 1146309.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM84935 ; AAM84935 ; y1362 .
    AAS62925 ; AAS62925 ; YP_2737 .
    GeneIDi 1146309.
    1175695.
    2765560.
    KEGGi ype:YPO2871.
    ypk:y1362.
    ypm:YP_2737.
    PATRICi 18595578. VBIYerPes7843_3491.
    18624444. VBIYerPes99487_3147.

    Phylogenomic databases

    HOGENOMi HOG000165755.
    KOi K00088.
    OMAi HGHSKNI.
    OrthoDBi EOG6GTZPV.

    Enzyme and pathway databases

    UniPathwayi UPA00601 ; UER00295 .
    BioCyci YPES214092:GKDD-2833-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01964. IMPDH.
    InterProi IPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view ]
    Pfami PF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000130. IMPDH. 1 hit.
    SMARTi SM00116. CBS. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
    PROSITEi PS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CO-92 / Biovar Orientalis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KIM10+ / Biovar Mediaevalis.
    3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
      Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
      , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
      DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 91001 / Biovar Mediaevalis.

    Entry informationi

    Entry nameiIMDH_YERPE
    AccessioniPrimary (citable) accession number: Q0WD32
    Secondary accession number(s): Q74S88, Q8D0Z1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2012
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3