Q0W5U6 (DP2L_UNCMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit
Short name=Pol II
EC=2.7.7.7

Gene names

Name:	polC
Synonyms:	pol2b
Ordered Locus Names:	UNCMA_19460
ORF Names:	RCIX896

Organism

Uncultured methanogenic archaeon RC-I [Complete proteome] [HAMAP]

Taxonomic identifier

351160 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › environmental samples

Protein attributes

Sequence length	1134 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1134	1134	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000294700

Sequences

	Sequence		Length	Mass (Da)
	Q0W5U6 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: E6B275750F34B3D5	FASTA	1,134	126,003
	10 20 30 40 50 60 MPPKTSEAQE AYFKKLEAEF LHCREIATAA RRKGYDPSLE VEIPSATDLA DRVEVIMGVP 70 80 90 100 110 120 GLAAHIRKCE EKMSREEASL QVAADIAEGL VGKFKDEEEA VQCAVRTAVA VLTEGVVAAP 130 140 150 160 170 180 LEGISAVKLA KNDDGTEYIK VYFAGPIRSA GGTAEALAVL AADYVRRKTG RAPYKIRDVE 190 200 210 220 230 240 VERFVEEIML YKSIAHLQYT PTDEEIRLIV RNCPVCIDGE PTEQEEVQGY RNLERVETNR 250 260 270 280 290 300 VRGGIALVIA EGIILKAPKV KKHVDKLKFD GWEWLDKIIA GSKPAGGENK EEEKKIKPKD 310 320 330 340 350 360 KFLADLIAGR PVFGHPSRAG GFRLRYGRSR NTGFATAGIH PASMTIMDDF IATGTQLKVE 370 380 390 400 410 420 RPGKAAAMVP VDSLEGPTVR LFNGDVVRIS DEKTALKVRP DVSQILDNGE IIINYGDFLE 430 440 450 460 470 480 NNHTFVPSPY VEEWWIQDLE EKTKDKVEVN SPEEAFAVSE KYGVPLHPKY TYMWMDLTTD 490 500 510 520 530 540 DVVYLARYIS ASGMVENGEL RLPVEQRSKS LLEILLIPQK VRDNTVILSA EDTYILCRCL 550 560 570 580 590 600 GLNPDLSMKN PDAYAGLDSH AWKAVSALCG VTVMDRAPAR IGARMGRPEK SKLREMKPPV 610 620 630 640 650 660 HVLFPVGEAG GMRRSLQDAS AYSKSMTDRI GEIEVEVGRR KCPDCGKMTY MVACECGGHT 670 680 690 700 710 720 IPVYGCPDCG ISGIEGDCPK CHKPTTPNVK QKIDVKGLYA AALKRVGERD NFEVLKGVQG 730 740 750 760 770 780 LISKEKTPEP LEKGILRAKH EVFVFKDGTI RYDMSDVPLT HFIPREIGLS VEKARELGYE 790 800 810 820 830 840 KDTYGAPLES PEQVCELRVQ DIILSHDASN YLLKVCAFLD DELEKYYGLP RYYNVHEEQD 850 860 870 880 890 900 LIGHLVIGLA PHTSAGVLGR IIGFVSSSAG YAHPFFHAAK RRNCDGDEDC VMMLMDGLLN 910 920 930 940 950 960 FSLSYLPDRR GGKMDAPLVL SMRIDPKEID KESHNIDVMA RYPKEFYLAT REFKAPKDVE 970 980 990 1000 1010 1020 KIMDLVSKRL GTPEQYEGFK FTHGTSDIAA GPANSAYKTL GSMEDKLKAQ LELGRRLRAV 1030 1040 1050 1060 1070 1080 DEKDVAERVI NSHFLPDLIG NLRAFSTQQM RCVKCGERYR RPPLTGTCPR CGGGRVILTV 1090 1100 1110 1120 1130 HEGAVTKYMD VSLAIAKEYG VPSYTIQRLE LLSLSIKSLF ENDKSKQTGL ADFM

References

[1]	"Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere." Erkel C., Kube M., Reinhardt R., Liesack W. Science 313:370-372(2006) [PubMed: 16857943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM114193 Genomic DNA. Translation: CAJ36247.1.
RefSeq	YP_685573.1. NC_009464.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0W5U6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5142819.
GenomeReviews	Gene locus UNCMA_19460 in contig AM114193_GR.
KEGG	rci:RCIX896.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04008.
HOGENOM	HBG497074.
OMA	NCDGDED.
ProtClustDB	PRK04023.
Enzyme and pathway databases
BioCyc	UMET351160:RCIX896-MONOMER.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
KO	K02322.
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. PolC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_UNCMA

Accession

Primary (citable) accession number: Q0W5U6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	September 5, 2006
Last modified:	November 16, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program