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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei561Aminoacyl-adenylateUniRule annotation1
Binding sitei605ATPUniRule annotation1
Metal bindingi900ZincUniRule annotation1
Metal bindingi903ZincUniRule annotation1
Metal bindingi920ZincUniRule annotation1
Metal bindingi923ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciABOR393595:G1G1O-477-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:ABO_0459
OrganismiAlcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)
Taxonomic identifieri393595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesAlcanivoracaceaeAlcanivorax
Proteomesi
  • UP000008871 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000220391 – 937Isoleucine--tRNA ligaseAdd BLAST937

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi393595.ABO_0459

Structurei

3D structure databases

ProteinModelPortaliQ0VSE1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi602 – 606"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q0VSE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYKATLNL PQTGFPMKAG LSQREPARLK EWQQKQLYQK IREAFAGRPK
60 70 80 90 100
FILHDGPPYA NGDIHIGHAV NKILKDMIVK SRTLAGFDAP YVPGWDCHGL
110 120 130 140 150
PIELMVEKKV GKAGHKVDAG TFRKKCREYA SKQVAGQKSD FMRLGVLGDW
160 170 180 190 200
DNPYLTMDFT FEANIIRSLG KIVDNGHLQQ GFKPVHWCLD CGSALAEAEV
210 220 230 240 250
EYEDKISPAI DVAFPVVDVA DFVARSGIEA SAPALVIWTT TPWTLPANRA
260 270 280 290 300
VAVHPELDYV LLSGELSGVA RELLVAEALA DDLVTRWGLE NVTRSAAVAG
310 320 330 340 350
SKLEMLALQH PFLEAQVPVV FGEHVTTDAG TGLVHTAPGH GVDDFMVGKQ
360 370 380 390 400
YDLDPISPVL DNGLFREDLP VVGGLHVSKA NEPVIEALKD SGNLVKLAKI
410 420 430 440 450
EHSYPHCWRH KTPLIFRATA QWFVSMDQAG LLPRARQEID KVQWLPEWGK
460 470 480 490 500
ARIEGMLTDR PDWCISRQRT WGVPIALFVN KETSELHPQT PALIEQVAQR
510 520 530 540 550
VEKAGVDAWF DLDPAELLGD EADQYSKVTD TLDVWFDSGV THYCVLDQRE
560 570 580 590 600
QLRAPADLYL EGSDQHRGWF QSSLLTSLAI RDAAPYSTVL THGFTVDEHG
610 620 630 640 650
RKMSKSVGNV IAPQEVWNDL GADILRLWVC ATDYRGEMSV SKDILKQMGD
660 670 680 690 700
SYRRIRNTSR FLLSNLSGFE PATDALQPEQ MLALDRYIVD RALQVQAEIQ
710 720 730 740 750
DLYDGYHFHQ VYQKLHNFCA LDLGGFYLDI IKDRQYTTQA DSVARRSCQT
760 770 780 790 800
ALYHIAQALV RWMAPVLSFT AEEIYENLPG ERLDSVFLAE WYDGLFALAD
810 820 830 840 850
NADMGRAFWD KVQDAKQAVN KAIEGARAAK LIKGSLSAEV VLFVDAEQNA
860 870 880 890 900
LLQRLGDELR FVTITSAAVL KPLAEAPAEL EDTSVAGLKV QVLASDHAKC
910 920 930
ARCWHHQPDV GSHAEHPELC GRCITNVEGD GEVRHYA
Length:937
Mass (Da):104,314
Last modified:September 5, 2006 - v1
Checksum:iDB713DE4653A6E4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286690 Genomic DNA Translation: CAL15907.1
RefSeqiWP_011587745.1, NC_008260.1

Genome annotation databases

EnsemblBacteriaiCAL15907; CAL15907; ABO_0459
KEGGiabo:ABO_0459

Similar proteinsi

Entry informationi

Entry nameiSYI_ALCBS
AccessioniPrimary (citable) accession number: Q0VSE1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health