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Q0WD32

- IMDH_YERPE

UniProt

Q0WD32 - IMDH_YERPE

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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Yersinia pestis
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

Potassium.UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei248 – 2481NADUniRule annotation
Metal bindingi300 – 3001Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi302 – 3021Potassium; via carbonyl oxygenUniRule annotation
Binding sitei303 – 3031IMPUniRule annotation
Active sitei305 – 3051Thioimidate intermediateUniRule annotation
Metal bindingi305 – 3051Potassium; via carbonyl oxygenUniRule annotation
Binding sitei415 – 4151IMPUniRule annotation
Metal bindingi469 – 4691Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
Metal bindingi470 – 4701Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
Metal bindingi471 – 4711Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi248 – 2503NADUniRule annotation
Nucleotide bindingi298 – 3003NADUniRule annotation

GO - Molecular functioni

  1. adenyl nucleotide binding Source: InterPro
  2. IMP dehydrogenase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. GMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Potassium

Enzyme and pathway databases

BioCyciYPES214092:GKDD-2833-MONOMER.
UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenaseUniRule annotation
Short name:
IMPDUniRule annotation
Short name:
IMPDHUniRule annotation
Gene namesi
Name:guaBUniRule annotation
Ordered Locus Names:YPO2871, y1362, YP_2737
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000815: Chromosome, UP000001019: Chromosome, UP000002490: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487Inosine-5'-monophosphate dehydrogenasePRO_0000415691Add
BLAST

Proteomic databases

PRIDEiQ0WD32.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi214092.YPO2871.

Structurei

3D structure databases

ProteinModelPortaliQ0WD32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini93 – 15260CBS 1UniRule annotationAdd
BLAST
Domaini153 – 21462CBS 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni338 – 3403IMP bindingUniRule annotation
Regioni361 – 3622IMP bindingUniRule annotation
Regioni385 – 3895IMP bindingUniRule annotation

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation
Contains 2 CBS domains.UniRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

HOGENOMiHOG000165755.
KOiK00088.
OMAiHGHSKNI.
OrthoDBiEOG6GTZPV.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0WD32-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRIAKEALT FDDVLLVPAH STVLPNTAEL GTQLTATIRL NIPMLSAAMD
60 70 80 90 100
TVTESRLAIA LAQEGGLGFI HKNMSIERQA EEVSRVKKHE SGVVTEPQTV
110 120 130 140 150
TPTTTLRQVK ELTARNGFAG YPVVTEDYEL VGIITGRDVR FVTDLDQPVT
160 170 180 190 200
AVMTPKERLV TVKEGETREV VLQKMHEKRV EKVLVVDDSF HLRGMITVKD
210 220 230 240 250
FQKAERKPNA CKDEHGRLRV GAAVGAGAGN EERIDALVAA GVDVLLIDSS
260 270 280 290 300
HGHSEGVLQR IRETRAKYPN LQIVGGNVAT GAGAKALADA GVSAVKVGIG
310 320 330 340 350
PGSICTTRIV TGVGVPQITA IADAVEALEG TGIPVIADGG IRFSGDIAKA
360 370 380 390 400
IAAGASCVMV GSMLAGTEES PGEIELYQGR SFKSYRGMGS LGAMSKGSSD
410 420 430 440 450
RYFQTDNAAD KLVPEGIEGR VAYKGLLKEI VHQQMGGLRS CMGLTGCGTI
460 470 480
NELRTKAEFV RISGAGIQES HVHDVTITKE SPNYRMM
Length:487
Mass (Da):51,824
Last modified:September 5, 2006 - v1
Checksum:i97A043EB8F1BA09A
GO

Sequence cautioni

The sequence AAM84935.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAS62925.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590842 Genomic DNA. Translation: CAL21482.1.
AE009952 Genomic DNA. Translation: AAM84935.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS62925.1. Different initiation.
PIRiAG0349.
RefSeqiNP_668684.1. NC_004088.1.
NP_994048.1. NC_005810.1.
YP_002347806.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM84935; AAM84935; y1362.
AAS62925; AAS62925; YP_2737.
GeneIDi1146309.
1175695.
2765560.
KEGGiype:YPO2871.
ypk:y1362.
ypm:YP_2737.
PATRICi18595578. VBIYerPes7843_3491.
18624444. VBIYerPes99487_3147.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590842 Genomic DNA. Translation: CAL21482.1 .
AE009952 Genomic DNA. Translation: AAM84935.1 . Different initiation.
AE017042 Genomic DNA. Translation: AAS62925.1 . Different initiation.
PIRi AG0349.
RefSeqi NP_668684.1. NC_004088.1.
NP_994048.1. NC_005810.1.
YP_002347806.1. NC_003143.1.

3D structure databases

ProteinModelPortali Q0WD32.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 214092.YPO2871.

Proteomic databases

PRIDEi Q0WD32.

Protocols and materials databases

DNASUi 1146309.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM84935 ; AAM84935 ; y1362 .
AAS62925 ; AAS62925 ; YP_2737 .
GeneIDi 1146309.
1175695.
2765560.
KEGGi ype:YPO2871.
ypk:y1362.
ypm:YP_2737.
PATRICi 18595578. VBIYerPes7843_3491.
18624444. VBIYerPes99487_3147.

Phylogenomic databases

HOGENOMi HOG000165755.
KOi K00088.
OMAi HGHSKNI.
OrthoDBi EOG6GTZPV.

Enzyme and pathway databases

UniPathwayi UPA00601 ; UER00295 .
BioCyci YPES214092:GKDD-2833-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01964. IMPDH.
InterProi IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view ]
Pfami PF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view ]
PIRSFi PIRSF000130. IMPDH. 1 hit.
SMARTi SM00116. CBS. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
PROSITEi PS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIM10+ / Biovar Mediaevalis.
  3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.

Entry informationi

Entry nameiIMDH_YERPE
AccessioniPrimary (citable) accession number: Q0WD32
Secondary accession number(s): Q74S88, Q8D0Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: September 5, 2006
Last modified: October 1, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3