Reviewed, UniProtKB/Swiss-Prot Q0W9V5 (GHRB_YERPE)

Names and origin

Protein names

Recommended name:
    Glyoxylate/hydroxypyruvate reductase B
    EC=1.1.1.79
    EC=1.1.1.81

Gene names

Name:	ghrB
Ordered Locus Names:	YPO4078, y4096, YP_3988

Organism

Yersinia pestis [Complete proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity.
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01667 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01667
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Glyoxylate/hydroxypyruvate reductase B HAMAP MF_01667		PRO_0000348406
Sites
	Active site	237	1	By similarity
	Active site	266	1	By similarity
	Active site	285	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0W9V5-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 935B405A48250AE2	FASTA	326	35,465
	10 20 30 40 50 60 MKPSIVLYKS IPTDLHQRLA QHFTVNSFDG LTPDNQPELL AALQQAEGLI GSGGKIDQDF 70 80 90 100 110 120 LQLAPNLRAA STISVGYDNF DVEALSQRGI ALMHTPTVLT ETVADTMMAL MLSTARRVVE 130 140 150 160 170 180 LAERVKAGEW QESIGDDWFG VDVHHKTIGI LGMGRIGMAL AQRAHFGFSM PVLYTSRRPH 190 200 210 220 230 240 EAAEQRFGAR HCSLDTLLAE ADFLCITLPM TEQTYHMIGR EQLAKMKSSA ILINAGRGPV 250 260 270 280 290 300 VDEQALIAAL QDGTIHAAGL DVFEQEPLPV DSPLLTLRNV VAVPHIGSAT HETRYNMAAC 310 320 AVDNLINALT GTVKENCVNP QVLITH

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL590842 Genomic DNA. Translation: CAL22649.1. AE009952 Genomic DNA. Translation: AAM87639.1. Different initiation. AE017042 Genomic DNA. Translation: AAS64128.1. Different initiation.
PIR	AF0495.
RefSeq	NP_671388.2. NP_995251.2. YP_002348933.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1149043. 1176909. 2763590.
GenomeReviews	Gene locus y4096 in contig AE009952_GR. Gene locus YP_3988 in contig AE017042_GR. Gene locus YPO4078 in contig AL590842_GR.
KEGG	ype:YPO4078. ypk:y4096. ypm:YP_3988.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0W9V5.
OMA	Q0W9V5. KVYIAEP.
Family and domain databases
HAMAP	MF_01667. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRB_YERPE

Accession

Primary (citable) accession number: Q0W9V5
Secondary accession number(s): Q74PC9, Q8CZG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)