Q0W6F7 (RIFK_UNCMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	UNCMA_21500
ORF Names:	RCIX631

Organism

Uncultured methanogenic archaeon RC-I [Complete proteome] [HAMAP]

Taxonomic identifier

351160 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › environmental samples

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	Riboflavin kinase HAMAP MF_01285		PRO_0000322109
Regions
	Nucleotide binding	101 – 106	6	CDP By similarity
	Nucleotide binding	195 – 198	4	CDP By similarity
	Region	1 – 91	91	H-T-H motif-like HAMAP MF_01285
	Region	92 – 214	123	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	130	1	Magnesium By similarity
	Metal binding	132	1	Magnesium By similarity
	Binding site	182	1	FMN By similarity
	Binding site	190	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0W6F7 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 6D9DFF5B68966F05	FASTA	214	23,670
	10 20 30 40 50 60 MRSIMEVETL KRLALMGANK EQVSLSSSIF ATSLGMSPQT AARRLSALEE DGYITRVVTP 70 80 90 100 110 120 EGQKVRITEK GITCLKSEYR DYCSIFEDGG APVMRGKVVT GLGEGQYYIS LDGYRNQFND 130 140 150 160 170 180 KLGFDPYPGT LNVRLTEPFI PAEHEAVVIA GFKGENRTFG GCKCYPVRIK GVRAAIIRPD 190 200 210 RTSYPPNLIE IIAPIKLRES LGLRDGDEVE VTLE

References

[1]	"Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere." Erkel C., Kube M., Reinhardt R., Liesack W. Science 313:370-372(2006) [PubMed: 16857943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM114193 Genomic DNA. Translation: CAJ36036.1.
RefSeq	YP_685362.1. NC_009464.1.
3D structure databases
ProteinModelPortal	Q0W6F7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0W6F7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5144087.
GenomeReviews	Gene locus UNCMA_21500 in contig AM114193_GR.
KEGG	rci:RCIX631.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10832.
HOGENOM	HBG553349.
OMA	GRHYISL.
ProtClustDB	CLSK2789654.
Enzyme and pathway databases
BioCyc	UMET351160:RCIX631-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_UNCMA

Accession

Primary (citable) accession number: Q0W6F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	September 5, 2006
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program