Q0W2X4 (KHSE_UNCMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	UNCMA_09790
ORF Names:	RCIX2142

Organism

Uncultured methanogenic archaeon RC-I [Complete proteome] [HAMAP]

Taxonomic identifier

351160 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › environmental samples

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Homoserine kinase HAMAP MF_00384		PRO_1000049194
Regions
	Nucleotide binding	82 – 92	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0W2X4 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 04BA9B52EDB2C4B2	FASTA	300	31,169
	10 20 30 40 50 60 MDSITVKASA TSANLGAGFD VMGIALESPG DIIRVEKADE LSIVVKGRGA EGIPADPEKN 70 80 90 100 110 120 TAGLVALAMD KKVRITIKSG IRPGSGLGSS AAPAAGTAVA INELFSLGYS KEELVWIAAR 130 140 150 160 170 180 GEMAAAGIVH ADNVAPCIMG GLTLVCNNYV EHVELPPMGV VAVLPEIVVS TKSARGILPQ 190 200 210 220 230 240 QVPLQEMVLN VSKAAMLMAG VVKKDPVIIG RSLSDTFNEK YRSPLIKGYG SVREAALDSG 250 260 270 280 290 300 AYGVAISGSG PTMLALCPED RSFDVAAAMR KKFEEAGVVS EAYVTCIGKG VRIINRDEEE

References

[1]	"Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere." Erkel C., Kube M., Reinhardt R., Liesack W. Science 313:370-372(2006) [PubMed: 16857943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM114193 Genomic DNA. Translation: CAJ37269.1.
RefSeq	YP_686595.1. NC_009464.1.
3D structure databases
ProteinModelPortal	Q0W2X4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0W2X4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5143840.
GenomeReviews	Gene locus UNCMA_09790 in contig AM114193_GR.
KEGG	rci:RCIX2142.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05905.
HOGENOM	HBG646290.
OMA	IPGYDNA.
PhylomeDB	Q0W2X4.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	UMET351160:RCIX2142-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_UNCMA

Accession

Primary (citable) accession number: Q0W2X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program