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Q0W8L2 (SYE_UNCMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:UNCMA_28940
ORF Names:LRC306
OrganismUncultured methanogenic archaeon RC-I [Complete proteome] [HAMAP]
Taxonomic identifier351160 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanocellalesMethanocellaceaeMethanocella

Protein attributes

Sequence length572 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 572572Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000367807

Regions

Motif112 – 12211"HIGH" region HAMAP-Rule MF_00022

Sequences

Sequence LengthMass (Da)Tools
Q0W8L2 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 286BE9D8D32127DC

FASTA57264,577
        10         20         30         40         50         60 
METKDIEILI QKFALQNAYK HGSVPQAGAV TGKLLGTHPE LRPHAKELMP IVQKVLADIG 

        70         80         90        100        110        120 
QMSQDEIKAK LSEIAPELIE ELSVKKEVRR GLPPLDTSML KPGQKVTLRI APNPNGPPSL 

       130        140        150        160        170        180 
GNARGIIVNH EYARMYDGVF IMRFDDTDPS IKKPMIEAYT WYVEHAKWLG CPPDKVVAAS 

       190        200        210        220        230        240 
DRLPLYYEQA EKLIDLGKAY VCTCDNEVFH DLKEAGKPCP HRETPPAENM EKWKKMLAGG 

       250        260        270        280        290        300 
YGGKEAVLRI KTDIAHKDPA MRDWVAFRIV TEPHPKTGTK YMVWPMLDFE SAMEDHFLGV 

       310        320        330        340        350        360 
THIIRGKDLM KTADKQKYIY RYLGWEYPHV SHWGRVRLLG FGKFSTSVMK KGIEAGEYRG 

       370        380        390        400        410        420 
WDDPQLPTVV ALKRRGIEPE AIRNVMINMG VTETDIEFSM DTLYAENRKI VDPKANRYFF 

       430        440        450        460        470        480 
VPDPVVLKVN GAPFTTAKAP LHPQDHKRGF REMCVAENPE ILIPKSDADN ARPGDILRLK 

       490        500        510        520        530        540 
DLYNVRITGS DPLTGDYIGN DLSVLKQGAK IVQWVTREGG VPTRVIGPDG EFHGIAECDI 

       550        560        570 
RNELNNVVQF ERFAFVRIDT INGVVLAYYT HP 

« Hide

References

[1]"Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere."
Erkel C., Kube M., Reinhardt R., Liesack W.
Science 313:370-372(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM114193 Genomic DNA. Translation: CAJ35281.1.
RefSeqYP_684607.1. NC_009464.1.

3D structure databases

ProteinModelPortalQ0W8L2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351160.LRC306.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ35281; CAJ35281; LRC306.
GeneID5143841.
KEGGrci:LRC306.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000073585.
KOK01885.
OMAYLGWDDP.

Enzyme and pathway databases

BioCycUMET351160:GJT4-2973-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_A. Glu_tRNA_synth_A.
InterProIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00463. gltX_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_UNCMA
AccessionPrimary (citable) accession number: Q0W8L2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries