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Q0W720

- SYI_UNCMA

UniProt

Q0W720 - SYI_UNCMA

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Protein
Isoleucine--tRNA ligase
Gene
ileS, UNCMA_23590, RCIX379
Organism
Uncultured methanogenic archaeon RC-I
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei647 – 6471ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciUMET351160:GJT4-2419-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:UNCMA_23590
ORF Names:RCIX379
OrganismiUncultured methanogenic archaeon RC-I
Taxonomic identifieri351160 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanocellalesMethanocellaceaeMethanocella
ProteomesiUP000000663: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11041104Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022161Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi351160.RCIX379.

Structurei

3D structure databases

ProteinModelPortaliQ0W720.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotation
Add
BLAST
Motifi644 – 6485"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiERLMLHQ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0W720-1 [UniParc]FASTAAdd to Basket

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MIQEEKEQYS SKSLEAGVDE LWRASDAYAL TRKKRLGSKR FFFVDGPPYT     50
TGRIHLGTAW NKIIKDSVLR YRSMNGYDLI DRPGWDMHGL PIEVKVESIL 100
GFKTKKDIEE FGVARFTEEC KKFAIGNMHE MTSQFKKLGV WMNWDDPYMT 150
LKNEYIEAAW WTIKQAHEKH LLERGLRNVN WCPRCETAIA DSEVEYADRK 200
DDSIYVKFPL KNEEGFLVIW TTTPWTIPAN MAVAANKDFT YAMVYALPAA 250
VLEEAAMQAG LDHTTLVEHH SDGRPKPMRY SDKVAKMKEA IGEEKVKELY 300
EKHGEKLIIA RDLVDGVLKM GRYADYQVLK TMTGEELKGT EYVHPLADLI 350
PCQKETEHKV YLADFVVGEN TGMVHIAPGH GFDDFELGLK EGIRAYCPVK 400
ANGHYDDSVG AYAGMEIREA NPKIMEDLRQ RNLLLGATTI EHRYGHCWRC 450
KTPIIFLTTD QWFIAVSKMK EDMLAEVKRV NWYPDWAGSA RFYDWVNGAR 500
DWCVSRQRYW GIPIPIWKCE KCGSLDVIGT KEELERKVGR EVPDLHRPFV 550
DEVRLECECG GSMRRVEDIF DVWFDSAVAS WATLHFPGRK DLMDWWPADF 600
IVEGHDQTRG WFYSQLGAGM VGFGKAPYNG VCMHGFTLDE TGKKMSKSLG 650
NVVAPEDVVE KLGADTLRLY VLSQNAPWED LSFSWEECGN INRAINIFWN 700
VYRFPLPYMV LDKFDPAKVT LESVKGSLRV EDRWILSKLQ AVIKDVDTYM 750
ATYELHRATR SIISFILEDL SRWYVQLARE RTWVEADDPD KLAAYRVLYD 800
TLVTTVKLIA PFTPYIAERM YQNLVRNVSA EAPVSVHMCD WPVVDSSLLD 850
EQLNRDMDIA RKIVEASSNA RQKAKRKLRW PVQKIVVAAE NPDVVTAVKD 900
LSGVIGEQTN SKEVVVLAPG EANTELGVEV VPNPKLIGPI FKAVAGKVTA 950
ALKEADGRAV KKAIEEEKKY IVEIPEGSFD ILLDMVSFRD VIPESLAMAD 1000
FPGGKVYVDV TLSKELEAEG YTRELIRRIQ DMRKEMNLNV EDRIKVEVYV 1050
GDEKVLDLVK SMKDYAAGEV RADTLDLKTE KPAAGFVKDW DVEGIPMTIG 1100
LEKI 1104
Length:1,104
Mass (Da):125,895
Last modified:September 5, 2006 - v1
Checksum:i5958154F4B06387E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM114193 Genomic DNA. Translation: CAJ35823.1.
RefSeqiYP_685149.1. NC_009464.1.

Genome annotation databases

EnsemblBacteriaiCAJ35823; CAJ35823; RCIX379.
GeneIDi5145666.
KEGGirci:RCIX379.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM114193 Genomic DNA. Translation: CAJ35823.1 .
RefSeqi YP_685149.1. NC_009464.1.

3D structure databases

ProteinModelPortali Q0W720.
ModBasei Search...

Protein-protein interaction databases

STRINGi 351160.RCIX379.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAJ35823 ; CAJ35823 ; RCIX379 .
GeneIDi 5145666.
KEGGi rci:RCIX379.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ERLMLHQ.

Enzyme and pathway databases

BioCyci UMET351160:GJT4-2419-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere."
    Erkel C., Kube M., Reinhardt R., Liesack W.
    Science 313:370-372(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiSYI_UNCMA
AccessioniPrimary (citable) accession number: Q0W720
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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