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Q0W5A8 (AMPPA_UNCMA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
AMP phosphorylase

Short name=AMPpase
EC=2.4.2.-
Alternative name(s):
Nucleoside monophosphate phosphorylase
Short name=NMP phosphorylase
Gene names
Ordered Locus Names:UNCMA_17660
ORF Names:RCIX1126
OrganismUncultured methanogenic archaeon RC-I [Complete proteome] [HAMAP]
Taxonomic identifier351160 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanocellalesMethanocellaceaeMethanocella

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of AMP and phosphate to adenine and ribose 1,5-bisphosphate (R15P). Exhibits phosphorylase activity toward CMP and UMP in addition to AMP. Functions in an archaeal AMP degradation pathway, together with R15P isomerase and RubisCO By similarity. HAMAP-Rule MF_02132

Catalytic activity

AMP + phosphate = D-ribose 1,5-bisphosphate + adenine. HAMAP-Rule MF_02132

CMP + phosphate = D-ribose 1,5-bisphosphate + cytosine. HAMAP-Rule MF_02132

UMP + phosphate = D-ribose 1,5-bisphosphate + uracile. HAMAP-Rule MF_02132

Sequence similarities

Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503AMP phosphorylase HAMAP-Rule MF_02132
PRO_0000314733

Regions

Nucleotide binding194 – 1996AMP By similarity

Sites

Active site2561Proton donor By similarity
Binding site1681AMP; via amide nitrogen By similarity
Binding site2031AMP; via amide nitrogen By similarity
Binding site2641AMP By similarity
Binding site2881AMP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0W5A8 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: FA8144704978BBBC

FASTA50354,495
        10         20         30         40         50         60 
MKLRAQPYDI EVGRYEVIIN SADAEELGVL AGDRVQVKDK DTITAVVETT DAIVSPGKIG 

        70         80         90        100        110        120 
IYREAWESIK VVPDEVVEVL PAAKPKSVSF IRKKMDKQKL TSEEMHAIIE DIVDGNLTEV 

       130        140        150        160        170        180 
ELTAFVTASY IYTMDSDEIE WMTRAMVKTG DQISFDVHPV MDHHSIGGVP GNKISLCIVP 

       190        200        210        220        230        240 
IIAAAGLLIP KTSSRAITGA GGSADLMEIL CPVSFRADEI KKMTTKVGGC LVWGGATNIA 

       250        260        270        280        290        300 
PADDKIINVE YPLSIDPKSQ LLASVMAKKF AVGADTMVLD IPCGNETKIP TVQEGRKLAR 

       310        320        330        340        350        360 
DFMELGDRLG MKIECALTYG GTPLGRAIGG GVEVREAMVM LEKFEGPRSL LEKTIAISGM 

       370        380        390        400        410        420 
MLEMGGVAPK NEGAKMAVEL VKSGKALQKF KEIIEVQGGD PKVTSDMVPI GDKVATVLSP 

       430        440        450        460        470        480 
QDGYVLEISN KRLVYMCRLA GAPHDKGVGV ILHKKKGEYV KKGDGLFTLY ADKEWKLDAA 

       490        500 
IKESLRNPIM MVEGMILEKI EVV 

« Hide

References

[1]"Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere."
Erkel C., Kube M., Reinhardt R., Liesack W.
Science 313:370-372(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM114193 Genomic DNA. Translation: CAJ36435.1.
RefSeqYP_685761.1. NC_009464.1.

3D structure databases

ProteinModelPortalQ0W5A8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351160.RCIX1126.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ36435; CAJ36435; RCIX1126.
GeneID5144338.
KEGGrci:RCIX1126.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0213.
HOGENOMHOG000252767.
KOK00758.
OMAESVPGFH.
ProtClustDBPRK04350.

Enzyme and pathway databases

BioCycUMET351160:GJT4-1811-MONOMER.

Family and domain databases

Gene3D3.40.1030.10. 1 hit.
3.90.1170.30. 1 hit.
HAMAPMF_02132. AMP_phosphorylase.
InterProIPR017713. AMP_phosphorylase.
IPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR013102. PYNP_C.
IPR000053. Pyrmidine_PPase.
IPR013466. Thymidine/AMP_Pase.
[Graphical view]
PANTHERPTHR10515. PTHR10515. 1 hit.
PfamPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
PIRSFPIRSF000478. TP_PyNP. 1 hit.
SMARTSM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMSSF47648. SSF47648. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsTIGR03327. AMP_phos. 1 hit.
TIGR02645. ARCH_P_rylase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAMPPA_UNCMA
AccessionPrimary (citable) accession number: Q0W5A8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: February 19, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families