Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q0W3T3 (Q0W3T3_UNCMA) Unreviewed, UniProtKB/TrEMBL

Last modified November 16, 2011. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Adenylosuccinate synthetase HAMAP MF_00011
Short name=AMPSase HAMAP MF_00011
Short name=AdSS HAMAP MF_00011
EC=6.3.4.4 HAMAP MF_00011
Alternative name(s):
IMP--aspartate ligase HAMAP MF_00011
Gene names
Name:purA HAMAP MF_00011 EMBL CAJ36960.1
Ordered Locus Names:UNCMA_12780
ORF Names:RCIX1751 EMBL CAJ36960.1
OrganismUncultured methanogenic archaeon RC-I [Complete proteome] [HAMAP] EMBL CAJ36960.1
Taxonomic identifier351160 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › environmental samples

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays an important role in the de novo pathway of purine nucleotide biosynthesis By similarity. RuleBase RU000520

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011

Catalytic activity

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. RuleBase RU000520 HAMAP MF_00011

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. RuleBase RU000520 HAMAP MF_00011

Subunit structure

Homodimer By similarity. HAMAP MF_00011

Subcellular location

Cytoplasm By similarity HAMAP MF_00011.

Sequence similarities

Belongs to the adenylosuccinate synthetase family. HAMAP MF_00011 RuleBase RU004163

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding12 – 187GTP By similarity HAMAP MF_00011
Nucleotide binding42 – 443GTP By similarity HAMAP MF_00011
Nucleotide binding281 – 2833GTP By similarity HAMAP MF_00011
Nucleotide binding321 – 3233GTP By similarity HAMAP MF_00011
Region13 – 164IMP binding By similarity HAMAP MF_00011
Region40 – 434IMP binding By similarity HAMAP MF_00011
Region249 – 2557Substrate binding By similarity HAMAP MF_00011

Sites

Active site131Proton acceptor By similarity HAMAP MF_00011
Active site431Proton donor By similarity HAMAP MF_00011
Metal binding131Magnesium By similarity HAMAP MF_00011
Metal binding421Magnesium; via carbonyl oxygen By similarity HAMAP MF_00011
Binding site1241IMP By similarity HAMAP MF_00011
Binding site1381IMP; shared with dimeric partner By similarity HAMAP MF_00011
Binding site1761IMP By similarity HAMAP MF_00011
Binding site1911IMP By similarity HAMAP MF_00011
Binding site2531IMP By similarity HAMAP MF_00011
Binding site2551GTP By similarity HAMAP MF_00011

Sequences

Sequence LengthMass (Da)Tools
Q0W3T3 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 890A269F2C47DC86

FASTA33536,024
        10         20         30         40         50         60 
MVTTIVVGGF FGDEGKGKIV AHIAYTDHPS IIARGGVGPN AGHTVIKDGV KHGVRMIPSG 

        70         80         90        100        110        120 
FIYDNARLLI GAGVLVDPVV LEREVALLNA GNRLGVDYRC SIIEAKHIEE DKGTERLAKK 

       130        140        150        160        170        180 
IGTTGTGCGP ANKERVMRSA LQAKDVPALK SYLTDVAKEC NDAIDRGESI IIEGSQGFGI 

       190        200        210        220        230        240 
SLYYGTYPFV TSKDTSASQL AADVGVGPTK IDEVVVVFKA FPTRVGEGPF PTEMTREDAQ 

       250        260        270        280        290        300 
KRGIAEFGTV TGRARRIGYW DPEMARYSAM INGATQAAIT GLDRIDPACK GVTDWDRLPD 

       310        320        330 
KVKDFIRQAE KDVGVPFTLL STGPSLDEII DLRKR

« Hide

References

[1]"Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere."
Erkel C., Kube M., Reinhardt R., Liesack W.
Science 313:370-372(2006) [PubMed: 16857943] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM114193 Genomic DNA. Translation: CAJ36960.1.
RefSeqYP_686286.1. NC_009464.1.

3D structure databases

ProteinModelPortalQ0W3T3.
SMRQ0W3T3. Positions 1-333.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0W3T3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5143947.
GenomeReviewsGene locus UNCMA_12780 in contig AM114193_GR.
KEGGrci:RCIX1751.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGarNOG04146.
HOGENOMHBG658237.
OMAIDPHVGI.
ProtClustDBPRK04293.

Enzyme and pathway databases

BioCycUMET351160:RCIX1751-MONOMER.

Family and domain databases

HAMAPMF_00011. Adenylosucc_synth.
[Tree]
InterProIPR018220. Adenylosuccinate_synthase_AS.
IPR001114. Adenylosuccinate_synthetase.
[Graphical view]
KOK01939.
PANTHERPTHR11846. Asucc_synthtase. 1 hit.
PfamPF00709. Adenylsucc_synt. 2 hits.
[Graphical view]
SMARTSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
PROSITEPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ0W3T3_UNCMA
AccessionPrimary (citable) accession number: Q0W3T3
Entry history
Integrated into UniProtKB/TrEMBL: September 5, 2006
Last sequence update: September 5, 2006
Last modified: November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info