Reviewed, UniProtKB/Swiss-Prot Q0VS01 (MURC_ALCBS)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	ABO_0599

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000004306
Regions
	Nucleotide binding	123 – 129	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0VS01-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: D4EDC6BF5E857C73	FASTA	474	51,161
	10 20 30 40 50 60 MADNDIIHTV PEMRRIRGIH FVGIGGVGMC GIAEVLANQG YAISGSDIKE SPVLERLRSL 70 80 90 100 110 120 GVRVDIGHRA ENIDGADVVV TSTAVNTENL EVAAAHERRI PVVPRAQMLA ELMRFRHGIA 130 140 150 160 170 180 VAGTHGKTTT TSLTAAIMAE AGLDPTFVIG GRLNSAGTNA RLGQSRYLVA EADESDASFL 190 200 210 220 230 240 HLQPMSAIVT NVDADHMHTY GGDFAQLENT FIEFLHNLPF YGVAVMCVDD PVVRKLLPRV 250 260 270 280 290 300 NRQVIRYGFS EDADLRAENV RQDGMVTHFR VVRAEGEPLD VSLNMPGRHN VLNALAAIAV 310 320 330 340 350 360 SADEGAGDEA IIRGLNNFTG VGRRFDVQGD YHFDGGSATL VDDYGHHPRE VAATIDAIRE 370 380 390 400 410 420 GWPGRRLAML FQPHRYTRTQ DLYEDFVEVL SGVDVLLMLE VYAAGEEPIP GADARALCRS 430 440 450 460 470 LRKRGLEPVF VDDPDKLQGL LASQLQNGDL LVTQGAGNVG AIAKQLVAQG GGHG

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL16047.1.
RefSeq	YP_692319.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4211241.
GenomeReviews	Gene locus ABO_0599 in contig AM286690_GR.
KEGG	abo:ABO_0599.
NMPDR	fig|393595.12.peg.597.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VS01.
OMA	Q0VS01. EWMVVEA.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0599-MON.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ALCBS

Accession

Primary (citable) accession number: Q0VS01

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)