Reviewed, UniProtKB/Swiss-Prot Q0VSB5 (ILVC_ALCBS)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	ABO_0485

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000252748
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0VSB5-1 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 3E503EF2FAFB470F	FASTA	338	36,297
	10 20 30 40 50 60 MQVYYDKDCD LSIIQGKKVA ILGYGSQGHA HACNLKDSGV DVVVGLRTGS TSIAKAEAHG 70 80 90 100 110 120 LSVTDVPSAV AAADVVMVLT PDEFQAHLYK SDIEPNLKEG ATLAFAHGFA IHYNQIVPRA 130 140 150 160 170 180 DLDVIMIAPK APGHTVRTEF TKGGGIPDLI AIFQDASGSA KELALSYACG VGGGRTGIIE 190 200 210 220 230 240 TTFKDETETD LFGEQAVLCG GAVELVKAGF ETLTEAGYAP EMAYFECLHE LKLIVDLMYE 250 260 270 280 290 300 GGIANMNYSI SNNAEYGEYV TGPEVINDES RAAMRNALKR IQSGEYAKMF IAEGAHNYPS 310 320 330 MTAARRNNAA HPIEQVGEKL RGMMPWIQAN QIVDKTKN

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL15933.1. Different initiation.
RefSeq	YP_692205.1.
3D structure databases
SMR	Q0VSB5. Positions 1-327.
ModBase	Search...
Genome annotation databases
GeneID	4211352.
GenomeReviews	Gene locus ABO_0485 in contig AM286690_GR.
KEGG	abo:ABO_0485.
NMPDR	fig|393595.12.peg.482.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VSB5.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0485-MON.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_ALCBS

Accession

Primary (citable) accession number: Q0VSB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)