Reviewed, UniProtKB/Swiss-Prot Q0VSS5 (TPIS_ALCBS)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase
      Short name=TIM
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	ABO_0325

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	Triosephosphate isomerase HAMAP MF_00147		PRO_0000307421
Sites
	Active site	92	1	Electrophile By similarity
	Active site	164	1	Proton acceptor By similarity
	Binding site	10	1	Substrate By similarity
	Binding site	12	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VSS5-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 951CC6FA7F9A4DCD	FASTA	247	25,415
	10 20 30 40 50 60 MNRTPLVAGN WKMNGRKDLL TEMADALAGY AGAAEVVICP PFPYLAAARS AMPGAVGIGS 70 80 90 100 110 120 QNVSEQEDGA FTGEVSAPML AEVGCGYVII GHSERRALYG ETDAQIASKF AHAQAAGLVP 130 140 150 160 170 180 ILCVGETLEE RDSGNTDTIV CGQLRAVVDA VGVAAFANAI VAYEPVWAIG TGKTASPEQA 190 200 210 220 230 240 QAVHACLRQF LAKDDPDIAQ SLRLLYGGSV KADNAALLFA QSDIDGGLIG GASLTADSFI AICQAAE

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL15773.1.
RefSeq	YP_692045.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4211921.
GenomeReviews	Gene locus ABO_0325 in contig AM286690_GR.
KEGG	abo:ABO_0325.
NMPDR	fig|393595.12.peg.324.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VSS5.
OMA	Q0VSS5. IGAQDCH.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0325-MON.
Family and domain databases
HAMAP	MF_00147. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
ProDom	PD001005. Triophos_ismrse. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_ALCBS

Accession

Primary (citable) accession number: Q0VSS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)