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Q0W3B6 (DAPF_UNCMA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diaminopimelate epimerase

Short name=DAP epimerase
EC=5.1.1.7
Gene names
Name:dapF
Ordered Locus Names:UNCMA_11190
ORF Names:RCIX1964
OrganismUncultured methanogenic archaeon RC-I [Complete proteome] [HAMAP]
Taxonomic identifier351160 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanocellalesMethanocellaceaeMethanocella

Protein attributes

Sequence length280 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine By similarity. HAMAP-Rule MF_00197

Catalytic activity

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP-Rule MF_00197

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. HAMAP-Rule MF_00197

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00197.

Sequence similarities

Belongs to the diaminopimelate epimerase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   Molecular functionIsomerase
   PTMDisulfide bond
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlysine biosynthetic process via diaminopimelate

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondiaminopimelate epimerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 280280Diaminopimelate epimerase HAMAP-Rule MF_00197
PRO_1000011979

Regions

Region76 – 783Substrate binding By similarity
Region210 – 2112Substrate binding By similarity
Region221 – 2222Substrate binding By similarity

Sites

Active site761Proton donor/acceptor By similarity
Active site2201Proton donor/acceptor By similarity
Binding site141Substrate By similarity
Binding site491Substrate By similarity
Binding site671Substrate By similarity
Binding site1931Substrate By similarity
Site1611Important for catalytic activity By similarity
Site2101Important for catalytic activity By similarity

Amino acid modifications

Disulfide bond76 ↔ 220 HAMAP-Rule MF_00197

Sequences

Sequence LengthMass (Da)Tools
Q0W3B6 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 3DE0C9B952CFC360

FASTA28030,722
        10         20         30         40         50         60 
MTKIKFTKMH GNGNDFIVID EFENPVPEEK KAAFAKKVCH RRFGIGADGV LFLAKPLHTS 

        70         80         90        100        110        120 
LHMRIFNEDG SEAEMCGNGI RCFVKYAVDN GHMNPGKDKV ETKAGILEVE ARIEDGKTLV 

       130        140        150        160        170        180 
KVSMGKPLFD PKKIPAAGLN NFINKPLHGY EVTAVNTGVP HAVIFVDDVN AVDLMKVAPE 

       190        200        210        220        230        240 
IRYDLKTFPK GINVNFVQRE GHNLRVRTYE RGVEGETLSC GTGSVASAAV ARYLGYTRDE 

       250        260        270        280 
TTVYTAGGQL NISFVSDIAY MEGPAETVYE GEIDVDFSAL 

« Hide

References

[1]"Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere."
Erkel C., Kube M., Reinhardt R., Liesack W.
Science 313:370-372(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM114193 Genomic DNA. Translation: CAJ37127.1.
RefSeqYP_686453.1. NC_009464.1.

3D structure databases

ProteinModelPortalQ0W3B6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351160.RCIX1964.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ37127; CAJ37127; RCIX1964.
GeneID5144870.
KEGGrci:RCIX1964.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0253.
HOGENOMHOG000220466.
KOK01778.
OMALIVEPPY.

Enzyme and pathway databases

BioCycUMET351160:GJT4-1146-MONOMER.
UniPathwayUPA00034; UER00025.

Family and domain databases

HAMAPMF_00197. DAP_epimerase.
InterProIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERPTHR31689. PTHR31689. 1 hit.
PfamPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsTIGR00652. DapF. 1 hit.
PROSITEPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPF_UNCMA
AccessionPrimary (citable) accession number: Q0W3B6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: June 11, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways