Q0VSY7 (GSH1_ALCBS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--cysteine ligase
EC=6.3.2.2

Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase

Gene names

Name:	gshA
Ordered Locus Names:	ABO_0263

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Glutamate--cysteine ligase HAMAP MF_00578		PRO_1000025167

Sequences

	Sequence		Length	Mass (Da)
	Q0VSY7 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 0C5DF416630C2313	FASTA	525	59,733
	10 20 30 40 50 60 MKHLEQRIHA LLNADAAATL AGIRRGIEKE SLRITTDGTL AQTPHPRALG SALTHPYITT 70 80 90 100 110 120 DYSEALLEFI TPPSTELHKP IEFLEQLHRY VYSHIGDEVL WVNSMPCMIG KDSDVPVAQY 130 140 150 160 170 180 GSSNVGRMKT VYREGLGHRY GRKMQTIAGI HYNFSYPDAF WKLNQQLEGD NAPLQDYISR 190 200 210 220 230 240 RYFDLTRNFQ RYSWLLVYLF GASPALCASF LAGREHDLLE RFDHSLYRPQ ATSLRMSDLG 250 260 270 280 290 300 YQNNAQSSLA ISYNNLDEYV STLTHAINTP EPAYEKMGVK VTDADGNVKY QQLNANILQI 310 320 330 340 350 360 ENEYYSSIRP KRTIKPGERP TTALQERGVE YIEIRALDLN PFEPVGINQQ EIRFLDLFAT 370 380 390 400 410 420 YCLLRESPQL EKCDLHASKE NLRKVVYDGR NTDIQLYNWG KSVSLKNWAS EKLTQMQPIA 430 440 450 460 470 480 ELFDRAHGGN NYAEALAHQQ EKVDNPSATP SAQILEQLES RNQGFFQFAM EQALAHRDHF 490 500 510 520 LSGQRCDATN QQLEALAADS LAEQAAGEAA DQQSFEEYLA DYFHD

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SK2 / ATCC 700651 / DSM 11573.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286690 Genomic DNA. Translation: CAL15711.1.
RefSeq	YP_691983.1. NC_008260.1.
3D structure databases
ProteinModelPortal	Q0VSY7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VSY7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4212782.
GenomeReviews	Gene locus ABO_0263 in contig AM286690_GR.
KEGG	abo:ABO_0263.
NMPDR	fig|393595.12.peg.260.
PATRIC	20838125. VBIAlcBor124741_0280.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2918.
HOGENOM	HBG289023.
OMA	ADTLYLP.
PhylomeDB	Q0VSY7.
ProtClustDB	CLSK2779225.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0263-MONOMER.
Family and domain databases
HAMAP	MF_00578. Glu_cys_ligase. [Tree]
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
KO	K01919.
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. Glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_ALCBS

Accession

Primary (citable) accession number: Q0VSY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program