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Q0W720

- SYI_UNCMA

UniProt

Q0W720 - SYI_UNCMA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Uncultured methanogenic archaeon RC-I
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei647 – 6471ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciUMET351160:GJT4-2419-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:UNCMA_23590
    ORF Names:RCIX379
    OrganismiUncultured methanogenic archaeon RC-I
    Taxonomic identifieri351160 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanocellalesMethanocellaceaeMethanocella
    ProteomesiUP000000663: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11041104Isoleucine--tRNA ligasePRO_1000022161Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi351160.RCIX379.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0W720.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi644 – 6485"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiERLMLHQ.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0W720-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIQEEKEQYS SKSLEAGVDE LWRASDAYAL TRKKRLGSKR FFFVDGPPYT     50
    TGRIHLGTAW NKIIKDSVLR YRSMNGYDLI DRPGWDMHGL PIEVKVESIL 100
    GFKTKKDIEE FGVARFTEEC KKFAIGNMHE MTSQFKKLGV WMNWDDPYMT 150
    LKNEYIEAAW WTIKQAHEKH LLERGLRNVN WCPRCETAIA DSEVEYADRK 200
    DDSIYVKFPL KNEEGFLVIW TTTPWTIPAN MAVAANKDFT YAMVYALPAA 250
    VLEEAAMQAG LDHTTLVEHH SDGRPKPMRY SDKVAKMKEA IGEEKVKELY 300
    EKHGEKLIIA RDLVDGVLKM GRYADYQVLK TMTGEELKGT EYVHPLADLI 350
    PCQKETEHKV YLADFVVGEN TGMVHIAPGH GFDDFELGLK EGIRAYCPVK 400
    ANGHYDDSVG AYAGMEIREA NPKIMEDLRQ RNLLLGATTI EHRYGHCWRC 450
    KTPIIFLTTD QWFIAVSKMK EDMLAEVKRV NWYPDWAGSA RFYDWVNGAR 500
    DWCVSRQRYW GIPIPIWKCE KCGSLDVIGT KEELERKVGR EVPDLHRPFV 550
    DEVRLECECG GSMRRVEDIF DVWFDSAVAS WATLHFPGRK DLMDWWPADF 600
    IVEGHDQTRG WFYSQLGAGM VGFGKAPYNG VCMHGFTLDE TGKKMSKSLG 650
    NVVAPEDVVE KLGADTLRLY VLSQNAPWED LSFSWEECGN INRAINIFWN 700
    VYRFPLPYMV LDKFDPAKVT LESVKGSLRV EDRWILSKLQ AVIKDVDTYM 750
    ATYELHRATR SIISFILEDL SRWYVQLARE RTWVEADDPD KLAAYRVLYD 800
    TLVTTVKLIA PFTPYIAERM YQNLVRNVSA EAPVSVHMCD WPVVDSSLLD 850
    EQLNRDMDIA RKIVEASSNA RQKAKRKLRW PVQKIVVAAE NPDVVTAVKD 900
    LSGVIGEQTN SKEVVVLAPG EANTELGVEV VPNPKLIGPI FKAVAGKVTA 950
    ALKEADGRAV KKAIEEEKKY IVEIPEGSFD ILLDMVSFRD VIPESLAMAD 1000
    FPGGKVYVDV TLSKELEAEG YTRELIRRIQ DMRKEMNLNV EDRIKVEVYV 1050
    GDEKVLDLVK SMKDYAAGEV RADTLDLKTE KPAAGFVKDW DVEGIPMTIG 1100
    LEKI 1104
    Length:1,104
    Mass (Da):125,895
    Last modified:September 5, 2006 - v1
    Checksum:i5958154F4B06387E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM114193 Genomic DNA. Translation: CAJ35823.1.
    RefSeqiYP_685149.1. NC_009464.1.

    Genome annotation databases

    EnsemblBacteriaiCAJ35823; CAJ35823; RCIX379.
    GeneIDi5145666.
    KEGGirci:RCIX379.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM114193 Genomic DNA. Translation: CAJ35823.1 .
    RefSeqi YP_685149.1. NC_009464.1.

    3D structure databases

    ProteinModelPortali Q0W720.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 351160.RCIX379.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAJ35823 ; CAJ35823 ; RCIX379 .
    GeneIDi 5145666.
    KEGGi rci:RCIX379.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi ERLMLHQ.

    Enzyme and pathway databases

    BioCyci UMET351160:GJT4-2419-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere."
      Erkel C., Kube M., Reinhardt R., Liesack W.
      Science 313:370-372(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiSYI_UNCMA
    AccessioniPrimary (citable) accession number: Q0W720
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3