ID   GHRB_YERPE              Reviewed;         326 AA.
AC   Q0W9V5; Q74PC9; Q8CZG7;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   16-JUN-2009, entry version 32.
DE   RecName: Full=Glyoxylate/hydroxypyruvate reductase B;
DE            EC=1.1.1.79;
DE            EC=1.1.1.81;
GN   Name=ghrB; OrderedLocusNames=YPO4078, y4096, YP_3988;
OS   Yersinia pestis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Yersinia.
OX   NCBI_TaxID=632;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CO-92 / Biovar Orientalis;
RX   MEDLINE=21470413; PubMed=11586360; DOI=10.1038/35097083;
RA   Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA   Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA   Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA   Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G.,
RA   Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V.,
RA   Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M.,
RA   Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G.;
RT   "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL   Nature 413:523-527(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KIM5 / Biovar Mediaevalis;
RX   MEDLINE=22137863; PubMed=12142430;
RX   DOI=10.1128/JB.184.16.4601-4611.2002;
RA   Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA   Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C.,
RA   Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V.,
RA   Straley S.C., McDonough K.A., Nilles M.L., Matson J.S., Blattner F.R.,
RA   Perry R.D.;
RT   "Genome sequence of Yersinia pestis KIM.";
RL   J. Bacteriol. 184:4601-4611(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=91001 / Biovar Mediaevalis;
RX   PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA   Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA   Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z.,
RA   Jin L., Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J.,
RA   Yang H., Wang J., Huang P., Yang R.;
RT   "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT   avirulent to humans.";
RL   DNA Res. 11:179-197(2004).
CC   -!- FUNCTION: Catalyzes the NADPH-dependent reduction of glyoxylate
CC       and hydroxypyruvate into glycolate and glycerate, respectively (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Glycolate + NADP(+) = glyoxylate + NADPH.
CC   -!- CATALYTIC ACTIVITY: D-glycerate + NAD(P)(+) = hydroxypyruvate +
CC       NAD(P)H.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid
CC       dehydrogenase family. GhrB subfamily.
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DR   EMBL; AL590842; CAL22649.1; -; Genomic_DNA.
DR   EMBL; AE009952; AAM87639.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AE017042; AAS64128.1; ALT_INIT; Genomic_DNA.
DR   PIR; AF0495; AF0495.
DR   RefSeq; NP_671388.2; -.
DR   RefSeq; NP_995251.2; -.
DR   RefSeq; YP_002348933.1; -.
DR   GeneID; 1149043; -.
DR   GeneID; 1176909; -.
DR   GeneID; 2763590; -.
DR   GenomeReviews; AE009952_GR; y4096.
DR   GenomeReviews; AE017042_GR; YP_3988.
DR   GenomeReviews; AL590842_GR; YPO4078.
DR   KEGG; ype:YPO4078; -.
DR   KEGG; ypk:y4096; -.
DR   KEGG; ypm:YP_3988; -.
DR   HOGENOM; Q0W9V5; -.
DR   OMA; Q0W9V5; KVYIAEP.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:HAMAP.
DR   GO; GO:0030267; F:glyoxylate reductase (NADP) activity; IEA:HAMAP.
DR   GO; GO:0016618; F:hydroxypyruvate reductase activity; IEA:HAMAP.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01667; -; 1.
DR   InterPro; IPR006139; D-isomer_2_OHA_DH.
DR   InterPro; IPR006140; D-isomer_2_OHA_DH_NAD-bd.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00389; 2-Hacid_dh; 1.
DR   Pfam; PF02826; 2-Hacid_dh_C; 1.
DR   PROSITE; PS00065; D_2_HYDROXYACID_DH_1; 1.
DR   PROSITE; PS00670; D_2_HYDROXYACID_DH_2; FALSE_NEG.
DR   PROSITE; PS00671; D_2_HYDROXYACID_DH_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; NAD; NADP; Oxidoreductase.
FT   CHAIN         1    326       Glyoxylate/hydroxypyruvate reductase B.
FT                                /FTId=PRO_0000348406.
FT   ACT_SITE    237    237       By similarity.
FT   ACT_SITE    266    266       By similarity.
FT   ACT_SITE    285    285       Proton donor (By similarity).
SQ   SEQUENCE   326 AA;  35465 MW;  935B405A48250AE2 CRC64;
     MKPSIVLYKS IPTDLHQRLA QHFTVNSFDG LTPDNQPELL AALQQAEGLI GSGGKIDQDF
     LQLAPNLRAA STISVGYDNF DVEALSQRGI ALMHTPTVLT ETVADTMMAL MLSTARRVVE
     LAERVKAGEW QESIGDDWFG VDVHHKTIGI LGMGRIGMAL AQRAHFGFSM PVLYTSRRPH
     EAAEQRFGAR HCSLDTLLAE ADFLCITLPM TEQTYHMIGR EQLAKMKSSA ILINAGRGPV
     VDEQALIAAL QDGTIHAAGL DVFEQEPLPV DSPLLTLRNV VAVPHIGSAT HETRYNMAAC
     AVDNLINALT GTVKENCVNP QVLITH
//