Reviewed, UniProtKB/Swiss-Prot Q0VS05 (MRAY_ALCBS)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	ABO_0595

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000002931
Regions
	Transmembrane	27 – 47	21	Potential
	Transmembrane	73 – 93	21	Potential
	Transmembrane	97 – 117	21	Potential
	Transmembrane	132 – 152	21	Potential
	Transmembrane	164 – 184	21	Potential
	Transmembrane	199 – 219	21	Potential
	Transmembrane	236 – 256	21	Potential
	Transmembrane	263 – 283	21	Potential
	Transmembrane	288 – 308	21	Potential
	Transmembrane	337 – 357	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0VS05-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: A0F25F57BB510943	FASTA	360	39,339
	10 20 30 40 50 60 MLLWLAEYLA QYHSGFLVVQ YITLRGILSV LTALFIAFWV GPIMIRMLQE KQVGQAIRDD 70 80 90 100 110 120 GPKSHLSKAG TPTMGGALIL VAIVISTLLW GDLENRFVWI TLGVLFVFGA VGWVDDWRKV 130 140 150 160 170 180 VEKNPRGLPA RWKYLWLSVG ALGAGCALFF TAQSPVETQL IVPFFKSVAI NMGWFYIVLT 190 200 210 220 230 240 YFVINGTSNA VNLTDGLDGL AIMPTVLVGG ALGIFAYAGG HAEFATYLQI PYVAGAGELV 250 260 270 280 290 300 IISAALCGAG LGFLWFNAYP AQVFMGDVGA LSLGAVLGVM AVIVRQEIVL FIMGGVFVME 310 320 330 340 350 360 TVSVMLQVGS FKLTGRRIFR MAPIHHHFEL KGWPEPKIIV RFWIITVILV LVGLATLKIR

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL16043.1.
RefSeq	YP_692315.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VS05.
Genome annotation databases
GeneID	4211247.
GenomeReviews	Gene locus ABO_0595 in contig AM286690_GR.
KEGG	abo:ABO_0595.
NMPDR	fig|393595.12.peg.593.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VS05.
OMA	HHHFEQK.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0595-MON.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_ALCBS

Accession

Primary (citable) accession number: Q0VS05

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)