Reviewed, UniProtKB/Swiss-Prot Q0W7E0 (ASPD_UNCMA)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	UNCMA_24780
ORF Names:	RCIX226

Organism

Uncultured methanogenic archaeon RC-I [Complete proteome] [HAMAP]

Taxonomic identifier

351160 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › environmental samples

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity.
Catalytic activity	L-aspartate + H(2)O + NAD(P)(+) = oxaloacetate + NH(3) + NAD(P)H.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1.
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: HAMAP NADP binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Probable L-aspartate dehydrogenase		PRO_1000067314
Sites
	Active site	221	1	By similarity
	Binding site	123	1	NAD; via amide nitrogen By similarity
	Binding site	191	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0W7E0-1 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 62A6113535FAE704	FASTA	270	28,621
	10 20 30 40 50 60 MFRIGIVGAG AIGKEIARAI DNGTVPAKLE AIYDRDTAEA TSFAASLKSK PRVLPLEELV 70 80 90 100 110 120 EASNFVVEAA AQSAVREVAI AALSRSRSVM IMSVGALADK ELLETIRTMA KEHCCSIYLP 130 140 150 160 170 180 SGAIGGLDAV KAASICKIDS VTITTRKPRD GLRGAPFIVR NNIDVDSMDE PTEIFSGPAA 190 200 210 220 230 240 VAIKEFPANV NVAASLSLVG IGFERTLVRV VVDPTIKRNI HEISVRGEFG ELHTVVENVP 250 260 270 ARSNPKTSFL AALSAIATLR QVCEPLKIGT

References

[1]	"Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere." Erkel C., Kube M., Reinhardt R., Liesack W. Science 313:370-372(2006) [PubMed: 16857943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM114193 Genomic DNA. Translation: CAJ35703.1.
RefSeq	YP_685029.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5143303.
GenomeReviews	Gene locus UNCMA_24780 in contig AM114193_GR.
KEGG	rci:RCIX226.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01265. [Tree]
InterPro	IPR005106. Asp/hSer_DHase_NAD-bd. IPR002811. Asp_DHase. IPR011182. Asp_DHase_NAD_syn. [Graphical view]
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProDom	PD017325. Asp_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ASPD_UNCMA

Accession

Primary (citable) accession number: Q0W7E0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 31, 2006
Last modified:	November 25, 2008
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)