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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciUMET351160:GJT4-2973-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:UNCMA_28940
ORF Names:LRC306
OrganismiMethanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50)
Taxonomic identifieri351160 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanocellalesMethanocellaceaeMethanocella
ProteomesiUP000000663 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Glutamate--tRNA ligasePRO_0000367807Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi351160.LRC306.

Structurei

3D structure databases

ProteinModelPortaliQ0W8L2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi112 – 12211"HIGH" regionAdd
BLAST

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000073585.
KOiK01885.
OMAiMRFAPNP.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A.
InterProiIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0W8L2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METKDIEILI QKFALQNAYK HGSVPQAGAV TGKLLGTHPE LRPHAKELMP
60 70 80 90 100
IVQKVLADIG QMSQDEIKAK LSEIAPELIE ELSVKKEVRR GLPPLDTSML
110 120 130 140 150
KPGQKVTLRI APNPNGPPSL GNARGIIVNH EYARMYDGVF IMRFDDTDPS
160 170 180 190 200
IKKPMIEAYT WYVEHAKWLG CPPDKVVAAS DRLPLYYEQA EKLIDLGKAY
210 220 230 240 250
VCTCDNEVFH DLKEAGKPCP HRETPPAENM EKWKKMLAGG YGGKEAVLRI
260 270 280 290 300
KTDIAHKDPA MRDWVAFRIV TEPHPKTGTK YMVWPMLDFE SAMEDHFLGV
310 320 330 340 350
THIIRGKDLM KTADKQKYIY RYLGWEYPHV SHWGRVRLLG FGKFSTSVMK
360 370 380 390 400
KGIEAGEYRG WDDPQLPTVV ALKRRGIEPE AIRNVMINMG VTETDIEFSM
410 420 430 440 450
DTLYAENRKI VDPKANRYFF VPDPVVLKVN GAPFTTAKAP LHPQDHKRGF
460 470 480 490 500
REMCVAENPE ILIPKSDADN ARPGDILRLK DLYNVRITGS DPLTGDYIGN
510 520 530 540 550
DLSVLKQGAK IVQWVTREGG VPTRVIGPDG EFHGIAECDI RNELNNVVQF
560 570
ERFAFVRIDT INGVVLAYYT HP
Length:572
Mass (Da):64,577
Last modified:September 5, 2006 - v1
Checksum:i286BE9D8D32127DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM114193 Genomic DNA. Translation: CAJ35281.1.
RefSeqiWP_012037209.1. NC_009464.1.
YP_684607.1. NC_009464.1.

Genome annotation databases

EnsemblBacteriaiCAJ35281; CAJ35281; LRC306.
GeneIDi5143841.
KEGGirci:LRC306.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM114193 Genomic DNA. Translation: CAJ35281.1.
RefSeqiWP_012037209.1. NC_009464.1.
YP_684607.1. NC_009464.1.

3D structure databases

ProteinModelPortaliQ0W8L2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351160.LRC306.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ35281; CAJ35281; LRC306.
GeneIDi5143841.
KEGGirci:LRC306.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000073585.
KOiK01885.
OMAiMRFAPNP.

Enzyme and pathway databases

BioCyciUMET351160:GJT4-2973-MONOMER.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A.
InterProiIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere."
    Erkel C., Kube M., Reinhardt R., Liesack W.
    Science 313:370-372(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiSYE_METAR
AccessioniPrimary (citable) accession number: Q0W8L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 5, 2006
Last modified: July 22, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.