Reviewed, UniProtKB/Swiss-Prot Q0VSR7 (PNP_ALCBS)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide nucleotidyltransferase
    EC=2.7.7.8
Alternative name(s):
    Polynucleotide phosphorylase
      Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	ABO_0333

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	697 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 697	697	Polyribonucleotide nucleotidyltransferase HAMAP MF_01595		PRO_0000329489
Regions
	Domain	555 – 614	60	KH
	Domain	624 – 692	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	Q0VSR7-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 29A4A86B27D2FD6F	FASTA	697	75,304
	10 20 30 40 50 60 MFNKITKQIQ FGRDTVTLET GQIARQATAA VMVRIGDTQV LVTVVGRKEA NPGQNFFPLT 70 80 90 100 110 120 VNYQEKTYAT GRIPGGFLKR EGRPSEKETL TCRLIDRPIR PLFPKGFMNE VQVVATVMSS 130 140 150 160 170 180 DKNRDPDIAA LIGTSAALSI SGIPFSGPIG AARVGYKDGM YILNPSYSEL AESALDLVVA 190 200 210 220 230 240 GTEPAVLMVE SEAQELSEDQ MLGAVLFGHM EMQPLIQGIK EFAAEVGTET WDWKPAEQNE 250 260 270 280 290 300 TLKAAIKDKF AAALGEAYTI TEKMARYAKV GELRDACVAE FATGEDGAPE ADEVKDLFGK 310 320 330 340 350 360 IEKSVVREAV VSGKPRIDGR ALDAVRAIDC QVGTLAKTHG SALFTRGETQ AIVTATLGGM 370 380 390 400 410 420 RDAQFIDALE GSHQDHFMLQ YNFPPYCVGE TGFIGSPKRR EIGHGRLARR GVEAVVPSVQ 430 440 450 460 470 480 DFPYTIRVVS EITESNGSSS MASVCGTSMA LMDAGVPLTA PVAGIAMGLV KEEDGRYAVL 490 500 510 520 530 540 SDILGDEDHL GDMDFKVAGT ARGVTALQMD IKIEGITEEI MEKALNQANA GRLHILGEMN 550 560 570 580 590 600 KAIAESRSEV SDNAPTLLTL KINPDKIRDV IGKGGATIRA LTEETGCTID IEDDGSVKIY 610 620 630 640 650 660 GETREKADEA VRRVEEITAE AEVGAIYEGK VTRVVDFGAF VAIMPGTEGL LHISQIAEER 670 680 690 VEKVTDYVNE GEIIKVKVLD VDQRGRIKLS MKEAKED

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL15781.1.
RefSeq	YP_692053.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4212265.
GenomeReviews	Gene locus ABO_0333 in contig AM286690_GR.
KEGG	abo:ABO_0333.
NMPDR	fig|393595.12.peg.333.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VSR7.
OMA	Q0VSR7. GHGNLAK.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0333-MON.
Family and domain databases
HAMAP	MF_01595. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
PANTHER	PTHR11252. PNPase. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03591. Polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_ALCBS

Accession

Primary (citable) accession number: Q0VSR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)