Reviewed, UniProtKB/Swiss-Prot Q0VT66 (RNPH_ALCBS)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	ABO_0206

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Ribonuclease PH HAMAP MF_00564		PRO_1000024769

Sequences

	Sequence		Length	Mass (Da)
	Q0VT66-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: A69CCB3802DA6C74	FASTA	238	25,617
	10 20 30 40 50 60 MRPSGRAPDQ LRELSFTRNY TVHAEGSVLV AFGNTKVLCT ASVEDGVPRF LKGKGQGWLT 70 80 90 100 110 120 AEYSMLPRST HTRSGREATR GKQGGRTLEI QRLIGRSLRA AVDMKALGER TIYLDCDVLQ 130 140 150 160 170 180 ADGGTRTASI SGACVALVDA FTYLLETKKI KNDPLTGLVG AVSVGMYKDT PVLDLDYAED 190 200 210 220 230 SNAGTDMNVV MTQQGGFIEI QGSAEGAPFT REQSDNLLEL AENGIGEIIK AQQVALGW

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286690 Genomic DNA. Translation: CAL15654.1.
RefSeq	YP_691926.1.
3D structure databases
SMR	Q0VT66. Positions 2-237.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VT66.
Genome annotation databases
GeneID	4212173.
GenomeReviews	Gene locus ABO_0206 in contig AM286690_GR.
KEGG	abo:ABO_0206.
NMPDR	fig|393595.12.peg.205.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
HOGENOM	HBG737187.
OMA	MLPRATG.
PhylomeDB	Q0VT66.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0206-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_ALCBS

Accession

Primary (citable) accession number: Q0VT66

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)