Q0VSA5 (AMPA_ALCBS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	ABO_0495

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000071653
Sites
	Active site	272	1	Potential
	Active site	346	1	Potential
	Metal binding	260	1	Manganese 2 By similarity
	Metal binding	265	1	Manganese 1 By similarity
	Metal binding	265	1	Manganese 2 By similarity
	Metal binding	283	1	Manganese 2 By similarity
	Metal binding	342	1	Manganese 1 By similarity
	Metal binding	344	1	Manganese 1 By similarity
	Metal binding	344	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VSA5 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 8D157C58349417BF	FASTA	489	52,501
	10 20 30 40 50 60 MDFAVSNRPL EKSKADALVV LLGKKNDLPQ ALPSATREQI SQFTKAGDFN ASKGQLVWLH 70 80 90 100 110 120 APQDLNADRL LLVGTGDSPL SDQGWLSLVH KATKTLASSP VKQAMWLLDD THTQTLEWQV 130 140 150 160 170 180 REGSRVVEES TYRFDNYRSK PAPASNLAKL TFWHAEKDTT LTKAHKTGKA VGLGVNVARD 190 200 210 220 230 240 LGNLPPNDCY PEYLSGVARD LGKEYEKLTV KVLSQAQAEK MGMGAFHAVA KGSERQGQII 250 260 270 280 290 300 VMEYKGAKPT KQGPVALVGK GITFDTGGIS LKPGATMDEM KYDMGGAASV FGSVKTVCEL 310 320 330 340 350 360 DLPIHLVAVV AAAENMPDGR AARPGDIVKT LSGQTVEILN TDAEGRLVLC DALTYVQQKH 370 380 390 400 410 420 KPHTVIDIAT LTGACVVALG AHAQAVYSND DDLSEALLAA GKETGDRGWP MPLWDEYQGQ 430 440 450 460 470 480 LDSPFADMQN IGGPKAGSIT AACFLSRFTK EVKWAHLDIA GTAWISGGMS KGATGRPVPM LTRYLMDNA

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SK2 / ATCC 700651 / DSM 11573.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286690 Genomic DNA. Translation: CAL15943.1.
RefSeq	YP_692215.1. NC_008260.1.
3D structure databases
ProteinModelPortal	Q0VSA5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VSA5.
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4212452.
GenomeReviews	Gene locus ABO_0495 in contig AM286690_GR.
KEGG	abo:ABO_0495.
NMPDR	fig|393595.12.peg.492.
PATRIC	20838629. VBIAlcBor124741_0517.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0260.
HOGENOM	HBG742580.
OMA	MPANICT.
PhylomeDB	Q0VSA5.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0495-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ALCBS

Accession

Primary (citable) accession number: Q0VSA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program