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Protein

Probable L-aspartate dehydrogenase

Gene

nadX

Organism
Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.UniRule annotation

Catalytic activityi

L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H.UniRule annotation

Pathway: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes iminoaspartate from L-aspartate (dehydrogenase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Probable L-aspartate dehydrogenase (nadX)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes iminoaspartate from L-aspartate (dehydrogenase route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei123 – 1231NAD; via amide nitrogenUniRule annotation
Binding sitei191 – 1911NADUniRule annotation
Active sitei221 – 2211UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BioCyciUMET351160:GJT4-2542-MONOMER.
UniPathwayiUPA00253; UER00456.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable L-aspartate dehydrogenaseUniRule annotation (EC:1.4.1.21UniRule annotation)
Gene namesi
Name:nadXUniRule annotation
Ordered Locus Names:UNCMA_24780
ORF Names:RCIX226
OrganismiMethanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50)
Taxonomic identifieri351160 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanocellalesMethanocellaceaeMethanocella
ProteomesiUP000000663 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270Probable L-aspartate dehydrogenasePRO_1000067314Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi351160.RCIX226.

Structurei

3D structure databases

ProteinModelPortaliQ0W7E0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the L-aspartate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1712.
HOGENOMiHOG000206326.
KOiK06989.
OMAiPKTSYLA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01265. NadX.
InterProiIPR005106. Asp/hSer_DH_NAD-bd.
IPR002811. Asp_DH.
IPR022487. Asp_DH_arc.
IPR020626. Asp_DH_prok.
IPR011182. L-Asp_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01958. DUF108. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFiPIRSF005227. Asp_dh_NAD_syn. 1 hit.
TIGRFAMsiTIGR03855. NAD_NadX. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0W7E0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRIGIVGAG AIGKEIARAI DNGTVPAKLE AIYDRDTAEA TSFAASLKSK
60 70 80 90 100
PRVLPLEELV EASNFVVEAA AQSAVREVAI AALSRSRSVM IMSVGALADK
110 120 130 140 150
ELLETIRTMA KEHCCSIYLP SGAIGGLDAV KAASICKIDS VTITTRKPRD
160 170 180 190 200
GLRGAPFIVR NNIDVDSMDE PTEIFSGPAA VAIKEFPANV NVAASLSLVG
210 220 230 240 250
IGFERTLVRV VVDPTIKRNI HEISVRGEFG ELHTVVENVP ARSNPKTSFL
260 270
AALSAIATLR QVCEPLKIGT
Length:270
Mass (Da):28,621
Last modified:October 31, 2006 - v1
Checksum:i62A6113535FAE704
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM114193 Genomic DNA. Translation: CAJ35703.1.
RefSeqiWP_012036796.1. NC_009464.1.
YP_685029.1. NC_009464.1.

Genome annotation databases

EnsemblBacteriaiCAJ35703; CAJ35703; RCIX226.
GeneIDi5143303.
KEGGirci:RCIX226.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM114193 Genomic DNA. Translation: CAJ35703.1.
RefSeqiWP_012036796.1. NC_009464.1.
YP_685029.1. NC_009464.1.

3D structure databases

ProteinModelPortaliQ0W7E0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351160.RCIX226.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ35703; CAJ35703; RCIX226.
GeneIDi5143303.
KEGGirci:RCIX226.

Phylogenomic databases

eggNOGiCOG1712.
HOGENOMiHOG000206326.
KOiK06989.
OMAiPKTSYLA.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00456.
BioCyciUMET351160:GJT4-2542-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01265. NadX.
InterProiIPR005106. Asp/hSer_DH_NAD-bd.
IPR002811. Asp_DH.
IPR022487. Asp_DH_arc.
IPR020626. Asp_DH_prok.
IPR011182. L-Asp_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01958. DUF108. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFiPIRSF005227. Asp_dh_NAD_syn. 1 hit.
TIGRFAMsiTIGR03855. NAD_NadX. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere."
    Erkel C., Kube M., Reinhardt R., Liesack W.
    Science 313:370-372(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiASPD_METAR
AccessioniPrimary (citable) accession number: Q0W7E0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 31, 2006
Last modified: June 24, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.