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Q0WBF9

- PNP_YERPE

UniProt

Q0WBF9 - PNP_YERPE

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, YPO3490, y0694, YP_0593
Organism
Yersinia pestis
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi486 – 4861Magnesium By similarity
Metal bindingi492 – 4921Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciYPES214092:GKDD-3444-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:YPO3490, y0694, YP_0593
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000815: Chromosome, UP000001019: Chromosome, UP000002490: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 705705Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000329947Add
BLAST

Proteomic databases

PRIDEiQ0WBF9.

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Protein-protein interaction databases

IntActiQ0WBF9. 5 interactions.
STRINGi214092.YPO3490.

Structurei

3D structure databases

ProteinModelPortaliQ0WBF9.
SMRiQ0WBF9. Positions 617-692.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini553 – 61260KH
Add
BLAST
Domaini622 – 69069S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0WBF9-1 [UniParc]FASTAAdd to Basket

« Hide

MLTPIIRKFQ YGQHTVTIET GMMARQATAA VMVSMDDTAV FVTVVGQKKA    50
KPGQSFFPLT VNYQERTYAA GRIPGSFFRR EGRPSEGETL TSRLIDRPIR 100
PLFPDSFLNE VQVIATVVSV NPQINPDIVA LIGASAALSL SGIPFNGPIG 150
AARVGFINDQ YVLNPTTDEL KESRLDLVVA GTAGAVLMVE SEADILSEEQ 200
MLGAVVFGHE QQQVVIENIN ALVAEAGKPK WDWQAEPVNE ALHARVAELA 250
EARLGDAYRI TEKQERYTQV DAIKADVTEA LLAQDDTLDA AEIQDILASV 300
EKNVVRSRVL RGEPRIDGRE KDMIRGLDVR TGILPRTHGS ALFTRGETQA 350
LVTATLGTAR DAQNIDELMG ERTDSFLLHY NFPPYCVGET GMVGSPKRRE 400
IGHGRLAKRG VLAVMPSASE FPYTIRVVSE ITESNGSSSM ASVCGASLAL 450
MDAGVPIKAA VAGIAMGLVK EGDNFVVLSD ILGDEDHLGD MDFKVAGSRD 500
GVTALQMDIK IEGITREIMQ VALNQAKGAR LHILGVMEQA ISTPRGDISE 550
FAPRIYTMKI NPEKIKDVIG KGGSVIRALT DETGTTIEIE DDGTIKIAAT 600
DGDKAKHAIR RIEEITAEIE VGRIYAGKVT RIVDFGAFVA IGGGKEGLVH 650
ISQIADKRVE KVTDYLQMGQ DVPVKVMEVD RQGRIRLSIK EATTPDAEAP 700
EAAAE 705
Length:705
Mass (Da):76,196
Last modified:September 5, 2006 - v1
Checksum:iE59E8F1724574722
GO

Sequence cautioni

The sequence AAM84282.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAS60863.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590842 Genomic DNA. Translation: CAL22078.1.
AE009952 Genomic DNA. Translation: AAM84282.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS60863.1. Different initiation.
PIRiAC0424.
RefSeqiNP_668031.1. NC_004088.1.
NP_991986.1. NC_005810.1.
YP_002348379.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM84282; AAM84282; y0694.
AAS60863; AAS60863; YP_0593.
GeneIDi1145641.
1176307.
2764317.
KEGGiype:YPO3490.
ypk:y0694.
ypm:YP_0593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590842 Genomic DNA. Translation: CAL22078.1 .
AE009952 Genomic DNA. Translation: AAM84282.1 . Different initiation.
AE017042 Genomic DNA. Translation: AAS60863.1 . Different initiation.
PIRi AC0424.
RefSeqi NP_668031.1. NC_004088.1.
NP_991986.1. NC_005810.1.
YP_002348379.1. NC_003143.1.

3D structure databases

ProteinModelPortali Q0WBF9.
SMRi Q0WBF9. Positions 617-692.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q0WBF9. 5 interactions.
STRINGi 214092.YPO3490.

Proteomic databases

PRIDEi Q0WBF9.

Protocols and materials databases

DNASUi 1145641.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM84282 ; AAM84282 ; y0694 .
AAS60863 ; AAS60863 ; YP_0593 .
GeneIDi 1145641.
1176307.
2764317.
KEGGi ype:YPO3490.
ypk:y0694.
ypm:YP_0593.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci YPES214092:GKDD-3444-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIM10+ / Biovar Mediaevalis.
  3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.

Entry informationi

Entry nameiPNP_YERPE
AccessioniPrimary (citable) accession number: Q0WBF9
Secondary accession number(s): Q74X66, Q8D1D1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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