Reviewed, UniProtKB/Swiss-Prot Q0VS07 (MURE_ALCBS)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	ABO_0593

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_1000058583
Regions
	Nucleotide binding	112 – 118	7	ATP Potential
	Region	154 – 155	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	406 – 409	4	Meso-diaminopimelate binding By similarity
	Motif	406 – 409	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	27	1	UDP-MurNAc-L-Ala-D-Glu; via carbonyl oxygen By similarity
	Binding site	29	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	181	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	187	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	189	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	382	1	Meso-diaminopimelate By similarity
	Binding site	457	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	461	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	221	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VS07-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 659B7856B35036E4	FASTA	490	51,222
	10 20 30 40 50 60 MMTLQQLLPN TTLPATAAGV VLSGLQLDSR KVMPGDAFLA VQGVASDGRQ FVSQAIAAGA 70 80 90 100 110 120 VAVLAEAETF SVQQEQAVPR VNFPGLSRSV GELAARWYGE PGSAMNVVGV TGTNGKTSIT 130 140 150 160 170 180 WFLRDALNAL GHRCALVGTL GLGLKDNEQI TGHTTPDPIT LQAGLARVLE AGADTVAMEV 190 200 210 220 230 240 SSHALDQHRL GPTPVSVAVF SNLSRDHLDY HGDMEAYLAA KSVLFTRDGV KLAVINCDDP 250 260 270 280 290 300 VAYSLVGCLA DGVRCVTFGD QTGATVRCAS VAFTAEGTNA ELNVGGESFS VTLPLFGRFN 310 320 330 340 350 360 LSNLMAVAGI LHGQGVSTDS LAGALAAITP VPGRMEPVQQ AGCPTVIVDY AHTPDGLEKA 370 380 390 400 410 420 LQACRAHFAG RLFCIIGCGG DRDTGKRPQM AAVAERGADH VVFTSDNPRS EDPQKIIDEM 430 440 450 460 470 480 CAGLTDTGSV QIIVERDRAV MDTVQAASDG DVILLAGKGH EDYQEVNGVR YPSDDRDLAH 490 KAFNARGGAQ

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL16041.1.
RefSeq	YP_692313.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4211243.
GenomeReviews	Gene locus ABO_0593 in contig AM286690_GR.
KEGG	abo:ABO_0593.
NMPDR	fig|393595.12.peg.591.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VS07.
OMA	Q0VS07. FPVIVDY.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0593-MON.
Family and domain databases
HAMAP	MF_00208. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_ALCBS

Accession

Primary (citable) accession number: Q0VS07

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)