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Q0WBF9

- PNP_YERPE

UniProt

Q0WBF9 - PNP_YERPE

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Yersinia pestis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi486 – 4861MagnesiumUniRule annotation
Metal bindingi492 – 4921MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciYPES214092:GKDD-3444-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:YPO3490, y0694, YP_0593
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000815: Chromosome, UP000001019: Chromosome, UP000002490: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 705705Polyribonucleotide nucleotidyltransferasePRO_0000329947Add
BLAST

Proteomic databases

PRIDEiQ0WBF9.

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

IntActiQ0WBF9. 5 interactions.
STRINGi214092.YPO3490.

Structurei

3D structure databases

ProteinModelPortaliQ0WBF9.
SMRiQ0WBF9. Positions 617-692.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini553 – 61260KHUniRule annotationAdd
BLAST
Domaini622 – 69069S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0WBF9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLTPIIRKFQ YGQHTVTIET GMMARQATAA VMVSMDDTAV FVTVVGQKKA
60 70 80 90 100
KPGQSFFPLT VNYQERTYAA GRIPGSFFRR EGRPSEGETL TSRLIDRPIR
110 120 130 140 150
PLFPDSFLNE VQVIATVVSV NPQINPDIVA LIGASAALSL SGIPFNGPIG
160 170 180 190 200
AARVGFINDQ YVLNPTTDEL KESRLDLVVA GTAGAVLMVE SEADILSEEQ
210 220 230 240 250
MLGAVVFGHE QQQVVIENIN ALVAEAGKPK WDWQAEPVNE ALHARVAELA
260 270 280 290 300
EARLGDAYRI TEKQERYTQV DAIKADVTEA LLAQDDTLDA AEIQDILASV
310 320 330 340 350
EKNVVRSRVL RGEPRIDGRE KDMIRGLDVR TGILPRTHGS ALFTRGETQA
360 370 380 390 400
LVTATLGTAR DAQNIDELMG ERTDSFLLHY NFPPYCVGET GMVGSPKRRE
410 420 430 440 450
IGHGRLAKRG VLAVMPSASE FPYTIRVVSE ITESNGSSSM ASVCGASLAL
460 470 480 490 500
MDAGVPIKAA VAGIAMGLVK EGDNFVVLSD ILGDEDHLGD MDFKVAGSRD
510 520 530 540 550
GVTALQMDIK IEGITREIMQ VALNQAKGAR LHILGVMEQA ISTPRGDISE
560 570 580 590 600
FAPRIYTMKI NPEKIKDVIG KGGSVIRALT DETGTTIEIE DDGTIKIAAT
610 620 630 640 650
DGDKAKHAIR RIEEITAEIE VGRIYAGKVT RIVDFGAFVA IGGGKEGLVH
660 670 680 690 700
ISQIADKRVE KVTDYLQMGQ DVPVKVMEVD RQGRIRLSIK EATTPDAEAP

EAAAE
Length:705
Mass (Da):76,196
Last modified:September 5, 2006 - v1
Checksum:iE59E8F1724574722
GO

Sequence cautioni

The sequence AAM84282.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAS60863.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590842 Genomic DNA. Translation: CAL22078.1.
AE009952 Genomic DNA. Translation: AAM84282.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS60863.1. Different initiation.
PIRiAC0424.
RefSeqiNP_668031.1. NC_004088.1.
NP_991986.1. NC_005810.1.
YP_002348379.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM84282; AAM84282; y0694.
AAS60863; AAS60863; YP_0593.
GeneIDi1145641.
1176307.
2764317.
KEGGiype:YPO3490.
ypk:y0694.
ypm:YP_0593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590842 Genomic DNA. Translation: CAL22078.1 .
AE009952 Genomic DNA. Translation: AAM84282.1 . Different initiation.
AE017042 Genomic DNA. Translation: AAS60863.1 . Different initiation.
PIRi AC0424.
RefSeqi NP_668031.1. NC_004088.1.
NP_991986.1. NC_005810.1.
YP_002348379.1. NC_003143.1.

3D structure databases

ProteinModelPortali Q0WBF9.
SMRi Q0WBF9. Positions 617-692.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q0WBF9. 5 interactions.
STRINGi 214092.YPO3490.

Proteomic databases

PRIDEi Q0WBF9.

Protocols and materials databases

DNASUi 1145641.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM84282 ; AAM84282 ; y0694 .
AAS60863 ; AAS60863 ; YP_0593 .
GeneIDi 1145641.
1176307.
2764317.
KEGGi ype:YPO3490.
ypk:y0694.
ypm:YP_0593.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci YPES214092:GKDD-3444-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIM10+ / Biovar Mediaevalis.
  3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.

Entry informationi

Entry nameiPNP_YERPE
AccessioniPrimary (citable) accession number: Q0WBF9
Secondary accession number(s): Q74X66, Q8D1D1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 5, 2006
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3