Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q0W720 (SYI_UNCMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:UNCMA_23590
ORF Names:RCIX379
OrganismUncultured methanogenic archaeon RC-I [Complete proteome] [HAMAP]
Taxonomic identifier351160 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanocellales › Methanocellaceae › Methanocella ›

Protein attributes

Sequence length1104 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003

Cofactor

Zinc By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11041104Isoleucine--tRNA ligase HAMAP-Rule MF_02003
PRO_1000022161

Regions

Motif48 – 5811"HIGH" region HAMAP-Rule MF_02003
Motif644 – 6485"KMSKS" region HAMAP-Rule MF_02003

Sites

Binding site6471ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0W720 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 5958154F4B06387E

FASTA1,104125,895
        10         20         30         40         50         60 
MIQEEKEQYS SKSLEAGVDE LWRASDAYAL TRKKRLGSKR FFFVDGPPYT TGRIHLGTAW 

        70         80         90        100        110        120 
NKIIKDSVLR YRSMNGYDLI DRPGWDMHGL PIEVKVESIL GFKTKKDIEE FGVARFTEEC 

       130        140        150        160        170        180 
KKFAIGNMHE MTSQFKKLGV WMNWDDPYMT LKNEYIEAAW WTIKQAHEKH LLERGLRNVN 

       190        200        210        220        230        240 
WCPRCETAIA DSEVEYADRK DDSIYVKFPL KNEEGFLVIW TTTPWTIPAN MAVAANKDFT 

       250        260        270        280        290        300 
YAMVYALPAA VLEEAAMQAG LDHTTLVEHH SDGRPKPMRY SDKVAKMKEA IGEEKVKELY 

       310        320        330        340        350        360 
EKHGEKLIIA RDLVDGVLKM GRYADYQVLK TMTGEELKGT EYVHPLADLI PCQKETEHKV 

       370        380        390        400        410        420 
YLADFVVGEN TGMVHIAPGH GFDDFELGLK EGIRAYCPVK ANGHYDDSVG AYAGMEIREA 

       430        440        450        460        470        480 
NPKIMEDLRQ RNLLLGATTI EHRYGHCWRC KTPIIFLTTD QWFIAVSKMK EDMLAEVKRV 

       490        500        510        520        530        540 
NWYPDWAGSA RFYDWVNGAR DWCVSRQRYW GIPIPIWKCE KCGSLDVIGT KEELERKVGR 

       550        560        570        580        590        600 
EVPDLHRPFV DEVRLECECG GSMRRVEDIF DVWFDSAVAS WATLHFPGRK DLMDWWPADF 

       610        620        630        640        650        660 
IVEGHDQTRG WFYSQLGAGM VGFGKAPYNG VCMHGFTLDE TGKKMSKSLG NVVAPEDVVE 

       670        680        690        700        710        720 
KLGADTLRLY VLSQNAPWED LSFSWEECGN INRAINIFWN VYRFPLPYMV LDKFDPAKVT 

       730        740        750        760        770        780 
LESVKGSLRV EDRWILSKLQ AVIKDVDTYM ATYELHRATR SIISFILEDL SRWYVQLARE 

       790        800        810        820        830        840 
RTWVEADDPD KLAAYRVLYD TLVTTVKLIA PFTPYIAERM YQNLVRNVSA EAPVSVHMCD 

       850        860        870        880        890        900 
WPVVDSSLLD EQLNRDMDIA RKIVEASSNA RQKAKRKLRW PVQKIVVAAE NPDVVTAVKD 

       910        920        930        940        950        960 
LSGVIGEQTN SKEVVVLAPG EANTELGVEV VPNPKLIGPI FKAVAGKVTA ALKEADGRAV 

       970        980        990       1000       1010       1020 
KKAIEEEKKY IVEIPEGSFD ILLDMVSFRD VIPESLAMAD FPGGKVYVDV TLSKELEAEG 

      1030       1040       1050       1060       1070       1080 
YTRELIRRIQ DMRKEMNLNV EDRIKVEVYV GDEKVLDLVK SMKDYAAGEV RADTLDLKTE 

      1090       1100 
KPAAGFVKDW DVEGIPMTIG LEKI

« Hide

References

[1]"Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere."
Erkel C., Kube M., Reinhardt R., Liesack W.
Science 313:370-372(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM114193 Genomic DNA. Translation: CAJ35823.1.
RefSeqYP_685149.1. NC_009464.1.

3D structure databases

ProteinModelPortalQ0W720.
ModBaseSearch...

Protein-protein interaction databases

STRING351160.RCIX379.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ35823; CAJ35823; RCIX379.
GeneID5145666.
KEGGrci:RCIX379.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246403.
KOK01870.
OMAWFYSQLG.
ProtClustDBPRK06039.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPMF_02003. Ile_tRNA_synth_type2.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 2 hits.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_UNCMA
AccessionPrimary (citable) accession number: Q0W720
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents