Reviewed, UniProtKB/Swiss-Prot Q0VT62 (ARGB_ALCBS)

Names and origin

Protein names

Recommended name:
    Acetylglutamate kinase
    EC=2.7.2.8
Alternative name(s):
    NAG kinase
      Short name=AGK
    N-acetyl-L-glutamate 5-phosphotransferase

Gene names

Name:	argB
Ordered Locus Names:	ABO_0210

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082
Subcellular location	Cytoplasm Probable HAMAP MF_00082.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP acetylglutamate kinase activity Inferred from electronic annotation. Source: HAMAP glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Acetylglutamate kinase HAMAP MF_00082		PRO_0000264678
Regions
	Region	66 – 67	2	Substrate binding By similarity
Sites
	Binding site	88	1	Substrate By similarity
	Binding site	196	1	Substrate By similarity
	Site	31	1	Transition state stabilizer By similarity
	Site	256	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VT62-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 21A81BA3866981C8	FASTA	299	31,829
	10 20 30 40 50 60 MDANSAHDTA RILIEALPYI QRFAGTTVVI KYGGNAMENE ELKNSFARDV VMMKQVGIHP 70 80 90 100 110 120 VIVHGGGPQI GELLERLGKE SKFVQGMRVT DRETMDVVQM VLGGLVNKDI VNLIQHNGGQ 130 140 150 160 170 180 AIGLTGKDGR LIKARKMVLK ASDADSPALQ ASEIIDIGHV GEVQGIDTRI IDLLAGSDFI 190 200 210 220 230 240 PVIAPIGVDD NGASYNINAD LVAGKVAEVL RAEKLILLTN VPGLKDKQGQ ILTGLTTERV 250 260 270 280 290 NSLIKDGTIY GGMLPKIGCA LEAVQNGVHT AHIIDGRVSH AVLLEILTDK GIGTLISKD

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286690 Genomic DNA. Translation: CAL15658.1.
RefSeq	YP_691930.1.
3D structure databases
SMR	Q0VT62. Positions 1-297.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VT62.
Genome annotation databases
GeneID	4213477.
GenomeReviews	Gene locus ABO_0210 in contig AM286690_GR.
KEGG	abo:ABO_0210.
NMPDR	fig|393595.12.peg.209.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG497643.
OMA	MDIVEMV.
PhylomeDB	Q0VT62.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0210-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR011148. GlcNAc_kinase. IPR001057. Glu_5kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR00761. argB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_ALCBS

Accession

Primary (citable) accession number: Q0VT62

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	September 5, 2006
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)