Q0VRN6 (GLPK_ALCBS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	ABO_0714

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Glycerol kinase HAMAP MF_00186		PRO_1000020695
Regions
	Nucleotide binding	407 – 411	5	ATP By similarity
Sites
	Binding site	11	1	Substrate By similarity
	Binding site	15	1	ATP By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	242	1	Substrate By similarity
	Binding site	264	1	ATP By similarity
	Binding site	306	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VRN6 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: B369FC409141A4DA	FASTA	497	54,056
	10 20 30 40 50 60 MPYILSIDQG TTSSRAIVFD ANGHACGQAQ KEFRQYFPED GWVEHDAMEI WNDTLAMCQQ 70 80 90 100 110 120 ALRNARVEAQ QLVAIGITNQ RETTVLWDRE TGDPLARAIV WQDRRTASTC EALRDQGHEN 130 140 150 160 170 180 QVRSKTGLLL DPYFSATKLA WLLDNVPNAR QRAEAGELAF GTIDSWLLWQ LTRGKVHATD 190 200 210 220 230 240 ATNASRTLLF NIHEQCWDEE LLTLFNVPAS VLPDVRDSAA DFGTTCPELL GAAVPVTGIA 250 260 270 280 290 300 GDQQAALVGQ ACFAPGMVKS TYGTGCFMVM NTGEAVESHN RLLTTVGYRL NGKTTYALEG 310 320 330 340 350 360 SIFVAGAAIQ WLRDGLHLIR DARETEALAR RVGSAGGVYL VPAFTGLGAP WWDPHARGAL 370 380 390 400 410 420 MGLTRDTGIA EVVTAGLEAV CYQSRDLLDA MAADCGTRPT TLRVDGGMVV NNWLSQTLSD 430 440 450 460 470 480 VLGVCVDRPV VTETTALGAA YLAGLGVGLY ASLESIAEQW RCERGFSPAL AEPERQKRYQ 490 GWRDAVARVC QTSRGAN

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SK2 / ATCC 700651 / DSM 11573.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286690 Genomic DNA. Translation: CAL16162.1.
RefSeq	YP_692434.1. NC_008260.1.
3D structure databases
ProteinModelPortal	Q0VRN6.
SMR	Q0VRN6. Positions 3-490.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VRN6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4211193.
GenomeReviews	Gene locus ABO_0714 in contig AM286690_GR.
KEGG	abo:ABO_0714.
NMPDR	fig|393595.12.peg.715.
PATRIC	20839103. VBIAlcBor124741_0751.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HBG511469.
OMA	KMAGSAQ.
PhylomeDB	Q0VRN6.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0714-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_ALCBS

Accession

Primary (citable) accession number: Q0VRN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program