Q0W2W0 (PIMT_UNCMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-L-isoaspartate O-methyltransferase
EC=2.1.1.77

Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Short name=PIMT

Gene names

Name:	pcm
Ordered Locus Names:	UNCMA_09660
ORF Names:	RCIX2158

Organism

Uncultured methanogenic archaeon RC-I [Complete proteome] [HAMAP]

Taxonomic identifier

351160 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanocellales › Methanocellaceae › Methanocella › Methanocella arvoryzae

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity. HAMAP-Rule MF_00090
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP-Rule MF_00090
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Protein-L-isoaspartate O-methyltransferase HAMAP-Rule MF_00090		PRO_0000351974
Sites
	Active site	33	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0W2W0 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: BF2A4C34ACE3DAE0	FASTA	188	20,391
	10 20 30 40 50 60 MDRVPREEFV PEDVRPDAYY DTPLPIGHNQ TISAPSMVAI MCQALDIREG NKVLEIGTGL 70 80 90 100 110 120 GYHAAVMAVL AGASGVVYTV ERIPELADMA RSVLSRLGFD NVKVFLRDGT EGLPDFAPYD 130 140 150 160 170 180 RISVAAAAPD VPQPLVDQLK DPGRLVIPVG RYFQQLMLVE KKGGQIITTD KGGVAFVPLL GKYGFKGY

References

[1]	"Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere." Erkel C., Kube M., Reinhardt R., Liesack W. Science 313:370-372(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM114193 Genomic DNA. Translation: CAJ37283.1.
RefSeq	YP_686609.1. NC_009464.1.
3D structure databases
ProteinModelPortal	Q0W2W0.
ModBase	Search...
Protein-protein interaction databases
STRING	351160.RCIX2158.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ37283; CAJ37283; RCIX2158.
GeneID	5143578.
KEGG	rci:RCIX2158.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2518.
HOGENOM	HOG000257189.
KO	K00573.
OMA	RILFSAC.
ProtClustDB	CLSK2789256.
Family and domain databases
HAMAP	MF_00090. PIMT. Divergent sequence.
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PTHR11579. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_UNCMA

Accession

Primary (citable) accession number: Q0W2W0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	September 5, 2006
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program