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Q0W6Q4 (PUR2_UNCMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:UNCMA_22460
ORF Names:RCIX513
OrganismUncultured methanogenic archaeon RC-I [Complete proteome] [HAMAP]
Taxonomic identifier351160 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanocellalesMethanocellaceaeMethanocella

Protein attributes

Sequence length433 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 433433Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_1000018831

Regions

Domain111 – 317207ATP-grasp
Nucleotide binding138 – 19457ATP By similarity

Sites

Metal binding2751Magnesium or manganese 1 By similarity
Metal binding2871Magnesium or manganese 1 By similarity
Metal binding2871Magnesium or manganese 2 By similarity
Metal binding2891Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0W6Q4 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 6F81334A4362B0A7

FASTA43348,138
        10         20         30         40         50         60 
MKVLVIGSGG REHAIVEALA RSEYHPKIYA VMGNANPGIR RRAEDYLLEK ETNVPAIVQY 

        70         80         90        100        110        120 
AQDCNVDMAI IGPESPLAAG LADELELNGI PVVGPRKDAA RIEFDKAWTR EFMARNNIKG 

       130        140        150        160        170        180 
LPKFKVYDDY DDACRYLEDN PDVVVKPAGL TGGKGVKVMG EHMHTLEEAR EYVKSVLEHD 

       190        200        210        220        230        240 
RVVIEERLKG EEVTIMAFVD GKHVAPMPTV QDHKRAYEDD QGPNTGGMGS YTDNIDLLPF 

       250        260        270        280        290        300 
MTLDDYNEGV AIMEQTVKAM EKDVGVPYKG VLYGQFMITR DGMKVVEFNA RFGDPEAMNV 

       310        320        330        340        350        360 
LSLLKTDFVD ICEAIVDGSL DKLKIEFERS ATVCKYVVPA GYPDNPVKDA PLTVVEKPEY 

       370        380        390        400        410        420 
LVYYASVNER DGKVYTTSSR SLAIVGVADT IADAEILSEE GLSNVQGEFH CRHDIGKERL 

       430 
IRKRIEHMDA IRG 

« Hide

References

[1]"Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere."
Erkel C., Kube M., Reinhardt R., Liesack W.
Science 313:370-372(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM114193 Genomic DNA. Translation: CAJ35939.1.
RefSeqYP_685265.1. NC_009464.1.

3D structure databases

ProteinModelPortalQ0W6Q4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351160.RCIX513.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ35939; CAJ35939; RCIX513.
GeneID5144567.
KEGGrci:RCIX513.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMAYKGFLYA.

Enzyme and pathway databases

BioCycUMET351160:GJT4-2306-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_UNCMA
AccessionPrimary (citable) accession number: Q0W6Q4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways