Reviewed, UniProtKB/Swiss-Prot Q0W700 (GUAAA_UNCMA)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit A
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	UNCMA_23390
ORF Names:	RCIX404

Organism

Uncultured methanogenic archaeon RC-I [Complete proteome] [HAMAP]

Taxonomic identifier

351160 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › environmental samples

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000292198
Regions
	Domain	5 – 189	185	Glutamine amidotransferase type-1
Sites
	Active site	78	1	Nucleophile By similarity
	Active site	165	1	By similarity
	Active site	167	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0W700-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 75B3409BB909D228	FASTA	189	20,982
	10 20 30 40 50 60 MDKLKLLVIN NYGQTCHLIH RSLRDLGMDA KLVSNTSTVE DILKQSPDGL VFSGGPTMER 70 80 90 100 110 120 AGNGENLIKI DLPILGICLG HQLMAKAYGG EIRTGQYGGF ARVDIDIVEE DDILRGIGPK 130 140 150 160 170 180 TVVWASHQDE VAKLPPDFVL LAKSNICDIE AMRHKTKPLF GVQWHPEVSH TEKGTLLLQN FIEVCRQRK

References

[1]	"Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere." Erkel C., Kube M., Reinhardt R., Liesack W. Science 313:370-372(2006) [PubMed: 16857943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM114193 Genomic DNA. Translation: CAJ35843.1.
RefSeq	YP_685169.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0W700.
Genome annotation databases
GeneID	5145228.
GenomeReviews	Gene locus UNCMA_23390 in contig AM114193_GR.
KEGG	rci:RCIX404.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GQYVHRI.
Family and domain databases
HAMAP	MF_01510. [Tree]
InterPro	IPR006220. Anth_synthII. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR004739. GMP_synth_N. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00096. GATASE.
TIGRFAMs	TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_UNCMA

Accession

Primary (citable) accession number: Q0W700

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	September 5, 2006
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)