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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Yersinia pestis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi486MagnesiumUniRule annotation1
Metal bindingi492MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:YPO3490, y0694, YP_0593
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003299471 – 705Polyribonucleotide nucleotidyltransferaseAdd BLAST705

Proteomic databases

PaxDbiQ0WBF9
PRIDEiQ0WBF9

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

IntActiQ0WBF9, 5 interactors
STRINGi187410.y0694

Structurei

3D structure databases

ProteinModelPortaliQ0WBF9
SMRiQ0WBF9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini553 – 612KHUniRule annotationAdd BLAST60
Domaini622 – 690S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62 Bacteria
COG1185 LUCA
HOGENOMiHOG000218326
KOiK00962
OMAiRYMHNYN

Family and domain databases

Gene3Di3.30.1370.10, 1 hit
3.30.230.70, 2 hits
HAMAPiMF_01595 PNPase, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR012340 NA-bd_OB-fold
IPR012162 PNPase
IPR027408 PNPase/RNase_PH_dom_sf
IPR015848 PNPase_PH_RNA-bd_bac/org-type
IPR036456 PNPase_PH_RNA-bd_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR022967 S1_dom
IPR003029 S1_domain
PANTHERiPTHR11252 PTHR11252, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 1 hit
PF03726 PNPase, 1 hit
PF01138 RNase_PH, 2 hits
PF03725 RNase_PH_C, 2 hits
PF00575 S1, 1 hit
PIRSFiPIRSF005499 PNPase, 1 hit
SMARTiView protein in SMART
SM00322 KH, 1 hit
SM00316 S1, 1 hit
SUPFAMiSSF46915 SSF46915, 1 hit
SSF50249 SSF50249, 1 hit
SSF54211 SSF54211, 2 hits
SSF54791 SSF54791, 1 hit
SSF55666 SSF55666, 2 hits
TIGRFAMsiTIGR03591 polynuc_phos, 1 hit
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 1 hit
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

Q0WBF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTPIIRKFQ YGQHTVTIET GMMARQATAA VMVSMDDTAV FVTVVGQKKA
60 70 80 90 100
KPGQSFFPLT VNYQERTYAA GRIPGSFFRR EGRPSEGETL TSRLIDRPIR
110 120 130 140 150
PLFPDSFLNE VQVIATVVSV NPQINPDIVA LIGASAALSL SGIPFNGPIG
160 170 180 190 200
AARVGFINDQ YVLNPTTDEL KESRLDLVVA GTAGAVLMVE SEADILSEEQ
210 220 230 240 250
MLGAVVFGHE QQQVVIENIN ALVAEAGKPK WDWQAEPVNE ALHARVAELA
260 270 280 290 300
EARLGDAYRI TEKQERYTQV DAIKADVTEA LLAQDDTLDA AEIQDILASV
310 320 330 340 350
EKNVVRSRVL RGEPRIDGRE KDMIRGLDVR TGILPRTHGS ALFTRGETQA
360 370 380 390 400
LVTATLGTAR DAQNIDELMG ERTDSFLLHY NFPPYCVGET GMVGSPKRRE
410 420 430 440 450
IGHGRLAKRG VLAVMPSASE FPYTIRVVSE ITESNGSSSM ASVCGASLAL
460 470 480 490 500
MDAGVPIKAA VAGIAMGLVK EGDNFVVLSD ILGDEDHLGD MDFKVAGSRD
510 520 530 540 550
GVTALQMDIK IEGITREIMQ VALNQAKGAR LHILGVMEQA ISTPRGDISE
560 570 580 590 600
FAPRIYTMKI NPEKIKDVIG KGGSVIRALT DETGTTIEIE DDGTIKIAAT
610 620 630 640 650
DGDKAKHAIR RIEEITAEIE VGRIYAGKVT RIVDFGAFVA IGGGKEGLVH
660 670 680 690 700
ISQIADKRVE KVTDYLQMGQ DVPVKVMEVD RQGRIRLSIK EATTPDAEAP

EAAAE
Length:705
Mass (Da):76,196
Last modified:September 5, 2006 - v1
Checksum:iE59E8F1724574722
GO

Sequence cautioni

The sequence AAM84282 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAS60863 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL22078.1
AE009952 Genomic DNA Translation: AAM84282.1 Different initiation.
AE017042 Genomic DNA Translation: AAS60863.1 Different initiation.
PIRiAC0424
RefSeqiWP_002209259.1, NZ_PDBR01000021.1
YP_002348379.1, NC_003143.1

Genome annotation databases

EnsemblBacteriaiAAM84282; AAM84282; y0694
AAS60863; AAS60863; YP_0593
GeneIDi1176307
KEGGiype:YPO3490
ypk:y0694
ypm:YP_0593
PATRICifig|214092.21.peg.3984

Similar proteinsi

Entry informationi

Entry nameiPNP_YERPE
AccessioniPrimary (citable) accession number: Q0WBF9
Secondary accession number(s): Q74X66, Q8D1D1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 5, 2006
Last modified: March 28, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health