Reviewed, UniProtKB/Swiss-Prot Q0VSE5 (PROB_ALCBS)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	ABO_0455

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Glutamate 5-kinase HAMAP MF_00456		PRO_0000252969
Regions
	Domain	275 – 353	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q0VSE5-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 027823FD5651C897	FASTA	376	40,277
	10 20 30 40 50 60 MADRQRWVIK IGSALLTNDG KGLDCALMQT WVDRMVALRE RGIEVVIVSS GAVAEGMTRL 70 80 90 100 110 120 GWSARPDSVH ELQAAAAVGQ MGLVQAWETR FQQHQLHTAQ VLLTHDDLSD RKRYLNARST 130 140 150 160 170 180 LLTLLGFGVV PVVNENDTVV TDEIRFGDND TLAALVANLV EADRLLILTD QEGLFDADPR 190 200 210 220 230 240 SNADANLIRE ARASDPAIVR VAGGGGLLGR GGMATKVRAG KLAARSGAIT TIASGRSPEV 250 260 270 280 290 300 LIELADGQGP GTTLLPDTTP MNARKQWLAG HLQMRGRLVL DAGAVVRLRE GGSSLLPVGV 310 320 330 340 350 360 VDVFGQFSRG EMVACEDERG ERVGCGLVNY DAADARRICR KKSAEIAGVL GFMNEEELIH 370 RDNLVLMEAG SRVSGA

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL15903.1.
RefSeq	YP_692175.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VSE5.
Genome annotation databases
GeneID	4211220.
GenomeReviews	Gene locus ABO_0455 in contig AM286690_GR.
KEGG	abo:ABO_0455.
NMPDR	fig|393595.12.peg.454.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VSE5.
OMA	ILTDQRG.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0455-MON.
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_ALCBS

Accession

Primary (citable) accession number: Q0VSE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	September 5, 2006
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)