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Q0VSE1

- SYI_ALCBS

UniProt

Q0VSE1 - SYI_ALCBS

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Protein

Isoleucine--tRNA ligase

Gene
ileS, ABO_0459
Organism
Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylate By similarity
Binding sitei605 – 6051ATP By similarity
Metal bindingi900 – 9001Zinc By similarity
Metal bindingi903 – 9031Zinc By similarity
Metal bindingi920 – 9201Zinc By similarity
Metal bindingi923 – 9231Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciABOR393595:GHRI-471-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:ABO_0459
OrganismiAlcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573)
Taxonomic identifieri393595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesAlcanivoracaceaeAlcanivorax
ProteomesiUP000008871: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 937937Isoleucine--tRNA ligaseUniRule annotationPRO_1000022039Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi393595.ABO_0459.

Structurei

3D structure databases

ProteinModelPortaliQ0VSE1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi602 – 6065"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0VSE1-1 [UniParc]FASTAAdd to Basket

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MTDYKATLNL PQTGFPMKAG LSQREPARLK EWQQKQLYQK IREAFAGRPK    50
FILHDGPPYA NGDIHIGHAV NKILKDMIVK SRTLAGFDAP YVPGWDCHGL 100
PIELMVEKKV GKAGHKVDAG TFRKKCREYA SKQVAGQKSD FMRLGVLGDW 150
DNPYLTMDFT FEANIIRSLG KIVDNGHLQQ GFKPVHWCLD CGSALAEAEV 200
EYEDKISPAI DVAFPVVDVA DFVARSGIEA SAPALVIWTT TPWTLPANRA 250
VAVHPELDYV LLSGELSGVA RELLVAEALA DDLVTRWGLE NVTRSAAVAG 300
SKLEMLALQH PFLEAQVPVV FGEHVTTDAG TGLVHTAPGH GVDDFMVGKQ 350
YDLDPISPVL DNGLFREDLP VVGGLHVSKA NEPVIEALKD SGNLVKLAKI 400
EHSYPHCWRH KTPLIFRATA QWFVSMDQAG LLPRARQEID KVQWLPEWGK 450
ARIEGMLTDR PDWCISRQRT WGVPIALFVN KETSELHPQT PALIEQVAQR 500
VEKAGVDAWF DLDPAELLGD EADQYSKVTD TLDVWFDSGV THYCVLDQRE 550
QLRAPADLYL EGSDQHRGWF QSSLLTSLAI RDAAPYSTVL THGFTVDEHG 600
RKMSKSVGNV IAPQEVWNDL GADILRLWVC ATDYRGEMSV SKDILKQMGD 650
SYRRIRNTSR FLLSNLSGFE PATDALQPEQ MLALDRYIVD RALQVQAEIQ 700
DLYDGYHFHQ VYQKLHNFCA LDLGGFYLDI IKDRQYTTQA DSVARRSCQT 750
ALYHIAQALV RWMAPVLSFT AEEIYENLPG ERLDSVFLAE WYDGLFALAD 800
NADMGRAFWD KVQDAKQAVN KAIEGARAAK LIKGSLSAEV VLFVDAEQNA 850
LLQRLGDELR FVTITSAAVL KPLAEAPAEL EDTSVAGLKV QVLASDHAKC 900
ARCWHHQPDV GSHAEHPELC GRCITNVEGD GEVRHYA 937
Length:937
Mass (Da):104,314
Last modified:September 5, 2006 - v1
Checksum:iDB713DE4653A6E4C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM286690 Genomic DNA. Translation: CAL15907.1.
RefSeqiYP_692179.1. NC_008260.1.

Genome annotation databases

EnsemblBacteriaiCAL15907; CAL15907; ABO_0459.
GeneIDi4211348.
KEGGiabo:ABO_0459.
PATRICi20838551. VBIAlcBor124741_0481.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM286690 Genomic DNA. Translation: CAL15907.1 .
RefSeqi YP_692179.1. NC_008260.1.

3D structure databases

ProteinModelPortali Q0VSE1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 393595.ABO_0459.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL15907 ; CAL15907 ; ABO_0459 .
GeneIDi 4211348.
KEGGi abo:ABO_0459.
PATRICi 20838551. VBIAlcBor124741_0481.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci ABOR393595:GHRI-471-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SK2 / ATCC 700651 / DSM 11573.

Entry informationi

Entry nameiSYI_ALCBS
AccessioniPrimary (citable) accession number: Q0VSE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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