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Q0W150 (LIPA_UNCMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable lipoyl synthase

EC=2.8.1.8
Alternative name(s):
Lip-syn
Short name=LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene names
Name:lipA
Ordered Locus Names:UNCMA_03690
ORF Names:RRC130
OrganismUncultured methanogenic archaeon RC-I [Complete proteome] [HAMAP]
Taxonomic identifier351160 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanocellalesMethanocellaceaeMethanocella

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00206.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprotein lipoylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lipoate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 296296Probable lipoyl synthase HAMAP-Rule MF_00206
PRO_0000325325

Sites

Metal binding381Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding431Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding491Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding641Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding681Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding711Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0W150 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 2DDC3215EA6D491C

FASTA29633,008
        10         20         30         40         50         60 
MPDPTSPKPD WLKVRLPRTD KYGAVKDVIK KYNLNTVCSS AMCPNAFECW DGGCLTFMVL 

        70         80         90        100        110        120 
GNTCTRACRF CTVTHGPAGE PLDSNEPQRL AAAAKELDLS YVVITSVDRD DLPDYGAGHY 

       130        140        150        160        170        180 
AACIRAVKEQ LPGARVEAII PDFTGRLDLL EQVVDARPDV ISHNIETVER LSPSVRDRRA 

       190        200        210        220        230        240 
GYYRSLDVLR DVKRVNPHML TKSSLLLGMG EEDIEIKEAL HDLQEARVDI VTLGQYLRPS 

       250        260        270        280        290 
IRQWPVHRYV APGEFSELAE YGRSLGFKYV AAGPFVRTSY RAGEQYVSVI ADSRMA 

« Hide

References

[1]"Genome of rice cluster I archaea -- the key methane producers in the rice rhizosphere."
Erkel C., Kube M., Reinhardt R., Liesack W.
Science 313:370-372(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM114193 Genomic DNA. Translation: CAJ37893.1.
RefSeqYP_687219.1. NC_009464.1.

3D structure databases

ProteinModelPortalQ0W150.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351160.RRC130.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ37893; CAJ37893; RRC130.
GeneID5144186.
KEGGrci:RRC130.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0320.
HOGENOMHOG000235998.
KOK03644.
OMAPEEPYNT.

Enzyme and pathway databases

BioCycUMET351160:GJT4-383-MONOMER.
UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLIPA_UNCMA
AccessionPrimary (citable) accession number: Q0W150
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways